Abstract: A method and system for label-free detection of pathogenic and antibiotic resistant bacteria is disclosed. The method includes fabricating a G-FET/peptide device having a synthesized peptide probe capable of recognizing and binding to a bacterial target; performing electric-field assisted binding of at least one bacterial cell of the bacterial target to the G-FET/peptide device; and electrically detecting the binding of the at least one bacterial cell to the G-FET/peptide device.

Abstract: An ultrasonic stepping motor device for generating ultra-fine single droplet includes a valve housing, a valve core, and a spring. The valve core and the spring are installed inside the valve housing, and the valve core abuts against a liquid inlet of the valve housing under an elastic force of the spring. The valve housing is connected with a injector through a catheter, and the catheter is driven for linear operation by the inverse piezoelectric effect of piezoelectric ceramics through an ultrasonic linear motor. A right end of the catheter is equipped with a rubber piston, and the rubber piston is arranged in a cavity of the injector and matched with the injector. Since the piezoelectric vibrator driven catheter can produce nano feed length, and the volume of droplets overflowed each time can reach micron level, which can meet the requirements of producing micro droplets.

Abstract: An ultrasonic stepping motor device for generating ultra-fine single droplet includes a valve housing, a valve core, and a spring. The valve core and the spring are installed inside the valve housing, and the valve core abuts against a liquid inlet of the valve housing under an elastic force of the spring. The valve housing is connected with a injector through a catheter, and the catheter is driven for linear operation by the inverse piezoelectric effect of piezoelectric ceramics through an ultrasonic linear motor. A right end of the catheter is equipped with a rubber piston, and the rubber piston is arranged in a cavity of the injector and matched with the injector. Since the piezoelectric vibrator driven catheter can produce nano feed length, and the volume of droplets overflowed each time can reach micron level, which can meet the requirements of producing micro droplets.

Abstract: Provided is a chemically modified phage display platform and method of use thereof. More specifically, the present disclosure provides a chemically modified phage display library that incorporates 2-acetylphenylboronic acid (APBA) moieties to elicit dynamic covalent binding to the bacterial cell surface. The APBA-modified phage display libraries described herein are applicable to a wide array of bacterial strains and/or mammalian cells, paving the way to facile diagnosis and development of strain-specific antibiotics, and/or peptide-antibiotic conjugates for effective and targeted treatment. Also provided are therapeutic peptides, and pharmaceutical compositions thereof, that are identified by screening the phage display library of the present disclosure, and method of use of such therapeutic peptides for effective and targeted treatment.

Abstract: An air-assisted electrostatic ultrasonic atomization nozzle includes an intake sleeve, a Laval tube, a resonant body and a jet element body. The left end of the intake sleeve is equipped with the air intake, and the right end of the air inlet sleeve is connected with the left end of the Laval tube. The right end of the Laval tube is connected with the left end of the resonant body. The right end of the resonant body is connected with the left end of the jet element body. The sealing surface of the resonant tube is arranged between the resonant body and the jet element body. The sealing surface of the resonant tube obstructs the gas-liquid in the axial direction of the resonant body and the jet element body. The resonant body has a resonant chamber, and the sidewall of the resonant body is equipped with a V-shaped resonant tube.

Abstract: A step cavity type low frequency ultrasonic atomization nozzle with a swirlable vortex impeller, has an air intake casing, an inlet casing, a Laval core, a fixed cap, an adjustable pedestal, and taper rectifying sleeve, swirlable vortex impeller, stepped resonance tube, regulative plunger, positioning lead, second pedestal. The regulative plunger is located in the second stepped hole of the stepped resonance tube, and its axial position is adjustable; the swirlable vortex impeller is fixed on the taper resonance tube through the bearing, and the outer cone surface is matched with the inner cone surface of the taper rectifying sleeve. The resonant cavity is improved so that the two-phase fluid in the cavity can generate higher frequency and greater fluctuation of the pressure fluctuation amplitude, optimize the initial atomization performance of the nozzle, and optimize the nozzle outlet, and increase the swirlable vortex impeller.

Abstract: An air-assisted electrostatic ultrasonic atomization nozzle includes an intake sleeve, a Laval tube, a resonant body and a jet element body. The left end of the intake sleeve is equipped with the air intake, and the right end of the air inlet sleeve is connected with the left end of the Laval tube. The right end of the Laval tube is connected with the left end of the resonant body. The right end of the resonant body is connected with the left end of the jet element body. The sealing surface of the resonant tube is arranged between the resonant body and the jet element body. The sealing surface of the resonant tube obstructs the gas-liquid in the axial direction of the resonant body and the jet element body. The resonant body has a resonant chamber, and the sidewall of the resonant body is equipped with a V-shaped resonant tube.

Abstract: A low-frequency ultrasonic atomizing device includes a piezoelectric vibrator, a horn, a secondary atomizing chamber, a gas-liquid valve end cover, a Laval-type valve core, a stepped valve core, and a gas-liquid valve body. The piezoelectric vibrator is glued onto the horn, and the gas-liquid valve end cap is connected to the gas-liquid valve body by a thread, while both the stepped valve core and the Laval-type valve core are installed within a cylindrical cavity of the valve body, an end of the Laval-type valve core being sleeved at an end of the stepped valve core. The horn and the secondary atomizing chamber, the secondary atomizing chamber and the gas-liquid valve end cover are connected by a double-head stud and a nut. The device achieves multi-stage atomization of droplets, which increases the atomization quantity of a spray device, the droplets being small, and also achieves long distance spraying.

Abstract: Provided is a chemically modified phage display platform and method of use thereof. More specifically, the present disclosure provides a chemically modified phage display library that incorporates 2-acetylphenylboronic acid (APBA) moieties to elicit dynamic covalent binding to the bacterial cell surface. The APBA-modified phage display libraries described herein are applicable to a wide array of bacterial strains and/or mammalian cells, paving the way to facile diagnosis and development of strain-specific antibiotics, and/or peptide-antibiotic conjugates for effective and targeted treatment. Also provided are therapeutic peptides, and pharmaceutical compositions thereof, that are identified by screening the phage display library of the present disclosure, and method of use of such therapeutic peptides for effective and targeted treatment.

Abstract: A method and system for label-free detection of pathogenic and antibiotic resistant bacteria is disclosed. The method includes fabricating a G-FET/peptide device having a synthesized peptide probe capable of recognizing and binding to a bacterial target; performing electric-field assisted binding of at least one bacterial cell of the bacterial target to the G-FET/peptide device; and electrically detecting the binding of the at least one bacterial cell to the G-FET/peptide device.

Abstract: A pesticide droplet leaf transmembrane absorption observation apparatus: an outer anti-mist glass cover (13) is shrouded over a lower base plate (14) to form an outer anti-mist chamber, used for accommodating a whole plant, a support frame (12) being arranged inside an outer atomising chamber, an inner anti-mist glass cover (9) being shrouded over an upper top plate of the support frame (12) to form an observation chamber, an outer atomising nozzle (6) and an inner atomising nozzle (8) respectively being inserted into the outer anti-mist chamber and the observation chamber, a temperature controller (2) respectively being connected to the outer atomising nozzle (6) and the inner atomising nozzle (8); a temperature sensor (7) is arranged inside the observation chamber and is connected to a data collection computer (1), a leaf pressing mechanism (10) being arranged inside the observation chamber and being used for pressing the leaves of the plant; a first digital camera (3) and a first microscope (4) and a secon

Abstract: A pesticide droplet leaf transmembrane absorption observation apparatus: an outer anti-mist glass cover (13) is shrouded over a lower base plate (14) to form an outer anti-mist chamber, used for accommodating a whole plant, a support frame (12) being arranged inside an outer atomising chamber, an inner anti-mist glass cover (9) being shrouded over an upper top plate of the support frame (12) to form an observation chamber, an outer atomising nozzle (6) and an inner atomising nozzle (8) respectively being inserted into the outer anti-mist chamber and the observation chamber, a temperature controller (2) respectively being connected to the outer atomising nozzle (6) and the inner atomising nozzle (8); a temperature sensor (7) is arranged inside the observation chamber and is connected to a data collection computer (1), a leaf pressing mechanism (10) being arranged inside the observation chamber and being used for pressing the leaves of the plant; a first digital camera (3) and a first microscope (4) and a secon

Abstract: A step cavity low-frequency ultrasonic atomizing nozzle includes an air inlet casing tube, a water inlet casing tube, a de Laval valve, a fixed cap, a first adjustable base, a tapered rectification sleeve, a vortex flow impeller, a stepped resonance tube, an adjustment plunger, a positioning screw, and a second base. The adjustment plunger is located within a second step hole of the stepped resonance tube, and the axial position thereof is adjustable. The vortex flow impeller is fixed on the stepped resonance tube via a bearing, and an outer tapered surface thereof attaches to an inner tapered surface of the tapered rectification sleeve.

Abstract: A low-frequency ultrasonic atomizing device includes a piezoelectric vibrator, a horn, a secondary atomizing chamber, a gas-liquid valve end cover, a Laval-type valve core, a stepped valve core, and a gas-liquid valve body. The piezoelectric vibrator is glued onto the horn, and the gas-liquid valve end cap is connected to the gas-liquid valve body by a thread, while both the stepped valve core and the Laval-type valve core are installed within a cylindrical cavity of the valve body, an end of the Laval-type valve core being sleeved at an end of the stepped valve core. The horn and the secondary atomizing chamber, the secondary atomizing chamber and the gas-liquid valve end cover are connected by a double-head stud and a nut. The device achieves multi-stage atomization of droplets, which increases the atomization quantity of a spray device, the droplets being small, and also achieves long distance spraying.

Abstract: The invention discloses a low-frequency electrostatic ultrasonic atomization nozzle that relates to an electrostatic atomizer in the field of agricultural engineering. The low-frequency electrostatic ultrasonic atomization nozzle comprises a transducer back cover, piezoelectric ceramics, a transducer front cover, an ultrasonic horn and a fastening screw. Furthermore, the fastening screw is set through the transducer back cover, the piezoelectric ceramics and the center round hole of the transducer front cover in sequence; a liquid inlet channel is designed in the axial center of the ultrasonic horn; an air intake channel is designed in a position that deviates from the axial center; the top of the ultrasonic horn is machined as a concave spherical surface; and a suspended ball is arranged on the concave spherical surface.

Abstract: An oblique submerged reverse deep rotary tilling device configured to be connected to a tractor. The tilling device includes a stander, a mounted frame, a gearbox and an oblique blade roller. The oblique blade roller has configured rotary blades whose tips bend to the same direction. The angle between the rotary tilling blade roller and an advancing direction of the tractor is 55˜70° while the rotary blade tips are all toward the right side of the advancing direction. Side force balance blades are installed to the left side of the oblique blade roller, rotate in a plane that is parallel to the advancing direction, and have a rotating center axis disposed at an angle to the oblique blade roller to balance the axial component force of the oblique blade roller.