Abstract: An apparatus suitable for single molecule sequencing. The apparatus includes at least one nanowell, a plurality of nucleic acid immobilization moieties, and a plurality of types of nucleic acid fragments. The nanowell has an observation zone. The nucleic acid immobilization moieties are disposed in or proximate to the observation zone. The nucleic acid fragments are immobilized to the nucleic acid immobilization moieties, respectively. At least one polymerase is disposed in the observation zone. A method of sequencing nucleic acid molecules using the above-mentioned apparatus is provided.

Abstract: An apparatus suitable for single molecule sequencing. The apparatus includes at least one nanowell, a plurality of nucleic acid immobilization moieties, and a plurality of types of nucleic acid fragments. The nanowell has an observation zone. The nucleic acid immobilization moieties are disposed in or proximate to the observation zone. The nucleic acid fragments are immobilized to the nucleic acid immobilization moieties, respectively. At least one polymerase is disposed in the observation zone. A method of sequencing nucleic acid molecules using the above-mentioned apparatus is provided.

Abstract: A method for producing monosaccharides from algae includes (a) treating algae-containing water using an electrocoagulation device having an iron-based anode to induce flocculation of algae so as to obtain flocculated algae which have a trace amount of iron derived from the iron-based anode, (b) collecting the flocculated algae from the algae-containing water, and (c) subjecting the flocculated algae to an acid hydrolysis reaction in an acid solution to obtain monosaccharides. A liquid-to-solid ratio of a volume of the acid solution to a solid content of the flocculated algae is not less than 12 ml/g. The acid solution has an acid concentration not less than 3% by volume.