Abstract: The disclosure relates to a control method for an automated guided forklift, and an automated guided forklift and a controller applying same. The method includes the following steps: determining an angle and a height of the body of the automated guided forklift; determining a height of a fork hole of the carrier; and adjusting an angle and vertical displacement of a fork of the automated guided forklift according to the height of the fork hole and the angle and the height of the body, so as to limit an included angle between the fork and the carrier within a first angle threshold range, and to limit a height difference between prongs of the fork and the fork hole within a first distance threshold range.

Abstract: Disclosed are an aligning method, a controller, and material handling equipment. A major technical solution includes: acquiring target data of a first stacking object and a second stacking object by using a first sensor; extracting, from the target data, first target data of the first stacking object and second target data of the second stacked object; calculating a relative pose of the first stacking object relative to the second stacking object based on the first target data and the second target data; and controlling, based on the relative pose, material handling equipment to move, to align the first stacking object with the second stacking object. In the present disclosure, a relative pose between two stacking objects is corrected by simultaneously acquiring and processing target data of the two stacking objects, thereby significantly improving accuracy and efficiency of a stacking operation.

Abstract: Disclosed are an aligning method, a controller, and material handling equipment. A major technical solution includes: acquiring target data of a first stacking object and a second stacking object by using a first sensor; extracting, from the target data, first target data of the first stacking object and second target data of the second stacked object; calculating a relative pose of the first stacking object relative to the second stacking object based on the first target data and the second target data; and controlling, based on the relative pose, material handling equipment to move, to align the first stacking object with the second stacking object. In the present disclosure, a relative pose between two stacking objects is corrected by simultaneously acquiring and processing target data of the two stacking objects, thereby significantly improving accuracy and efficiency of a stacking operation.

Abstract: Disclosed are an aligning method, a controller, and material handling equipment. A major technical solution includes: acquiring target data of a first stacking object and a second stacking object by using a first sensor; extracting, from the target data, first target data of the first stacking object and second target data of the second stacked object; calculating a relative pose of the first stacking object relative to the second stacking object based on the first target data and the second target data; and controlling, based on the relative pose, material handling equipment to move, to align the first stacking object with the second stacking object. In the present disclosure, a relative pose between two stacking objects is corrected by simultaneously acquiring and processing target data of the two stacking objects, thereby significantly improving accuracy and efficiency of a stacking operation.

Abstract: Provided herein are genetically engineered T cells containing a chimeric antigen receptor (CARs), and related methods and uses thereof in allogeneic cell therapy. In some embodiments, the T cells are genetically engineered with a CAR and are further genetically engineered by one or more strategies to reduce host immune recognition of the engineered T cells, such as by heterologous expression of one or more additional transgenes and by genetic disruption to reduce or eliminate expression or one or more endogenous protein. Also provided are cell compositions containing the engineered T cells, and related methods, kits and systems for producing the engineered T cells. Also provided are methods of making and using the engineered T cells for cell therapy, including in connection with cancer immunotherapy comprising adoptive transfer of the engineered T cells.

Abstract: An automated guided forklift and a fork control method therefor are provided. The automated guided forklift includes a fork and a controller. The controller is configured to perform the following steps: receiving a transverse shift step size, a rotation step size, and a target direction of the fork; controlling the fork to perform a transverse shift motion based on the transverse shift step size and the target direction; and controlling the fork to perform a rotation motion based on the rotation step size and the target direction.

Abstract: The present invention provides a degradable microbead comprising a polymer molecule crosslinked by a crosslinking agent, wherein the polymer molecule and/or the crosslinking agent comprises a sensitive chemical bond that is cleavable through a chemical and/or light treatment, thereby resulting in the degradation of the degradable microbead. The present invention also provides a method of separating a target protein from a sample. By using the degradation of the degradable microbead to replace an elution step in protein purification, it is possible to select a combination of target protein and affinity ligand with a stronger affinity, thereby improving the protein purification efficiency. The method is especially suitable for the high-throughput preparation of multiple protein samples, for example providing a protein sample for electron microscope observation or mass spectrometry measurement.

Abstract: The present disclosure provides systems, methods, and compositions for conditionally regulating expression of a target gene. Aspects of the present disclosure utilize intracellular signal transduction pathways to regulate the expression of a gene (e.g., transgene, exogenous gene, endogenous gene).

Abstract: A processor and an operating method thereof for renaming a destination logical register of a move instruction are provided. The processor comprises a plurality of physical registers and a renaming circuit. The renaming circuit is coupled to the plurality of physical registers and is configured to receive an instruction sequence and check the instruction sequence. When a current instruction of the instruction sequence comprises the move instruction, the renaming circuit assigns a first physical register, which is assigned to a source logical register of the current instruction previously, to the destination logical register of the current instruction. The first physical register is one of the plurality of physical registers.

Abstract: A cache memory device is provided in the disclosure. The cache memory device includes a first AGC, a compression circuit, a second AGC, a virtual tag array, and a comparator circuit. The first AGC generates a virtual address based on a load instruction. The compression circuit obtains the higher part of the virtual address and generates a target hash value based on the higher part of the virtual address. The second AGC generates the lower part of the virtual address based on the load instruction. The virtual tag array obtains the lower part and selects a set of memory units. The comparator circuit compares the target hash value to a hash value stored in each memory unit of the set of memory units. When the comparator circuit generates the virtual tag miss signal, the comparator circuit transmits the virtual tag miss signal to the reservation station.

Abstract: A cache memory device is provided in the disclosure. The cache memory device includes a first AGC, a compression circuit, a second AGC, a virtual tag array, and a comparator circuit. The first AGC generates a virtual address based on a load instruction. The compression circuit obtains the higher part of the virtual address and generates a target hash value based on the higher part of the virtual address. The second AGC generates the lower part of the virtual address based on the load instruction. The virtual tag array obtains the lower part and selects a set of memory units. The comparator circuit compares the target hash value to a hash value stored in each memory unit of the set of memory units. When the comparator circuit generates the virtual tag miss signal, the comparator circuit transmits the virtual tag miss signal to the reservation station.

Abstract: Disclosed herein are methods and compositions for generating a single-stranded break in a target sequence, which facilitates targeted integration of one or more exogenous sequences.

Abstract: An instruction execution method suitable for being executed by a processor is provided. The first processor comprises a register alias table (RAT) and a reservation station. The instruction execution method includes: a register alias table receives a first micro-instruction and a second micro-instruction and issues the first micro-instruction and the second micro-instruction to the reservation station; and the reservation station assigns one of a plurality of execution units to execute the first micro-instruction, according to the first specific message of the first micro-instruction; and the reservation station assigns one of the execution units to execute the second micro-instruction, according to the second specific message of the second micro-instruction.

Abstract: A processor and an operating method thereof are provided. The processor comprises a plurality of physical registers and a renaming circuit. The renaming circuit is coupled to the plurality of physical registers and is configured to receive an instruction sequence and check the instruction sequence. When a current instruction of the instruction sequence comprises a move instruction, the renaming circuit assigns a first physical register, which is assigned to a source logical register of the current instruction previously, to a destination logical register of the current instruction. The first physical register is one of the plurality of physical registers.

Abstract: Disclosed herein are methods and compositions for generating a single-stranded break in a target sequence, which facilitates targeted integration of one or more exogenous sequences.

Abstract: The present invention provides a degradable microbead comprising a polymer molecule crosslinked by a crosslinking agent, wherein the polymer molecule and/or the crosslinking agent comprises a sensitive chemical bond that is cleavable through a chemical and/or light treatment, thereby resulting in the degradation of the degradable microbead. The present invention also provides a method of separating a target protein from a sample. By using the degradation of the degradable microbead to replace an elution step in protein purification, it is possible to select a combination of target protein and affinity ligand with a stronger affinity, thereby improving the protein purification efficiency. The method is especially suitable for the high-throughput preparation of multiple protein samples, for example providing a protein sample for electron microscope observation or mass spectrometry measurement.

Abstract: The present invention relates to a method of reducing the production of glutamate from glutamine by glutaminase C in a cell or tissue. The method involves inhibiting glutaminase C activity in the cell or tissue under conditions effective to reduce production of glutamate from glutamine. Compounds for carrying out this method are also disclosed.

Abstract: The present disclosure provides a system for regulating expression of a target polynucleotide in a cell. The system may comprise a chimeric polypeptide comprising a gene modulating polypeptide fused in-frame with a heterologous nuclear localization domain. The heterologous nuclear localization domain may be operable to translocate the chimeric polypeptide to a cell nucleus upon activation by an active cellular signaling pathway. The cellular signaling pathway may be inducible in response to an extracellular signal. In response to the extracellular signal, the chimeric polypeptide may localize to the cell nucleus and the gene modulating polypeptide may regulate expression of a target polynucleotide in the cell nucleus.