Abstract: Disclosed herein are a Cas12i polypeptide and use thereof in a CRISPR-Cas system. Specifically disclosed herein are a Cas12i polypeptide, a Cas12i fusion polypeptide, a guide RNA, a complex formed by the Cas12i polypeptide or fusion polypeptide with the guide RNA, a nucleic acid, a vector, a vector system, a delivery system, a kit, a composition, and a method for modifying a nucleic acid using the components described above.

Abstract: The provided is a novel heat-induction decoking device and method tailored for the thermal conversion of solid fuels. The efficient heat-induction decoking device for the thermal conversion of the solid fuels includes: a decoking tank body, which is configured to condense a macromolecular tar in a pyrolysis gas and discharge the condensed tar from a bottom to a tar tank for storage; an electromagnetic induction heating system, which is disposed on an outer wall surface of the decoking tank body to heat the outer wall surface of the decoking tank body; an ultrasonic vibration decoking system, which is disposed on the decoking tank body to enable the decoking tank body to generate vibration by ultrasonic wave so as to enhance tar fluidity. This innovative design not only reduces energy consumption and equipment wear but also extends system operational lifespan, thereby offering substantial improvements in economic and environmental sustainability.