Abstract: Disclosed herein are panels related to the diagnosis of diseased tissue in a subject. The disclosed panels and related methods are used to predict or assess whether a subject has a neurodegenerative disorder taking into account the age of the subject. Some embodiments of the methods include applying a gene filter based on the age of the subject and generating an output of gene expression data which takes into account differences in gene profiles seen in tissues as they age.

Abstract: Diverse cDNA libraries derived from cell-free mRNA and methods of preparing the same are provided. The library may be prepared by extracting RNA from a bodily fluid such as serum or plasma, separating the RNA from contaminants, synthesizing cDNA with a reverse transcriptase enzyme, and enriching protein-coding nucleotide sequences. The library may include a multiplicity of transcripts from solid tissues. Cf-RNA can be measured by qPCR, sequencing, or other suitable methods.

Abstract: Described herein are methods and systems for monitoring a disease state of a subject's bone marrow. Further, disclosed herein are methods and systems for monitoring a treatment state of a subject's organ. Moreover, disclosed herein are methods and systems for monitoring a healthy state of a subject's bone marrow and assaying an active agent.

Abstract: Methods of assessing or determining a disease stage of a liver are provided. The methods can include obtaining a sample from a subject. The methods can also include measuring gene expression products in the sample from the subject to determine the disease stage of the liver.