Abstract: A trituration device for tissue disaggregation includes a device housing having a hollow cylinder defining an opening extending from a first end of the device housing to a second end of the device housing, a rotor block positioned in the opening of the device housing, and a trituration grater positioned within the opening of the device housing to contact the rotor block. The rotor block includes a solid cylinder having a central channel and at least one surface that defines a rotor blade, and the trituration grater includes multiple angled cutting holes. The device also includes a collection vessel positioned at the second end of the device housing to collect disaggregated tissue of a tissue specimen, and an actuating stem extending through the central channel of the rotor block to rotate the rotor block in response to rotation of the actuating stem.