Abstract: The present invention discloses a method of detecting the presence of mutated genes, mRNAs or microRNAs in a subject. The method comprises the following steps: (1) Provide a body fluid sample containing cells, circulating tumor cells (CTCs), and/or extracellular vesicles (EVs); and use an analyzer having overhang molecular beacons to measure fluorescence signals generated by interactions between the body fluid sample and the overhang molecular beacons, so as to detect the presence of the mutated genes, mRNAs or microRNA. Furthermore, a biochip comprising a gold coating substrate and tethered lipoplex nanoparticles encapsulating the overhang molecular beacons is also provided in the invention.

Abstract: The present invention discloses a method of detecting the presence of mutated genes, mRNAs or microRNAs in a subject. The method comprises the following steps: (1) Provide a body fluid sample containing cells, circulating tumor cells (CTCs), and/or extracellular vesicles (EVs); and use an analyzer having overhang molecular beacons to measure fluorescence signals generated by interactions between the body fluid sample and the overhang molecular beacons, so as to detect the presence of the mutated genes, mRNAs or microRNA. Furthermore, a biochip comprising a gold coating substrate and tethered lipoplex nanoparticles encapsulating the overhang molecular beacons is also provided in the invention.

Abstract: The present invention discloses a method of detecting the presence of mutated genes, mRNAs or microRNAs in a subject. The method comprises the following steps: (1) Provide a body fluid sample containing cells, circulating tumor cells (CTCs), and/or extracellular vesicles (EVs); and use an analyzer having overhang molecular beacons to measure fluorescence signals generated by interactions between the body fluid sample and the overhang molecular beacons, so as to detect the presence of the mutated genes, mRNAs or microRNA. Furthermore, a biochip comprising a gold coating substrate and tethered lipoplex nanoparticles encapsulating the overhang molecular beacons is also provided in the invention.

Abstract: The present invention discloses a method of detecting the presence of mutated genes, mRNAs or microRNAs in a subject. The method comprises the following steps: (1) Provide a body fluid sample containing cells, circulating tumor cells (CTCs), and/or extracellular vesicles (EVs); and use an analyzer having overhang molecular beacons to measure fluorescence signals generated by interactions between the body fluid sample and the overhang molecular beacons, so as to detect the presence of the mutated genes, mRNAs or microRNA. Furthermore, a biochip comprising a gold coating substrate and tethered lipoplex nanoparticles encapsulating the overhang molecular beacons is also provided in the invention.

Abstract: The present invention discloses a method of detecting the presence of mutated genes, mRNAs or microRNAs in a subject. The method comprises the following steps: (1) Provide a body fluid sample containing cells, circulating tumor cells (CTCs), and/or extracellular vesicles (EVs); and use an analyzer having overhang molecular beacons to measure fluorescence signals generated by interactions between the body fluid sample and the overhang molecular beacons, so as to detect the presence of the mutated genes, mRNAs or microRNA. Furthermore, a biochip comprising a gold coating substrate and tethered lipoplex nanoparticles encapsulating the overhang molecular beacons is also provided in the invention.

Abstract: The present invention provides a novel lipid-polymer hybrid nanoparticle (LPHN) biochip. The LPHN biochip comprises a gold coating substrate with a surface layer on the gold coating and a nanoparticle, wherein the nanoparticle anchors on the surface layer and encapsulates labeling moieties which comprise molecular beacons (MB), Toehold-initiated molecular beacons (Ti-MB), biomolecules or components for performing catalyzed hairpin DNA circuit (CHDC), and quantum dots. A method of detecting the presence of a disease or condition in a subject by the lipid-polymer hybrid nanoparticle biochip is also disclosed in the specification.

Abstract: The present disclosure includes an electrochemical proximity assay (ECPA) which leverages two aptamer or antibody-oligonucleotide probes and proximity-dependent DNA hybridization to move a redox active molecule near an electrically conductive base. The ECPA of the present disclosure produces rapid, quantitative results, enabling point-of-care use in the detection of biomarkers of disease.

Abstract: The present disclosure includes an electrochemical proximity assay (ECPA) which leverages two aptamer or antibody-oligonucleotide probes and proximity-dependent DNA hybridization to move a redox active molecule near an electrically conductive base. The ECPA of the present disclosure produces rapid, quantitative results, enabling point-of-care use in the detection of biomarkers of disease.

Abstract: The present disclosure includes an electrochemical proximity assay (ECPA) which leverages two aptamer or antibody-oligonucleotide probes and proximity-dependent DNA hybridization to move a redox active molecule near an electrically conductive base. The ECPA of the present disclosure produces rapid, quantitative results, enabling point-of-care use in the detection of biomarkers of disease.

Abstract: A method for implementing p2p video communication on webpage includes the following steps: a user accessing a webpage, loading a flash, and a webpage script calling the flash interface to connect to a socket server; the flash connecting to a stratus server to acquire a key and monitoring received messages; the flash sending the user name and the key to the socket server to store; the flash of the source user and the flash of the target user establishing a direct connection by using the key; the source user and the target user acquiring a video source of a local camera respectively, sending the video source to the counterpart through the established connection, and meanwhile, acquiring and displaying the video stream from the counterpart. The not only largely saves the bandwidth cost of the operator, but also greatly improves the smoothness of the video communication between users.

Abstract: The present invention relates to the field of network technology, specifically to a method for achieving P2P communication on webpage. The method includes the steps of: 1) accessing a webpage by a user, calling an interface of a flash to connect with a socket server, connecting with a stratus server to acquire a key and monitoring received messages by the flash; 2) sending a username and the key of a source user to the socket server by the flash and storing the username and the key of the source user by the socket server; 3) acquiring the key of the target user from the socket server and sending a message out via the netconnection and the key of the target user by the flash on the webpage of the source user; 4) displaying the message on the webpage of the target user after the flash of the webpage of the target user receives the message.