Abstract: An extracellular matrix-modified decellularized nerve scaffold and use thereof are provided. The extracellular matrix-modified decellularized nerve scaffold is prepared from a natural porcine optic nerve. The scaffold has a plurality of longitudinal channels and a plurality of transversal foramina intercommunicated with the longitudinal channels, which have relatively uniform diameters and relatively even distributions in the scaffold. The extracellular matrix-modified decellularized optic nerve scaffold of the present invention changes the poor microenvironment of existing decellularized material that lacks cell growth factors and nutrients, supports seeded cells to form neural networks in vitro or in vivo, and enables the connection of ascending nerve fibers or descending nerve fibers of the injured spinal cord to their target cells after transplantation.