Patents by Inventor Jiangping Lu

Jiangping Lu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12098394
    Abstract: An amino acid sequence of the monooxygenase mutant is obtained by mutation of an amino acid sequence shown in SEQ ID NO: 1, and the mutation at least includes one of the following mutation sites: 45-th site, 95-th site, 106-th site, 108-th site, 114-th site, 186-th site, 190-th site, 191-th site, 249-th site, 257-th site, 393-th site, 436-th site, 499-th site, 500-th site, 501-th site, 503-th site, 504-th site, 559-th site, and 560-th site.
    Type: Grant
    Filed: November 5, 2018
    Date of Patent: September 24, 2024
    Assignee: ASYMCHEM LABORATORIES (TIANJIN) CO., LTD.
    Inventors: Hao Hong, Gage James, Jiangping Lu, Xuecheng Jiao, Na Zhang, Kejian Zhang, Rui Li, Yu Zhang, Yiming Yang
  • Patent number: 12084402
    Abstract: Provided is a preparation process of diazomethane. The preparation process includes: step S1, taking N-methylurea as a raw material to continuously prepare, in a continuous reactor, a first product system containing N-methyl-N-nitrosourea: step S2, performing continuous extraction and continuous back-extraction on the first product system to obtain an N-methyl-N-nitrosourea solution; step S3, enabling the N-methyl-N-nitrosourea solution to continuously react with an alkaline solution in a continuous reactor to obtain a second product system containing the diazomethane; and step S4, performing continuously liquid separation, water freezing and removal on the second product system, to obtain the diazomethane.
    Type: Grant
    Filed: May 21, 2019
    Date of Patent: September 10, 2024
    Assignee: ASYMCHEM LABORATORIES (TIANJIN) CO., LTD.
    Inventors: Hao Hong, Jiangping Lu, Enxuan Zhang, Wei Shen, Honglei Yan, Zhen Zhang
  • Patent number: 11993552
    Abstract: The present disclosure discloses a method and a device for continuously synthesizing cyclopropane compounds. The method includes the following steps: continuously performing a synthetic reaction of a diazomethane precursor in a first reactor, the reaction product of the first reactor flowing into a separator for stratification, the organic phase obtained by stratification overflowing into a second reactor, continuously consuming the diazomethane precursor in a second reactor to prepare diazomethane and performing an electron-rich monoolefin cyclopropanation reaction in situ so as to obtain the cyclopropane compound.
    Type: Grant
    Filed: July 2, 2019
    Date of Patent: May 28, 2024
    Assignee: ASYMCHEM LABORATORIES (TIANJIN) CO., LTD.
    Inventors: Hao Hong, Jiangping Lu, Xichun Feng, Xingfang Sun, Feng Zhang
  • Patent number: 11952575
    Abstract: A transaminase mutant and use hereof, the amino acid sequence of the transaminase mutant is an amino acid sequence in which the amino acid sequence as represented by SEQ ID NO: 1 is mutated, the mutated amino acid position being one or more selected from among F89, K193, P243, V234, I262, Q280, V379, R416, A417 and C418. The enzymatic activity and/or stability of the transaminase mutant is improved.
    Type: Grant
    Filed: November 15, 2017
    Date of Patent: April 9, 2024
    Assignee: ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD
    Inventors: Hao Hong, Gage James, Jiangping Lu, Xingfu Xu, Wenyan Yu, Xin Huang, Yulei Ma, Yibing Cheng
  • Patent number: 11858873
    Abstract: Disclosed is a method for continuously synthesizing a propellane compound. The method includes the following steps: using 1,1-dibromo-2,2-bis(chloromethyl)cyclopropane or a derivative thereof as a raw material to form a ring with a lithium metal agent by a continuous reaction, so as to synthesize the propellane compound. A technical scheme of the present disclosure is applied, and a continuous reaction device is used.
    Type: Grant
    Filed: June 18, 2019
    Date of Patent: January 2, 2024
    Assignee: JILIN ASYMCHEM LABORATORIES CO., LTD.
    Inventors: Hao Hong, Enxuan Zhang, Jiangping Lu, Fuliang Wei, Sihang Yang
  • Patent number: 11827909
    Abstract: Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises having the amino acid sequence of SEQ ID NO: 2 with the exception of a mutation of one or more amino acids; wherein the mutation of one or more amino acids must comprises E27K, and the mutation of one or more amino acids selected from the group consisting of: H14R, L16N, T25R, F26L, E27K, D30S, S33N, E34N, E34G, E34L, E34S, E34D, Y35W, Y35K, S37W, S37F, S37E, S37N, S37T, S37C, W40F, K41E, D54G, H55Q, S57L, I58T, I58Y, I58A, I58R, I58V, I58S, I58C, K86P, T91A, F95Y, C97Y, I98V, K106V, K106T, K106Q, F111S, K112E, K112R, S154A, K162E, L166M, I118F, I118V, I118R, H119R, H119F, I120V, K123D, K123N, K123Q, K123S, K123I, K123T, T130N, D134G, V135K, N165H, D173G, K209R, I223V and S225A, and having proline hydroxylase activity.
    Type: Grant
    Filed: December 10, 2020
    Date of Patent: November 28, 2023
    Assignees: ASYMCHEM LABORATORIES (TIANJIN) CO., LTD., ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD., TIANJIN ASYMCHEM PHARMACEUTICALS CO., LTD., ASYMCHEM LABORATORIES (FUXIN) CO., LTD., JILIN ASYMCHEM LABORATORIES CO., LTD.
    Inventors: Hao Hong, Gage James, Jiangping Lu, Na Zhang, Wenyan Yu, Fang Liu, Yanjun Li, Xin Huang, Juan Gao, Kejian Zhang, Yulei Ma, Junlu Wei
  • Patent number: 11702640
    Abstract: The present application relates to the technical field of genetic engineering, and provides a monooxygenase mutant, a preparation method and application thereof. The monooxygenase mutant has any one of the amino acid sequences shown in (I) and (II): (I) an amino acid sequence having at least 80% identity with the amino acid sequence shown in SEQ ID NO. 1; and (II) an amino acid sequence obtained by modifying, substituting, deleting, or adding one or several amino acids to the amino acids at 23 to 508 positions of the amino acid sequence shown in SEQ ID NO. 1, the substituting referring to a substitution of 1 to 34 amino acids, wherein the mutant has the activity of monooxygenase.
    Type: Grant
    Filed: February 8, 2018
    Date of Patent: July 18, 2023
    Assignee: ASYMCHEM LABORATORIES (TIANJIN) CO., LTD.
    Inventors: Hao Hong, James Gage, Jiangping Lu, Xuecheng Jiao, Na Zhang, Rui Li, Kejian Zhang, Yu Zhang
  • Patent number: 11603521
    Abstract: Provided is an amino acid dehydrogenase mutant. The amino acid sequence of the mutant is obtained by mutating the amino acid sequence shown in SEQ ID NO:1. The mutation includes at least one of the following mutation sites: 64th, 94th, 133rd, 137th, 148th, 168th, 173rd, 183 rd, 191st, 207th, 229th, 248th, 255th and 282nd sites; or the amino acid sequence of the amino acid dehydrogenase mutant is an amino acid sequence having the mutation sites in the mutated amino acid sequence and having a 80% or more homology with the mutated amino acid sequence. The mutant enzyme activity is more than 50 times higher than that of wild amino acid dehydrogenase, and the enzyme specificity is also correspondingly improved.
    Type: Grant
    Filed: August 17, 2018
    Date of Patent: March 14, 2023
    Assignee: ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD
    Inventors: Hao Hong, Gage James, Jiangping Lu, Na Zhang, Xuecheng Jiao, Rui Li, Kejian Zhang, Yu Zhang, Yiming Yang
  • Publication number: 20220380292
    Abstract: A preparation method for a trans-cyclobutane-o-dicarboxylic acid ester and a derivative thereof includes the following steps: in an organic solvent, catalyzing a substrate with a structure as shown in a structural formula I by using organic alkali at 50-90° C. so as to generate isomerization, acquiring the trans-cyclobutane-o-dicarboxylic acid ester or the derivative thereof, herein the structural formula I is as follows: Each R1, R2, R3 and R4 is independently one of hydrogen or an alkyl of C1-C5, and each of R5 and R6 is independently one of alkyl of C1-C10 and benzyl.
    Type: Application
    Filed: October 25, 2019
    Publication date: December 1, 2022
    Inventors: Hao HONG, Enxuan ZHANG, Jiangping LU, Fuliang WEI, Guanda CHE, Mingjie FENG
  • Publication number: 20220380273
    Abstract: Disclosed is a method for continuously synthesizing a propellane compound. The method includes the following steps: using 1,1-dibromo-2,2-bis(chloromethyl)cyclopropane or a derivative thereof as a raw material to form a ring with a lithium metal agent by a continuous reaction, so as to synthesize the propellane compound. A technical scheme of the present disclosure is applied, and a continuous reaction device is used.
    Type: Application
    Filed: June 18, 2019
    Publication date: December 1, 2022
    Inventors: Hao HONG, Enxuan ZHANG, Jiangping LU, Fuliang WEI, Sihang YANG
  • Publication number: 20220380280
    Abstract: The disclosure discloses a method for an addition reaction of acetylene and a ketone compound. The method includes the following steps: S1, providing a continuous reaction device, wherein the continuous reaction device includes a plurality of bubble tubular reactors being connected with each other through connecting tubes; feeding a raw material solution containing the ketone compound and alkali into the plurality of bubble tubular reactors, and S3, under normal pressure, pumping acetylene from the bottom of the first bubble tubular reactor for the addition reaction. By applying the technical solution of the invention, acetylene reacts with the ketone compound in the plurality of bubble tubular reactors arranged in series, which can ensure the sufficient gas-liquid contact time, and thus making full use of the acetylene gas, improving the utilization rate thereof, effectively reduing the amount of acetylene, reducing costs, and further improving the safety.
    Type: Application
    Filed: September 17, 2019
    Publication date: December 1, 2022
    Inventors: Hao HONG, Jiangping LU, Xichun FENG, Xin ZHANG, Bo YAN
  • Publication number: 20220340517
    Abstract: Provided is a continuous synthesis method for ethoxymethylenemalononitrile. The method includes the following steps: malononitrile, triethyl orthoformate and acetic anhydride are continuously fed into a continuous reaction device to perform a condensation reaction, to obtain the ethoxymethylenemalononitrile, and in the process of the condensation reaction, the generated ethoxymethylenemalononitrile is continuously discharged; herein, the molar ratio of the malononitrile, the triethyl orthoformate and the acetic anhydride is 1:(0.9-6.0):(2.0-6.0). By adopting the continuous reaction device in the present disclosure, since the amount of materials involved in the reaction per unit time is greatly reduced, a high temperature dangerous area is reduced, and a safety risk is greatly reduced.
    Type: Application
    Filed: December 18, 2019
    Publication date: October 27, 2022
    Inventors: Hao HONG, Jiangping LU, Denghui BAO, Chaojie LIU, Xiaotong JIA
  • Publication number: 20220332732
    Abstract: The application provides a continuous preparation method for benzylzinc halide and a derivative thereof.
    Type: Application
    Filed: September 24, 2019
    Publication date: October 20, 2022
    Inventors: Hao HONG, Jiangping LU, Xichun FENG, Xingfang SUN, Yong LIANG
  • Publication number: 20220315615
    Abstract: The present disclosure discloses a continuous preparation method for a penem intermediate MAP. The continuous preparation method includes the following steps: step S1, in a column-type continuous reactor, using a rhodium-loaded catalyst to catalyze 4-nitrobenzyl(R)-2-diazo-4-((2R,3S)-3-((R)-1-hydroxyethyl)-4-oxoazetidin-2-yl)-3-oxopentanoate to generate a cyclization reaction so as to form a first intermediate, herein the rhodium-loaded catalyst is loaded in the column-type continuous reactor, and the rhodium-loaded catalyst has the following structural formula: step S2, performing an esterification reaction on the first intermediate, a diphenyl chlorophosphate and a diisopropylethylamine in a second continuous reactor, to obtain a product system containing the penem intermediate MAP; and step S3, performing crystallization treatment on the product system, to obtain the penem intermediate MAP.
    Type: Application
    Filed: October 28, 2019
    Publication date: October 6, 2022
    Inventors: Hao HONG, Gage JAMES, Chaoyong CHEN, Jiangping LU, Yong JIANG, Jian TAO, Yong LUO
  • Publication number: 20220297080
    Abstract: The present disclosure provides a device for continuously preparing 2,6-dihydroxybenzaldehyde and use thereof. The device includes a first continuous reaction unit for hydroxy protection reaction, a second continuous reaction unit for lithiation and hydroformylation, and a third continuous reaction unit for deprotection reaction that are connected in series. The third continuous reaction unit includes: a first columnar continuous reactor, connected to the second continuous reaction unit and used for deprotection of the lithiated hydroformylated product while performing liquid separation to obtain an organic phase containing 2,6-dihydroxybenzaldehyde and an aqueous phase. When the device is applied in the preparation of 2,6-dihydroxybenzaldehyde, reaction time is shortened and the intermediate purification treatment is no longer required.
    Type: Application
    Filed: September 12, 2019
    Publication date: September 22, 2022
    Inventors: Hao HONG, Jiangping LU, Xichun FENG, Xin ZHANG, Bo YAN
  • Publication number: 20220259122
    Abstract: Disclosed are a borohydride reduction stabilizing system and a method for reducing an ester to an alcohol. The borohydride reduction stabilizing system includes: a borohydride reducing agent and a stabilizing agent for stabilizing the borohydride reducing agent. The borohydride reducing agent is sodium borohydride or potassium borohydride. The stabilizing agent is an alkali metal salt of an alcohol. By adding the alkali metal salt of the alcohol, such as sodium alkoxide or potassium alkoxide, on the basis of an existing sodium/potassium borohydride reducing agent, the sodium/potassium borohydride reducing agent may be kept stable without being decomposed under the condition of increased temperature, so that on the one hand, the reducing activity is maintained in a relatively high state, and the condition of excessive use is reduced, and on the other hand, the generation of hydrogen is reduced, and the process risks are reduced.
    Type: Application
    Filed: June 11, 2019
    Publication date: August 18, 2022
    Inventors: Hao HONG, Jiangping LU, Enxuan ZHANG, Zhiqing LIU, Tao ZHANG
  • Publication number: 20220251034
    Abstract: The present disclosure provides a preparation method for metformin hydrochloride. The preparation method includes: microwave heating raw materials containing dicyandiamide and dimethylamine hydrochloride, and reacting the two at 100° C. to 160° C. to obtain a product system containing metformin hydrochloride. Microwave heating is adopted to heat the dicyandiamide and the dimethylamine hydrochloride. Compared with a conventional heating mode that requires heat to be gradually transferred from the outside to the inside, microwave heating can directly heat each part inside the reactant, which can make the internal temperature of the reactant more uniform, thereby reducing the generation of impurities.
    Type: Application
    Filed: July 25, 2019
    Publication date: August 11, 2022
    Inventors: Hao HONG, Jiangping LU, Jian TAO, Yan ZHANG, Fan WANG, Gonzalez MIGUEL
  • Patent number: 11407982
    Abstract: Disclosed are transaminase mutants and use thereof. The amino acid sequence of the transaminase mutant is obtained by the mutation of the amino acid sequence as shown in SEQ ID NO: 1, and the mutation at least comprises one of the following mutation sites: the 19-th site, the 41-th site, the 43-th site, the 72-th site, the 76-th site, the 92-th site, the 107-th site, the 125-th site, the 132-th site, the 226-th site, the 292-th site, the 295-th site, the 308-th site, and the 332-th site; and the 19-th site is mutated into a serine, the 41-th site is mutated into a serine, the 43-th site is mutated into an asparagine, a glycine in the 72-th site is mutated into a leucine, etc.; or the amino acid sequence of the transaminase mutant has the mutation sites in the mutanted amino acid sequence, and has more than 80% homology to the mutanted amino acid sequence.
    Type: Grant
    Filed: November 6, 2017
    Date of Patent: August 9, 2022
    Assignee: ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD
    Inventors: Hao Hong, Gage James, Jiangping Lu, Xuecheng Jiao, Na Zhang, Rui Li, Kejian Zhang
  • Publication number: 20220242818
    Abstract: Provided is a preparation process of diazomethane. The preparation process includes: step S1, taking N-methylurea as a raw material to continuously prepare, in a continuous reactor, a first product system containing N-methyl-N-nitrosourea; step S2, performing continuous extraction and continuous back-extraction on the first product system to obtain an N-methyl-N-nitrosourea solution; step S3, enabling the N-methyl-N-nitrosourea solution to continuously react with an alkaline solution in a continuous reactor to obtain a second product system containing the diazomethane; and step S4, performing continuously liquid separation, water freezing and removal on the second product system, to obtain the diazomethane.
    Type: Application
    Filed: May 21, 2019
    Publication date: August 4, 2022
    Inventors: Hao HONG, Jiangping LU, Enxuan ZHANG, Wei SHEN, Honglei YAN, Zhen ZHANG
  • Publication number: 20220242805
    Abstract: The present disclosure discloses a method and a device for continuously synthesizing cyclopropane compounds. The method includes the following steps: continuously performing a synthetic reaction of a diazomethane precursor in a first reactor, the reaction product of the first reactor flowing into a separator for stratification, the organic phase obtained by stratification overflowing into a second reactor, continuously consuming the diazomethane precursor in a second reactor to prepare diazomethane and performing an electron-rich monoolefin cyclopropanation reaction in situ so as to obtain the cyclopropane compound. The technical solution of the present disclosure may be applied to achieve automatic control, decrease the transfer of high-risk materials, and prevent the risk of pipeline transfer of diazomethane solution, and effectively improve production safety.
    Type: Application
    Filed: July 2, 2019
    Publication date: August 4, 2022
    Inventors: Hao HONG, Jiangping LU, Xichun FENG, Xingfang SUN, Feng ZHANG