Abstract: Methods for isolating K+Hnov genes are provided. The K+Hnov nucleic acid compositions find use in identifying homologous or related proteins and the DNA sequences encoding such proteins; in producing compositions that modulate the expression or function of the protein; and in studying associated physiological pathways. In addition, modulation of the gene activity in vivo is used for prophylactic and therapeutic purposes, such as identification of cell type based on expression, and the like.

Abstract: Nucleic acid hybridization under steady-state conditions is described by a kinetic model in which the intermediate state is assumed to be locally single stranded. An expression was derived that relates nucleic acid secondary structure to the rate of oligonucleotide-RNA hybridization. The model allows the calculation of a rate factor that is proportional to the rate constant for hybridization between complementary nucleic acids and is generally applicable to any RNA molecule with potential utility for rapid identification of sites for antisense attack of mRNA.

Abstract: Nucleic acid hybridization under steady-state conditions is described by a kinetic model in which the intermediate state is assumed to be locally single stranded. An expression was derived that relates nucleic acid secondary structure to the rate of oligonucleotide-RNA hybridization. The model assumes that the hybridization of nucleic acids occurs through an intermediate state in which the region to be hybridized has a single-stranded conformation prior to binding the antisense oligonucleotide. In the derivation, a steady-state condition is assumed. The model is applicable to the steady-state condition in living cells and the initial stage of single-tube hybridization when full-length hybrid has not significantly accumulated and the concentration of nucleation complex is approximately constant. The model allows the calculation of a rate factor that is proportional to the rate constant for hybridization between complementary nucleic acids.