Abstract: A recombinant collagen and a preparation method and use thereof, and in particular, a full-length collagen ?1 chain produced through recombinant expression, and a preparation method and use thereof are provided. When a variant (which is denoted as ?1 (I) M1) of a human type I collagen ?1 chain (?1 (I) chain) and a variant (which is denoted as ?1 (II) M6) of a human type II collagen ?1 chain (?1 (II) chain) constructed in the present disclosure are produced through recombinant expression in Pichia pastoris, a main degradation product (a main degradation band) in substantially the same proportion as a target product (a target band) of a full-length ?1 chain occurring during the recombinant expression of native full-length ?1 (I) chain and ?1 (II) chain is eliminated, and a yield of the target product is improved.

Abstract: Embodiments of the present disclosure provide an image processing method, an apparatus, an electronic device, and a storage medium. The method comprises: determining retrieval information corresponding to at least one image to be used; obtaining a target parameter to be sent to a first serving end by processing text descriptive information of the image to be used and the retrieval information, to cause the first serving end to perform image processing based on the target parameter.

Abstract: The present invention relates to the technical field of microorganisms, and specifically to engineered microorganisms expressing acetoacetyl-CoA reductase variants and methods for increasing the proportion of 3-hydroxyhexanoic acid in PHA. The acetoacetyl-CoA reductase variants and their coding genes provided by the present invention can significantly increase the proportion of 3-hydroxyhexanoic acid in PHA produced by strains; the proportion of 3-hydroxyhexanoic acid in PHA produced by the engineered Ralstonia eutropha constructed utilizing the acetoacetyl-CoA reductase variants and their coding genes provided by the present invention is significantly increased, which provides new genes and strain resources for strains producing poly(3-hydroxybutyrate-co-3-10 the development of engineered hydroxyhexanoate) with high proportion of 3-hydroxyhexanoic acid.

Abstract: Implementations of the present specification disclose a fare payment method, apparatus, and device. In one aspect, the method includes: receiving, at a terminal device supporting host-based card emulation (HCE), an HCE-based routing instruction from a fare collection device of a public transportation system; establishing, by the terminal device, a near field communication (NFC) connection with the fare collection device in response to the routing instruction; and transmitting, by the terminal device, payment-related information of a user of the terminal device to the fare collection device through the NFC connection, wherein the payment-related information is to be verified by the fare collection device, and wherein the fare collection device is configured to: verify the received payment-related information of the user; and in response to a successful verification, deduct a fare from an account of the user based on the payment-related information.

Abstract: A recombinant collagen and a preparation method and use thereof, and in particular, a full-length collagen a1 chain produced through recombinant expression, and a preparation method and use thereof are provided. When a variant (which is denoted as a1 (I) M1) of a human type I collagen a1 chain (a1 (I) chain) and a variant (which is denoted as a1 (II) M6) of a human type II collagen a1 chain (a1 (II) chain) constructed in the present disclosure are produced through recombinant expression in Pichia pastoris, a main degradation product (a main degradation band) in substantially the same proportion as a target product (a target band) of a full-length a1 chain occurring during the recombinant expression of native full-length a1 (I) chain and a1 (II) chain is eliminated, and a yield of the target product is improved.

Abstract: A data caching and reading method, and a data access system are provided. A client sends a first request to a data storage system so as to obtain metadata of target data. The client sends a second request to a data caching system, the second request comprising the metadata. The data caching system determines, on the basis of the metadata, whether cache target data consistent with the target data stored in the data storage system is cached. When it is determined cache target data is cached, the client obtains the target data from the data caching system. When no consistent cache target data is cached, the client obtains the target data from the data storage system, and sends the obtained target data to the data caching system, so as to cache the target data.

Abstract: The present disclosure provides a multimedia data processing method, apparatus, and electronic device, the method is performed by a terminal device, in operation, whether or not a shield instruction for playing multimedia data is received is detected, and the shield instruction indicates that the target parameters meet the preset conditions. Multimedia data is shielded. If the shield instruction is received, the target parameters in the multimedia data to be played are detected. If the target parameters in the multimedia data to be played meet the preset conditions, the multimedia data to be played is shielded.

Abstract: The invention discloses a method for purifying a retrovirus (retroviral vector), comprising the following steps: A), microfiltrating a cell culture supernatant containing a retrovirus to remove cell debris from the cell culture supernatant and obtain a micro-filtered virus solution; B), treating the micro-filtered virus solution with a nuclease to degrade host DNA residues into small DNA fragments, and obtain an enzyme-digested virus solution; C), ultrafiltering the enzyme-digested virus solution such that the retrovirus is retained in a retentate, collecting the retentate, and obtaining an ultrafiltrated virus solution; and D), performing low speed centrifugation on the ultrafiltered virus solution to precipitate the retrovirus, collecting the precipitate to obtain unsterilized virus.

Abstract: The present invention relates to the technical field of microorganisms, and specifically to engineered microorganisms expressing acetoacetyl-CoA reductase variants and methods for increasing the proportion of 3-hydroxyhexanoic acid in PHA. The acetoacetyl-CoA reductase variants and their coding genes provided by the present invention can significantly increase the proportion of 3-hydroxyhexanoic acid in PHA produced by strains; the proportion of 3-hydroxyhexanoic acid in PHA produced by the engineered Ralstonia eutropha constructed utilizing the acetoacetyl-CoA reductase variants and their coding genes provided by the present invention is significantly increased, which provides new genes and strain resources for strains producing poly(3-hydroxybutyrate-co-3-10 the development of engineered hydroxyhexanoate) with high proportion of 3-hydroxyhexanoic acid.

Abstract: The present disclosure relates to a push method, push apparatus of video and storage medium, and relates to the technical field of multimedia technologies. The push method of video includes: acquiring videos comprising video(s) with a photosensitive tag and video(s) without a photosensitive tag, wherein each video has a photosensitive tag if it is determined as a photosensitive video by a photosensitive video recognition model; and pushing video(s) that do not have a photosensitive tag among the videos to a browsing user in case that the browsing user is in a state of photosensitive video filtering.

Abstract: A recombinant human type XVII collagen consists of an amino acid sequence shown in (A)n or includes the amino acid sequence shown in (A)n, where A is a sequence set forth in SEQ ID NO: 2, a sequence undergoing an amino acid modification to a predetermined extent based on SEQ ID NO: 2, or a sequence that has more than 80% homology with SEQ ID NO: 2; n is an integer greater than or equal to 1; and A represents a basic unit, and when there are two or more basic units, the two or more basic units are identical or different and are directly connected in tandem through a peptide bond. In the present disclosure, it is confirmed that the recombinant human type XVII collagen can undergo efficient secretory and soluble expression in eukaryotic host cells such as Pichia pastoris (P. pastoris).

Abstract: The disclosure relates to the technical field of soybean breeding, in particular to a method for improving soybean hybrid yield. In the method, fertile soybean is taken as a male parent, and pollen from the male parent is prepared into a pollen compound solution, which is carried with a spraying device to be directionally spayed to a female parent for hybridization, where the female parent is a genic male sterile soybean plant. By preparation of an appropriate proportion of pollen compound solution and pollination assisted by artificial spraying, the disclosure may not only enhance the pollen activity, but also improve the pollen utilization rate and greatly improve the efficiency of soybean hybrid production, thereby improving the soybean yield. It effectively saves human resources, improves the efficiency of soybean hybrid production, and meets the needs of soybean industry development.

Abstract: A recombinant human type XVII collagen consists of an amino acid sequence shown in (A)n or includes the amino acid sequence shown in (A)n, where A is a sequence set forth in SEQ ID NO: 2, a sequence undergoing an amino acid modification to a predetermined extent based on SEQ ID NO: 2, or a sequence that has more than 80% homology with SEQ ID NO: 2; n is an integer greater than or equal to 1; and A represents a basic unit, and when there are two or more basic units, the two or more basic units are identical or different and are directly connected in tandem through a peptide bond. In the present disclosure, it is confirmed that the recombinant human type XVII collagen can undergo efficient secretory and soluble expression in eukaryotic host cells such as Pichia pastoris (P. pastoris).

Abstract: Generating verification codes includes selecting at least two verification code generators from a verification code generator set comprising a plurality of verification code generators to compose a current use set, executing each verification code generator in the current use set to obtain corresponding partial verification codes, composing a current verification code from the partial verification codes, outputting the current verification code to a user, receiving a user response that is made in response to the current verification code, and comparing the current verification code and the user response to determine whether the user is verified.

Abstract: A method for providing graphic code information is implementable by a device comprising a first module, a second module, and an interface module. The method comprises: the second module verifying through the interface module whether the first module is a trusted module; the second module receiving target user information through the interface module and verifying whether the target user information is in a logged-in state on the second module, wherein the target user information is pre-associated with the interface module; and after verifying that the first module is a trusted module and the target user information is in the logged-in state on the second module, the second module generating graphic code information and transmitting the graphic code information to the first module through the interface module.

Abstract: The invention relates to a method for preparing a vinyl ether polymer by photo-initiated polymerization, which comprises the step of: under a protective atmosphere, performing photo-initiated polymerization on a vinyl ether monomer in the presence of an organic halogenated hydrocarbon and manganese carbonyl under irradiation of light having a wavelength of 365-550 nm at ?25° C. to 25° C., to obtain a vinyl ether polymer after the reaction is completed. In the method, a vinyl ether monomer is subjected to cationic polymerization in the presence of manganese carbonyl and an organic halogenated hydrocarbon under visible light, to prepare a vinyl ether polymer with controlled molecular weight and narrow molecular weight distribution.

Abstract: The present invention discloses a new method for synthesizing a high-quality inorganic film by microwave heating, which relates to the field of preparation of inorganic materials. The method for synthesizing a high-quality inorganic film by microwave heating in the present invention allows a fine design and control of the temperature increase process during microwave heating, wherein the matrix and the synthesis solution is put into the microwave reactor at first, the temperature interval between the initial temperature and the target temperature of the synthesis solution is then divided into multiple sections, each of which sets a temperature increase rate, and then when the temperature reaches the target temperature after the designed temperature increase process, the synthesis solution reacts for a period of time at the target temperature, finally the high-quality inorganic film can be obtained after the treatment of washing and drying.

Abstract: The present disclosure provides a multimedia data processing method, apparatus, and electronic device, the method is performed by a terminal device, in peration, whether or not a shield instruction for playing multimedia data is received is detected, and the shield instruction indicates that the target parameters meet the preset conditions. Multimedia data is shielded. If the shield instruction is received, the target parameters in the multimedia data to be played are detected. If the target parameters in the multimedia data to be played meet the preset conditions, the multimedia data to be played is shielded.

Abstract: The present disclosure relates to a string matching method, a string matching apparatus, a storage medium, and an electronic device. The method can include loading a first string and obtaining position information of a node element of an AC automaton in the first string and a node position relation of the node element on the AC automaton. The method can further include creating a skip list based on the position information and the node position relation, performing a depth-first traversal on the AC automaton, and obtaining a first matching result of a path between each target node and a parent node of the target node and the first string based on the skip list. Further, the method can include outputting a matching result of the first string and the preset matching rule based on the first matching result of each path included in the AC automaton and the first string.

Abstract: A display panel being bendable around a bending axis to form a bendable portion, includes a support film, a display module disposed on a side of the support film. An outer edge of the display module is distant from an outer edge along the first direction of the support film, to form a step portion between the outer edge of the display module and the outer edge of the support film. A polarizing layer and a touch layer are disposed on the other side, facing away the support film, of the display module. An outer edge of the polarizing layer and an outer edge of the touch layer are distant from the outer edge of the display module to form a step portion between the outer edge of the polarizing layer, the outer edge of the touch layer and the outer edge of the display module.