Patents by Inventor Jiankai Liu

Jiankai Liu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190240314
    Abstract: The present invention provides a method for purifying an EV71 virus-like particle and a method for preparing a vaccine thereof. The virus-like particle is obtained by performing high density fermentation cultivation on recombinantly engineered bacteria; inducing expression of the EV71 virus-like particle protein expression using methanol; collecting the bacteria by centrifugation and performing high-pressure homogenization for disruption; performing precipitation on the supernatant with ammonium sulfate; and purifying by redissolution, ultrafiltration, ion exchange chromatography, molecular sieve chromatography, hydroxyapatite chromatography, etc.
    Type: Application
    Filed: December 28, 2016
    Publication date: August 8, 2019
    Applicant: BEIJING MINHAI BIOTECHNOLOGY CO., LTD.
    Inventors: Guoshun LI, Meirong GU, Jiankai LIU, Lin GUO, Lei CHEN, Gaimei ZHANG, Yingzhi XU, Jin LI, Haifeng XIAO
  • Patent number: 9782471
    Abstract: The present invention provides EV71 virus-like particles and a preparation method and application thereof. The method comprises: connecting a P1 protein gene and a 3CD protease gene of an EV71 virus with a PMV plasmid to construct a PMV-P1-3CD recombinant expression plasmid; then transforming a Hansenula polymorpha AU-0501 expression strain with the PMV-P1-3CD recombinant expression plasmid to obtain an AU-PMV-P1-3CD recombinant expression strain; fermenting and culturing the recombinant expression strain, and inducing the recombinant expression strain to express the EV71 virus-like particle protein with methanol; centrifuging and collecting mycelia for homogeneous breakage at a high pressure; and purifying the supernatant through ion-exchange chromatography, hydrophobic chromatography, and molecular sieve chromatography, so as to obtain EV71 virus-like particles.
    Type: Grant
    Filed: April 25, 2014
    Date of Patent: October 10, 2017
    Assignee: BEIJING MINHAI BIOTECHNOLOGY CO., LTD.
    Inventors: Meirong Gu, Wenjin Wei, Jiankai Liu, Linlin Song, Shanshan Xu, Guoshun Li, Lin Guo
  • Publication number: 20160166675
    Abstract: The present invention provides EV71 virus-like particles and a preparation method and application thereof. The method comprises: connecting a P1 protein gene and a 3CD protease gene of an EV71 virus with a PMV plasmid to construct a PMV-P1-3CD recombinant expression plasmid; then transforming a Hansenula polymorpha AU-0501 expression strain with the PMV-P1-3CD recombinant expression plasmid to obtain an AU-PMV-P1-3CD recombinant expression strain; fermenting and culturing the recombinant expression strain, and inducing the recombinant expression strain to express the EV71 virus-like particle protein with methanol; centrifuging and collecting mycelia for homogeneous breakage at a high pressure; and purifying the supernatant through ion-exchange chromatography, hydrophobic chromatography, and molecular sieve chromatography, so as to obtain EV71 virus-like particles.
    Type: Application
    Filed: April 25, 2014
    Publication date: June 16, 2016
    Inventors: Meirong GU, Wenjin WEI, Jiankai LIU, Linlin SONG, Shanshan XU, Guoshun LI, Lin GUO
  • Patent number: 7648726
    Abstract: A method including forming a first coating layer derived from an alkoxide on a substrate having a dimension suitable for an implant and forming a second coating layer on the first coating layer that promotes osseointegration. An apparatus comprising a substrate having a dimension suitable as a medical or dental implant and a coating on a surface of a first coating layer derived from an alkoxide and a second coating layer that promotes osseointegration.
    Type: Grant
    Filed: March 6, 2008
    Date of Patent: January 19, 2010
    Assignee: Chemat Technology, Inc.
    Inventors: Jiankai Liu, Yuhong Huang, Ichiro Nishimura
  • Publication number: 20080152785
    Abstract: A method including forming a first coating layer derived from an alkoxide on a substrate having a dimension suitable for an implant and forming a second coating layer on the first coating layer that promotes osseointegration. An apparatus comprising a substrate having a dimension suitable as a medical or dental implant and a coating on a surface of a first coating layer derived from an alkoxide and a second coating layer that promotes osseointegration.
    Type: Application
    Filed: March 6, 2008
    Publication date: June 26, 2008
    Inventors: Jiankai Liu, Yuhong Huang, Ichiro Nishimura
  • Patent number: 7341756
    Abstract: A method including forming a first coating layer derived from an alkoxide on a substrate having a dimension suitable for an implant and forming a second coating layer on the first coating layer that promotes osseointegration. An apparatus comprising a substrate having a dimension suitable as a medical or dental implant and a coating on a surface of a first coating layer derived from an alkoxide and a second coating layer that promotes osseointegration.
    Type: Grant
    Filed: February 22, 2006
    Date of Patent: March 11, 2008
    Assignee: Chemat Technology, Inc.
    Inventors: Jiankai Liu, Yuhong Huang, Ichiro Nishimura
  • Publication number: 20060141002
    Abstract: A method including forming a first coating layer derived from an alkoxide on a substrate having a dimension suitable for an implant and forming a second coating layer on the first coating layer that promotes osseointegration. An apparatus comprising a substrate having a dimension suitable as a medical or dental implant and a coating on a surface of a first coating layer derived from an alkoxide and a second coating layer that promotes osseointegration.
    Type: Application
    Filed: February 22, 2006
    Publication date: June 29, 2006
    Inventors: Jiankai Liu, Yuhong Huang, Ichiro Nishimura
  • Patent number: 7067169
    Abstract: A method including forming a first coating layer derived from an alkoxide on a substrate having a dimension suitable for an implant and forming a second coating layer on the first coating layer that promotes osseointegration. An apparatus comprising a substrate having a dimension suitable as a medical or dental implant and a coating on a surface of a first coating layer derived from an alkoxide and a second coating layer that promotes osseointegration.
    Type: Grant
    Filed: June 4, 2003
    Date of Patent: June 27, 2006
    Assignee: Chemat Technology Inc.
    Inventors: Jiankai Liu, Yuhong Huang, Ichiro Nishimura
  • Publication number: 20040249472
    Abstract: A method including forming a first coating layer derived from an alkoxide on a substrate having a dimension suitable for an implant and forming a second coating layer on the first coating layer that promotes osseointegration. An apparatus comprising a substrate having a dimension suitable as a medical or dental implant and a coating on a surface of a first coating layer derived from an alkoxide and a second coating layer that promotes osseointegration.
    Type: Application
    Filed: June 4, 2003
    Publication date: December 9, 2004
    Inventors: Jiankai Liu, Yuhong Huang, Ichiro Nishimura