Abstract: The present invention generally relates to a method of producing a therapeutic protein of interest from host cells through a new re-cloning strategy.

Abstract: A method for preparing a carbon source for wastewater treatment by using kitchen waste, comprises: adding a complex microbial inoculant into the kitchen waste; and phase-change degrading the kitchen waste into a phase-change liquid under aerobic fermentation of the complex microbial inoculant; wherein the phase-change liquid is the carbon source for wastewater treatment; the complex microbial inoculant comprises: a microbial agent comprising aerobic bacteria; and an excipient comprising an Fe-based MOF (metal-organic framework) nanocomposite. According to the method, the kitchen waste is treated under aerobic fermentation to prepare the carbon source, a carbon-nitrogen ratio is greater than 40, and the carbon source is used to supplement a carbon source in a sewage treatment process of a sewage plant, which significantly improves nitrogen and phosphorus removal effects of sewage treatment in the sewage plant, and provides theoretical foundation and basis for resource utilization of the kitchen waste.

Abstract: The present invention generally relates to a method of producing a therapeutic protein of interest secreted from host cells cultured in a bioreactor through adding a carboxypeptidase to the cell culture.

Abstract: The present invention relates to new methods and processes for culturing mammalian cells with the addition of phenolic antioxidants. Performance of the cell culturing methods and processes in their various aspects result in a higher viable cell density and higher protein titer.

Abstract: In certain embodiments, this disclosure provides a method of increasing production of a recombinant polypeptide of interest, comprising: a) seeding mammalian cells in a fed-batch production bioreactor at a viable cell density of at least 5106 viable cells/ml; and b) culturing the cells under optimized culture conditions to produce the recombinant polypeptide of interest at high titer.

Abstract: In certain embodiments, this disclosure provides novel methods of increasing the viable cell density of an N-1 large-scale bioreactor cell culture, comprising culturing a host cell expressing a recombinant polypeptide of interest in a non-perfusion-based culture system, and wherein the viable cell density is increased to at least 5×106 cells/mL. In certain embodiments, the disclosure provides novel methods for large-scale production of a recombinant polypeptide of interest, comprising: (1) culturing a host cell expressing a recombinant polypeptide of interest in an N-1 stage in a non-perfusion-based culture system, wherein the viable cell density is increased to at least 5×106 cells/mL; and (2) culturing N fed-batch production cells in an enriched media with high-seed density at least 1.5×106 cells/mL, wherein the N fed-batch production cells are inoculated from the N-1 stage in a non-perfusion-based culture system.

Abstract: The present invention relates to new methods and processes for culturing mammalian cells with the addition of phenolic antioxidants. Performance of the cell culturing methods and processes in their various aspects result in a higher viable cell density and higher protein titer.

Abstract: The invention relates to a method and apparatus for controlling one or more controllable devices (5, 6, 7, 38, 39, 40) in a system. In order to provide the apparatus with a more user friendly interface to control the one or more controllable device in the system, the method comprises capturing an image (50), comprising the one or more controllable devices, displaying the captured image (50), associating local areas (105,106,107,108,109,110) in the captured image (50) to the one or more respective controllable devices (5,6,7,38,39,40) on basis of information associated with the controllable devices in the captured image, receiving a user input indicating a selected one of the local areas in the displayed image, determining a command for the one or more controllable devices from the user input associated with the selected local area; and communicating the command to the one or more controllable devices.

Abstract: The present invention relates to new methods and processes for culturing mammalian cells with the addition of phenolic antioxidants. Performance of the cell culturing methods and processes in their various aspects result in a higher viable cell density and higher protein titer.

Abstract: The present invention relates to new methods and processes for culturing mammalian cells with the addition of phenolic antioxidants. Performance of the cell culturing methods and processes in their various aspects result in a higher viable cell density and higher protein titer.

Abstract: The present disclosure provides a dimming device and a driving and dimming device, where the dimming device includes a communication unit, a microcontrol unit, a power supply unit and a connector, and the power supply unit is input with a mains voltage and outputs a voltage required by the communication unit, the communication unit receives a control signal obtained by wired or wireless transmission and outputs the control signal to the microcontrol unit, the microcontrol unit outputs a dimming signal for dimming, and the connector is detachably connected with a connector of another device.

Abstract: The present disclosure provides a dimming device and a driving and dimming device, where the dimming device includes a communication unit, a microcontrol unit, a power supply unit and a connector, and the power supply unit is input with a mains voltage and outputs a voltage required by the communication unit, the communication unit receives a control signal obtained by wired or wireless transmission and outputs the control signal to the microcontrol unit, the microcontrol unit outputs a dimming signal for dimming, and the connector is detachably connected with a connector of another device.

Abstract: A controller (306) executes a method (400) of controlling a solar power converter (20, 300) connected to receive output power from a solar power source (10). The method comprises: measuring (430) an open circuit voltage (VOC) of the solar power source; determining a short circuit current (ISC) output by the solar power source; using (440) the measured open circuit voltage (VOC) and the measured short circuit current (ISC) to determine an estimate of a voltage maximum power point (VMPP) for the solar power source corresponding to a maximum power point (MPP) for transferring the output power from the solar power source to a load; executing (450) a perturb-and-observe algorithm (500) beginning at the estimated VMPP to determine an actual VMPP for transferring the output power from the solar power source to the load; and operating (470) the solar power converter at or approximately at the actual VMPP.