Abstract: Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.

Abstract: To enable automatic calculation of a circuit element value and a waveform by a computer. In a circuit design support for calculating a circuit element value of an analog electronic circuit to be designed, by causing a computer to execute a program having a description of a repetition calculation equation of the Newton method including the Jacobi matrix, a program of the aforementioned calculation equation in which a partial differentiation as an element in the Jacobi matrix is replaced by an approximation equation is used. Furthermore, the element to be subjected to the partial differentiation in the Jacobi matrix is obtained from the waveform observed in response to the circuit configuration of the analog electronic circuit.

Abstract: Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.

Abstract: To enable automatic calculation of a circuit element value and a waveform by a computer. In a circuit design support for calculating a circuit element value of an analog electronic circuit to be designed, by causing a computer to execute a program having a description of a repetition calculation equation of the Newton method including the Jacobi matrix, a program of the aforementioned calculation equation in which a partial differentiation as an element in the Jacobi matrix is replaced by an approximation equation is used. Furthermore, the element to be subjected to the partial differentiation in the Jacobi matrix is obtained from the waveform observed in response to the circuit configuration of the analog electronic circuit.

Abstract: The present invention relates to improved cell-based assays for the in vivo assessment of phosphodiesterase (PDE) activity using cyclic nucleotide-gated channels as cyclic nucleotide sensors, and for the assessment of the effect of PDE modulating compounds.

Abstract: Disclosed are methods for manufacturing radiohalogen-labeled steroids, nucleosides, nucleotides and proteins, for example, pyrimidines and proteins. Methods according to the example embodiments specific to pyrimidines includes dissolving a halogenated pyrimidine to obtain an initial solution, adding bis(tributyltin) and triphenylphosphine palladium to produce a catalyzed solution, heating the catalyzed solution under conditions sufficient to induce a reaction that produces an intermediate solution including a stannylated pyrimidine compound, removing substantially all of the first solvent from the intermediate solution to obtain an intermediate composition, purifying the resulting solution to obtain a purified composition including a stannylated pyrimidine, reacting the stannylated pyrimidine compound with a radiohalogen in a buffered reaction mixture to produce the radiohalogenated pyrimidine, adding an antioxidant and extracting the radiohalogenated pyrimidine from the reaction mixture.

Abstract: Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.

Abstract: Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.

Abstract: The invention relates to an elastic bandage for medical use. A base part of the bandage is made of pure cotton cloth or other fabric with a cloth cover on a transverse side in which a drainage aperture is defined. On opposite transverse sides of the base part there are respectively provided two outlying parts of the bandage with a drainage aperture in each of them. On opposite transverse sides of the base part there are respectively two safety bands and two suspenders on a longitudinal side of base part. The safety bands and the outlying parts are fastened together with fasteners.