Abstract: The present invention discloses a dosage optimization method of a chemical conditioner for deeply dewatering the sludge in a municipal wastewater treatment plant, comprises the following steps: (1) optimizing a dosage of a chemical conditioner added into a specific sludge to obtain an optimal dosage proportion of the chemical conditioner corresponding to unit mass of organic matter; (2) calculating an optimal dosage of the chemical conditioner applicable for other sludge to be treated; and (3) adding the chemical conditioner into the sludge to be treated for sludge conditioning treatment, and then performing mechanical dewatering treatment, so as to obtain a deeply dewatered sludge with a moisture content of less than 60%. By improving the reference basis as the key chemical conditioner dosage, the method can effectively solve the problem that types and moisture content range of sludge to which the chemical conditioner dosage control method is applicable are narrow.