Patents by Inventor Jia-Wang Wang
Jia-Wang Wang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11530424Abstract: The clustered regularly interspaced short palindromic repeat (CRISPR) gene editing technique, based on the non-homologous end-joining (NHEJ) repair pathway, can efficiently generate gene knockouts of variably sizes. More precise genome editing, either the insertion or deletion of a desired fragment, can be done by combining the homology-directed-repair (HDR) pathway with CRISPR cleavage. HDR-mediated gene knock-in experiments are inefficient, with no reports of successful gene knock-in with DNA fragments larger than 4 kb. Targeted insertion of large DNA fragments (7.4 and 5.8 kb) into the genomes of mouse embryonic stem cells and zygotes, respectively, using the CRISPR/HDR technique without NHEJ inhibitors was performed and indicate that CRISPR/HDR without NHEJ inhibitors can result in highly efficient gene knock-in, equivalent to CRISPR/HDR with NHEJ inhibitors.Type: GrantFiled: September 30, 2019Date of Patent: December 20, 2022Assignees: University of South Florida, United States Department of Veterans AffairsInventors: Jia-Wang Wang, Richard F. Lockey
-
Patent number: 10654918Abstract: Anti-cytokine therapy has revolutionized immunological disease treatment, but is not always effective and subject to treatment resistance as the cytokine cascade is highly redundant and multiple cytokines are involved in inflammation. Targeting a critical common regulator of inflammatory effectors is desirable. Lipopolysaccharide (LPS)-responsive beige-like anchor (LRBA) is a master regulator of multiple genes important for inflammation. Subcellular localization shows that LRBA translocated to the nucleus upon LPS stimulation and colocalized with multiple proteins associated with the endosome membrane system, indicating a critical role in membrane/vesicle trafficking essential for deposition, secretion and signal transduction of immune effectors.Type: GrantFiled: July 27, 2017Date of Patent: May 19, 2020Assignee: University of South FloridaInventors: Jia-Wang Wang, Richard F. Lockey
-
Publication number: 20180362989Abstract: Provided herein are methods of cloning into vectors.Type: ApplicationFiled: June 11, 2018Publication date: December 20, 2018Applicant: University of South FloridaInventors: JIA-WANG WANG, RICHARD F. LOCKEY, KUNYU LI
-
Patent number: 10030263Abstract: Described herein are multiplexed RNA, including miRNA, PCR-based assays.Type: GrantFiled: August 24, 2015Date of Patent: July 24, 2018Assignee: University of South FloridaInventors: Jia-Wang Wang, Richard F. Lockey
-
Patent number: 9974290Abstract: The invention concerns a non-human animal model useful for sensitively studying gene-gene interactions over a wide genetic background; methods for producing the animal model; and methods for studying gene-gene interactions using an animal model of the invention.Type: GrantFiled: April 18, 2016Date of Patent: May 22, 2018Assignee: UNIVERSITY OF SOUTH FLORIDAInventors: Jia-Wang Wang, Richard F. Lockey
-
Publication number: 20180002413Abstract: Anti-cytokine therapy has revolutionized immunological disease treatment, but is not always effective and subject to treatment resistance as the cytokine cascade is highly redundant and multiple cytokines are involved in inflammation. Targeting a critical common regulator of inflammatory effectors is desirable. Lipopolysaccharide (LPS)-responsive beige-like anchor (LRBA) is a master regulator of multiple genes important for inflammation. Subcellular localization shows that LRBA translocated to the nucleus upon LPS stimulation and colocalized with multiple proteins associated with the endosome membrane system, indicating a critical role in membrane/vesicle trafficking essential for deposition, secretion and signal transduction of immune effectors.Type: ApplicationFiled: July 27, 2017Publication date: January 4, 2018Applicant: University of South FloridaInventors: Jia-Wang Wang, Richard F. Lockey
-
Publication number: 20180002706Abstract: Provided herein are methods of cloning into vectors.Type: ApplicationFiled: December 18, 2015Publication date: January 4, 2018Applicant: University of South FloridaInventors: JIA-WANG WANG, RICHARD F. LOCKEY, KUNYU LI
-
Patent number: 9738706Abstract: Anti-cytokine therapy has revolutionized immunological disease treatment, but is not always effective and subject to treatment resistance as the cytokine cascade is highly redundant and multiple cytokines are involved in inflammation. Targeting a critical common regulator of inflammatory effectors is desirable. Lipopolysaccharide (LPS)-responsive beige-like anchor (LRBA) is a master regulator of multiple genes important for inflammation. Subcellular localization shows that LRBA translocated to the nucleus upon LPS stimulation and colocalized with multiple proteins associated with the endosome membrane system, indicating a critical role in membrane/vesicle trafficking essential for deposition, secretion and signal transduction of immune effectors.Type: GrantFiled: March 24, 2014Date of Patent: August 22, 2017Assignee: University of South FloridaInventors: Jia-Wang Wang, Richard F. Lockey
-
Publication number: 20170188554Abstract: The invention concerns a non-human animal model useful for modeling complex human diseases; compositions comprising cell populations from the animal model having different genotypes for the same gene; methods for producing the animal model; and methods for studying a phenotype using an animal model or compositions of the invention.Type: ApplicationFiled: April 18, 2016Publication date: July 6, 2017Applicant: UNIVERSITY OF SOUTH FLORIDAInventors: JIA-WANG WANG, RICHARD F. LOCKEY
-
Patent number: 9493825Abstract: The present invention provides materials and methods for detecting, quantifying, and/or high-throughput-profiling microRNAs. Advantageously, the present invention is more sensitive and specific than other currently-available miRNA qPCR assays. In addition, the present invention is convenient, easy-to-perform, and cost-effective. In one embodiment, the present invention provides a universal primer for reverse transcription of all miRNAs, a universal reverse primer for PCR amplification reaction, and universal probes. In another embodiment, the present invention provides assays that allow simultaneous detection and/or quantification of a plurality of target miRNAs using a single reverse transcription reaction.Type: GrantFiled: March 16, 2012Date of Patent: November 15, 2016Assignee: UNIVERSITY OF SOUTH FLORIDAInventors: Shyam S. Mohapatra, Jia-Wang Wang
-
Publication number: 20160302398Abstract: The invention concerns a non-human animal model useful for sensitively studying gene-gene interactions over a wide genetic background; methods for producing the animal model; and methods for studying gene-gene interactions using an animal model of the invention.Type: ApplicationFiled: April 18, 2016Publication date: October 20, 2016Applicant: UNIVERSITY OF SOUTH FLORIDAInventors: JIA-WANG WANG, RICHARD F. LOCKEY
-
Publication number: 20160160274Abstract: The present invention provides materials and methods for detecting, quantifying, and/or high-throughput-profiling microRNAs. Advantageously, the present invention is more sensitive and specific than other currently-available miRNA qPCR assays. In addition, the present invention is convenient, easy-to-perform, and cost-effective. In one embodiment, the present invention provides a universal primer for reverse transcription of all miRNAs, a universal reverse primer for PCR amplification reaction, and universal probes. In another embodiment, the present invention provides assays that allow simultaneous detection and/or quantification of a plurality of target miRNAs using a single reverse transcription reaction.Type: ApplicationFiled: December 8, 2015Publication date: June 9, 2016Applicant: UNIVERSITY OF SOUTH FLORIDAInventors: SHYAM S. MOHAPATRA, JIA-WANG WANG
-
Publication number: 20160108108Abstract: Anti-cytokine therapy has revolutionized immunological disease treatment, but is not always effective and subject to treatment resistance as the cytokine cascade is highly redundant and multiple cytokines are involved in inflammation. Targeting a critical common regulator of inflammatory effectors is desirable. Lipopolysaccharide (LPS)-responsive beige-like anchor (LRBA) is a master regulator of multiple genes important for inflammation. Subcellular localization shows that LRBA translocated to the nucleus upon LPS stimulation and colocalized with multiple proteins associated with the endosome membrane system, indicating a critical role in membrane/vesicle trafficking essential for deposition, secretion and signal transduction of immune effectors.Type: ApplicationFiled: March 24, 2014Publication date: April 21, 2016Applicant: UNIVERSITY OF SOUTH FLORIDAInventors: Jia-Wang Wang, Richard F. Lockey
-
Publication number: 20160053319Abstract: MicroRNAs are shown to be up- and/or down-regulated in inflammation and immune cells using a mouse model of asthma and regulatory T cells as source of RNA, respectively. Modulating the expression of these microRNAs can be effective in redirecting inflammation and immunity and hence, can be beneficial as biomarkers or as therapeutic agents against diverse human immunologic and inflammatory diseases.Type: ApplicationFiled: June 23, 2015Publication date: February 25, 2016Applicant: UNIVERSITY OF SOUTH FLORIDAInventors: SHYAM S. MOHAPATRA, JIA-WANG WANG
-
Patent number: 9089589Abstract: MicroRNAs are shown to be up- and/or down-regulated in inflammation and immune cells using a mouse model of asthma and regulatory T cells as source of RNA, respectively. Modulating the expression of these microRNAs can be effective in redirecting inflammation and immunity and hence, can be beneficial as biomarkers or as therapeutic agents against diverse human immunologic and inflammatory diseases.Type: GrantFiled: February 20, 2013Date of Patent: July 28, 2015Assignee: UNIVERSITY OF SOUTH FLORIDAInventors: Shyam S. Mohapatra, Jia-Wang Wang
-
Patent number: 8821907Abstract: N-halaminehydantoinyl epoxide compounds which can be used for the construction of coatings and materials which can be rendered biocidal by exposure to halogen solutions either before or after curing the coating or material are disclosed. The biocidal coatings and materials can then be used to inactivate pathogenic microorganisms such as bacteria, fungi, and yeasts, as well as virus particles, which can cause infectious diseases, and those microorganisms which cause noxious odors and unpleasant coloring such as mildew. The coatings are compatible with a variety of substrates including, but not limited to, cellulose, chitin, chitosan, synthetic fibers, cement grout, latex caulk, acrylic films, polyurethanes, plastics and paints.Type: GrantFiled: March 10, 2006Date of Patent: September 2, 2014Assignee: Auburn University Office of Technology TransferInventors: Shelby D. Worley, Jie Liang, Yongjun Chen, Royall M. Broughton, Jia-Wang Wang, Rong Wu, Unchin Cho, Jaewoong Lee, Kevin Barnes
-
Publication number: 20140004520Abstract: The present invention provides materials and methods for detecting, quantifying, and/or high-throughput-profiling microRNAs. Advantageously, the present invention is more sensitive and specific than other currently-available miRNA qPCR assays. In addition, the present invention is convenient, easy-to-perform, and cost-effective. In one embodiment, the present invention provides a universal primer for reverse transcription of all miRNAs, a universal reverse primer for PCR amplification reaction, and universal probes. In another embodiment, the present invention provides assays that allow simultaneous detection and/or quantification of a plurality of target miRNAs using a single reverse transcription reaction.Type: ApplicationFiled: March 16, 2012Publication date: January 2, 2014Inventors: Shyam S. Mohapatra, Jia-Wang Wang
-
Patent number: 8415096Abstract: MicroRNAs are shown to be up- and/or down-regulated in inflammation and immune cells using a mouse model of asthma and regulatory T cells as source of RNA, respectively. Modulating the expression of these microRNAs can be effective in redirecting inflammation and immunity and hence, can be beneficial as biomarkers or as therapeutic agents against diverse human immunologic and inflammatory diseases.Type: GrantFiled: May 23, 2008Date of Patent: April 9, 2013Assignee: University of South FloridaInventors: Shyam S. Mohapatra, Jia-Wang Wang
-
Publication number: 20130045885Abstract: The present invention provides materials and methods for detecting, quantifying, and/or profiling microRNAs. Advantageously, the present invention is sensitive, specific, convenient, and cost-effective. In one embodiment, the present invention provides a universal primer for reverse transcription of miRNAs, a universal reverse primer for PCR amplification reaction, and a universal probe. In another embodiment, the present invention provides assays that allow the detection and/or quantification of a plurality of target miRNAs using a single reverse transcription reaction and a single qPCR reaction.Type: ApplicationFiled: February 18, 2011Publication date: February 21, 2013Applicant: Univeristy of South FloridaInventors: Shyam S. Mohapatra, Jia-Wang Wang
-
Publication number: 20120289420Abstract: The present invention provides miRNA biomarkers useful for diagnosis, prognosis, and/or treatment of airway diseases such as asthma, asthma exacerbation and nasal polyps.Type: ApplicationFiled: March 19, 2012Publication date: November 15, 2012Applicant: UNIVERSITY OF SOUTH FLORIDAInventors: SHYAM S. MOHAPATRA, JIA-WANG WANG