Abstract: The present disclosure provides a video generation method, an apparatus, a system, a device and a storage medium. The method is applied to a video generation system, and the video generation system includes a first client and at least one second client. Specifically, the method includes: firstly, the first client creates a first virtual room and sets a target capturing effect for the first virtual room; then, the second client acquires a first video added with the target capturing effect after receiving a notification message that a current user has successfully joined the first virtual room, and uploads the first video to the first virtual room. Further, the first client acquires a target video from the first virtual room and generates a target result video based on the target video, and the target video includes the first video.

Abstract: Disclosed are methods of message processing, apparatuses therefor, and an electronic device. An implementation of the disclosure includes: presenting, responsive to a user's preset trigger operation in a target conversation, a schedule set area and a scheduled send button in the target conversation; determining a message send time set by the user via the schedule set area; and retrieving, after the user triggers the scheduled send button, message content in a message input box of the target conversation as message content for a target scheduled message, and creating the target scheduled message according to the message send time. This implementation realizes direct creation of a scheduled message via a message input box of an instant messaging client, facilitates converting an edited instant message to a scheduled message, extends functions of the instant messaging client, and enhances user experience.

Abstract: The present invention provides a fermentation process for improving the production level of a recombinant human collagen. The process comprises inoculating a sterilized fermentation medium with a Pichia yeast solution, then performing fermentation culturing for 14-18 hrs, adding methanol to induce the expression, upon which sodium pyruvate is added to the fermentation medium, wherein the amount of the sodium pyruvate added is 0.01-10 g/L. In the fermentation process of the present invention, the sodium pyruvate is added in the methanol-induced expression stage, so that the biosynthesis rate of the recombinant human collagen is improved; and a continuous feed-batch mode is adopted, so that the biosynthesis rate of the recombinant human collagen is further improved, and the fermentation time is shortened. Meanwhile, the expression level of the recombinant human collagen is increased, the fermentation level is increased by more than 20%, and the production cost is reduced.

Abstract: The present invention discloses a fermentation process with a Pichia yeast expressing a recombinant protein, in which Pichia pastoris is used as a fungal strain. The process comprises performing primary seed culturing to reach a fungal concentration of 20±2 g/L; then performing secondary seed culturing to reach a fungal concentration of 120±10 g/L; next proceeding to a glycerol culturing stage, wherein the amount of glycerol added in the glycerol culturing stage is 60-70 g/L; and then proceeding to a methanol-induced stage for 120±8 h after the dissolved oxygen quickly reaches a relatively stable state, to complete the fermentation process. In the present invention, a glycerol fed-batch addition stage in existing processes is omitted, and the process proceeds to a next stage as soon as glycerol is completely consumed, with no need to prepare sterilized glycerol for fluidic addition. As such, the glycerin sterilizer is omitted, the consumption of energy and resource and the waste of glycerin are reduced.

Abstract: The present invention discloses a fermentation process with a Pichia yeast expressing a recombinant protein, in which Pichia pastoris is used as a fungal strain. The process comprises performing primary seed culturing to reach a fungal concentration of 20±2 g/L; then performing secondary seed culturing to reach a fungal concentration of 120±10 g/L; next proceeding to a glycerol culturing stage, wherein the amount of glycerol added in the glycerol culturing stage is 60-70 g/L; and then proceeding to a methanol-induced stage for 120±8 h after the dissolved oxygen quickly reaches a relatively stable state, to complete the fermentation process. In the present invention, a glycerol fed-batch addition stage in existing processes is omitted, and the process proceeds to a next stage as soon as glycerol is completely consumed, with no need to prepare sterilized glycerol for fluidic addition. As such, the glycerin sterilizer is omitted, the consumption of energy and resource and the waste of glycerin are reduced.

Abstract: The present invention provides a fermentation process for improving the production level of a recombinant human collagen. The process comprises inoculating a sterilized fermentation medium with a Pichia yeast solution, then performing fermentation culturing for 14-18 hrs, adding methanol to induce the expression, upon which sodium pyruvate is added to the fermentation medium, wherein the amount of the sodium pyruvate added is 0.01-10 g/L. In the fermentation process of the present invention, the sodium pyruvate is added in the methanol-induced expression stage, so that the biosynthesis rate of the recombinant human collagen is improved; and a continuous feed-batch mode is adopted, so that the biosynthesis rate of the recombinant human collagen is further improved, and the fermentation time is shortened. Meanwhile, the expression level of the recombinant human collagen is increased, the fermentation level is increased by more than 20%, and the production cost is reduced.

Abstract: Disclosed in the present invention is a four-port power electronic transformer based on a hybrid modular multilevel converter (MMCs). The four-port power electronic transformer includes a hybrid MMC, direct current (DC)/DC converters, and an inverter. Each DC/DC converter includes a front stage part, a high frequency transformation part and a back stage part.

Abstract: Disclosed in the present invention is a four-port power electronic transformer based on a hybrid modular multilevel converter (MMCs). The four-port power electronic transformer includes a hybrid MMC, direct current (DC)/DC converters, and an inverter. Each DC/DC converter includes a front stage part, a high frequency transformation part and a back stage part.

Abstract: A method of data transmission between power electronic devices without a communication line involves generation of a digital signal in a data transmission process. The digital signal enables PWM modulation of specific data information via a composite modulation method. The composite modulation superimposes PWM modulation waves after modulating the PWM carrier frequency or conducting high-frequency modulation on the data. The composite modulation generates a PWM drive pulse signal which is transmitted to a power circuit via a main power electronic circuit to complete the data transmission process. In the data receiving process, signals are extracted on the voltage and current of a power line via software or hardware, and data demodulated to obtain the data information to complete the data receiving process.

Abstract: A method of data transmission between power electronic devices without a communication line involves generation of a digital signal in a data transmission process. The digital signal enables PWM modulation of specific data information via a composite modulation method. The composite modulation superimposes PWM modulation waves after modulating the PWM carrier frequency or conducting high-frequency modulation on the data. The composite modulation generates a PWM drive pulse signal which is transmitted to a power circuit via a main power electronic circuit to complete the data transmission process. In the data receiving process, signals are extracted on the voltage and current of a power line via software or hardware, and data demodulated to obtain the data information to complete the data receiving process.