Abstract: An electrochemical biosensor for detection of a calprotectin antigen in a sample solution. The electrochemical biosensor includes a reference electrode, a counter electrode, and a working electrode. The working electrode includes a surface coated with an anti-calprotectin antibody that binds to the calprotectin antigen in the sample solution. The electrochemical biosensor detects the concentration of the calprotectin antigen in the sample solution based on a resistance change at the surface of the working electrode.

Abstract: Passive devices for non-invasive gastrointestinal (GI) sampling are disclosed. In some embodiments, a device includes a capsule housing containing an absorbent sampling hydrogel. The capsule may be ingested by a patient and subsequently travel through the GI tract. Once the capsule reaches a desired location in the GI tract, GI fluid may flow into the capsule through a sampling aperture of the capsule. The fluid may be absorbed by and stored within the sampling hydrogel for subsequent analysis, and the absorption of the fluid may cause the sampling hydrogel to expand within the capsule. The capsule may further include a sealing member positioned between the sampling hydrogel and the sampling aperture, and the expansion of the sampling hydrogel may press the sealing member against the sampling aperture to seal the capsule.

Abstract: The present disclosure is drawn to compositions, methods, and systems for loop-mediated isothermal amplification (LAMP) analysis on a solid phase medium. The composition can comprise one or more target primers, a DNA polymerase, and a re-solubilization agent. The composition can be substantially free of non-pH sensitive agents capable of discoloring the solid phase medium. The method can comprise providing an assembly of a solid phase medium, depositing a biological sample onto the solid phase medium, and heating the assembly to an isothermal temperature sufficient to facilitate a LAMP reaction. The system can comprise a composition and a solid phase medium on to which the composition is deposited.

Abstract: The present disclosure is drawn to loop-mediated isothermal amplification (LAMP) reaction assemblies including a substantially hygroscopic agent free LAMP reagent mixture in combination with a solid-phase reaction medium. The present disclosure also includes systems for a chromatic LAMP analysis including a substantially non-reactive solid phase reaction medium, and a non-interfering reagent mixture. The present disclosure also includes solid phase LAMP reaction mediums comprising a substrate, an adhesive layer disposed on the substrate, a reaction layer disposed on the adhesive layer, and a spreading layer disposed on the reaction layer. The present disclosure also includes methods of testing for a presence of a target nucleotide sequence including providing a biological sample, and dispensing the sample into a test environment having a solid phase reaction medium in combination with a LAMP reagent mixture and a pH sensitive dye.

Abstract: The present disclosure is drawn to loop-mediated isothermal amplification (LAMP) reaction assemblies including a substantially hygroscopic agent free LAMP reagent mixture in combination with a solid-phase reaction medium. The present disclosure also includes systems for a chromatic LAMP analysis including a substantially non-reactive solid phase reaction medium, and a non-interfering reagent mixture. The present disclosure also includes solid phase LAMP reaction mediums comprising a substrate, an adhesive layer disposed on the substrate, a reaction layer disposed on the adhesive layer, and a spreading layer disposed on the reaction layer. The present disclosure also includes methods of testing for a presence of a target nucleotide sequence including providing a biological sample, and dispensing the sample into a test environment having a solid phase reaction medium in combination with a LAMP reagent mixture and a pH sensitive dye.

Abstract: The present disclosure is drawn to loop-mediated isothermal amplification (LAMP) reaction assemblies including a substantially hygroscopic agent free LAMP reagent mixture in combination with a solid-phase reaction medium. The present disclosure also includes systems for a chromatic LAMP analysis including a substantially non-reactive solid phase reaction medium, and a non-interfering reagent mixture. The present disclosure also includes solid phase LAMP reaction mediums comprising a substrate, an adhesive layer disposed on the substrate, a reaction layer disposed on the adhesive layer, and a spreading layer disposed on the reaction layer. The present disclosure also includes methods of testing for a presence of a target nucleotide sequence including providing a biological sample, and dispensing the sample into a test environment having a solid phase reaction medium in combination with a LAMP reagent mixture and a pH sensitive dye.

Abstract: The present disclosure is drawn to an isolated complementary DNA (cDNA) of a nucleic acid molecule that can comprise a nucleotide sequence that is at least 85% identical to SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, or a combination thereof. In one embodiment, a primer set for reverse transcription loop-mediated isothermal amplification (RT-LAMP) analysis can comprise a forward inner primer (FIP) sequence, a backward inner primer (BIP) sequence, a forward outer primer (F3) sequence, a backward outer primer (B3) sequence, a forward loop primer (LF) sequence, and a backward loop primer (LB) sequence. In another embodiment, a method of detecting a target pathogen can comprise providing a primer set.

Abstract: A technology is described for a system for identifying a colorimetric test result from a pathogen test performed on a solid phase substrate. The system can comprise a sensor configured to detect a spectrum of color wavelengths. The system can comprise one or more processors. The one or more processors can be configured to: receive color wavelength data; determine a wavelength threshold for providing a pathogen positive test result; identify whether the color wavelength data meets or exceeds the wavelength threshold for providing a pathogen positive test result; and generate a result indicator indicating either a pathogen positive or pathogen negative test result.

Abstract: The present disclosure is drawn to methods of preparing a saliva sample for loop-mediated isothermal amplification (LAMP) detection of a pathogen target. In some embodiments, such methods can include providing an amount of saliva from a test subject, and diluting the saliva in water to a degree that reduces a buffering capacity of the saliva while maintaining a sufficient concentration to allow for detection of the pathogen target.

Abstract: The present disclosure is drawn to compositions and methods for loop-mediated isothermal amplification (LAMP) analysis utilizing a pH-dependent output signal. The composition can comprise a pH sensitive dye, and a plurality of non-interfering LAMP reagents. The method can comprise providing an assembly of a solid phase medium and a composition, depositing a biological sample onto the solid phase medium, and heating the assembly to an isothermal temperature sufficient to facilitate a LAMP reaction.