Patents by Inventor Jianming Xie

Jianming Xie has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9102932
    Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.
    Type: Grant
    Filed: June 8, 2012
    Date of Patent: August 11, 2015
    Assignee: The Scripps Research Institute
    Inventors: Peter G. Schultz, Lital Alfonta, Johnathan R. Chittuluru, Alexander Deiters, Dan Groff, Daniel Summerer, Meng-Lin Tsao, Jiangyun Wang, Ning Wu, Jianming Xie, Huaqiang Zeng, Mohammad Seyedsayamdost, James Turner
  • Publication number: 20140120557
    Abstract: Methods for labeling and/or detecting a T cell according to specificity of an antigen T cell receptor (TCR) are provided. Also provided are monomeric MHC-peptide complexes and kits for crosslinking to a T cell according to specificity of an antigen T cell receptor (TCR). The methods, monomeric MHC-peptide complexes and kits find use in a variety of applications related to the detection and purification of antigen-specific T cells, such as those T cells involved in tumors, infectious diseases and autoimmune diseases.
    Type: Application
    Filed: October 2, 2013
    Publication date: May 1, 2014
    Inventors: Jianming Xie, Mark M. Davis
  • Patent number: 8673591
    Abstract: This invention provides reductase proteins that comprise NH2Y unnatural amino acid residues, systems of orthogonal elements for incorporating NH2Y into reductases and methods of using NH2Y amino acid residues in reductases as molecular probes for probing reductases function, structure and activity.
    Type: Grant
    Filed: October 23, 2008
    Date of Patent: March 18, 2014
    Assignees: The Scripps Research Institute, Massachusetts Institute of Technology
    Inventors: Mohammad R. Seyedsayamdost, Jianming Xie, Clement Tsz Chan, Lital Alfonta, Peter G. Schultz, Joanne Stubbe
  • Publication number: 20130244245
    Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.
    Type: Application
    Filed: June 8, 2012
    Publication date: September 19, 2013
    Applicant: The Scripps Research Institute
    Inventors: Peter G. Schultz, Lital Alfonta, Johnathan R. Chittuluru, Alexander Deiters, Dan Groff, Daniel Summerer, Meng-Lin Tsao, Jiangyun Wang, Ning Wu, Jianming Xie, Huaqiang Zeng, Mohammad Seyedsayamdost, James Turner
  • Publication number: 20120329162
    Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.
    Type: Application
    Filed: April 30, 2012
    Publication date: December 27, 2012
    Applicant: The Scripps Research Institute
    Inventors: Jiangyun Wang, Jianming Xie, Peter G. Schultz
  • Patent number: 8216804
    Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.
    Type: Grant
    Filed: October 27, 2005
    Date of Patent: July 10, 2012
    Assignee: The Scripps Research Institute
    Inventors: Peter G. Schultz, Jianming Xie, Huaqiang Zeng
  • Patent number: 8192952
    Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.
    Type: Grant
    Filed: May 21, 2007
    Date of Patent: June 5, 2012
    Assignee: The Scripps Research Institute
    Inventors: Jiangyun Wang, Jianming Xie, Peter G. Schultz
  • Publication number: 20110262949
    Abstract: This invention provides reductase proteins that comprise NH2Y unnatural amino acid residues, systems of orthogonal elements for incorporating NH2Y into reductases and methods of using NH2Y amino acid residues in reductases as molecular probes for probing reductases function, structure and activity.
    Type: Application
    Filed: October 23, 2008
    Publication date: October 27, 2011
    Inventors: Mohammad R. Seyedsayamdost, Jianming Xie, Clement Tsz Chan, Lital Alfonta, Peter G. Schultz, Joanne Stubbe
  • Patent number: 7807411
    Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl) ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.
    Type: Grant
    Filed: May 21, 2007
    Date of Patent: October 5, 2010
    Assignee: The Scripps Research Institute
    Inventors: Jiangyun Wang, Jianming Xie, Peter G. Schultz
  • Publication number: 20090298119
    Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.
    Type: Application
    Filed: May 21, 2007
    Publication date: December 3, 2009
    Inventors: Jiangyun Wang, Jianming Xie, Peter G. Schultz
  • Publication number: 20090208994
    Abstract: This invention provides reductase proteins that comprise NH2Y unnatural amino acid residues, systems of orthogonal elements for incorporating NH2Y into reductases and methods of using NH2Y amino acid residues in reductases as molecular probes for probing reductases function, structure and activity.
    Type: Application
    Filed: October 23, 2008
    Publication date: August 20, 2009
    Applicants: THE SCRIPPS RESEARCH INSTITUTE, MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Mohammad R. Seyedsayamdost, Jianming Xie, Tsz Yan Clement Chan, Lital Alfonta, Peter G. Schultz, Joanne Stubbe
  • Publication number: 20090181858
    Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal leucyl-tRNAs, orthogonal leucyl-aminoacyl-tRNA synthetases, and orthogonal pairs of leucyl-tRNAs/synthetases, which incorporate photoregulated amino acids, OMe-L-tyrosine, ?-aminocaprylic acid, or o-nitrobenzyl cysteine into proteins are proteins are provided in response to an amber selector codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with a photoregulated amino acid, Ome-L-tyrosine, ?-aminocaprylic acid, or o-nitrobenzyl cysteine using these orthogonal pairs.
    Type: Application
    Filed: September 21, 2005
    Publication date: July 16, 2009
    Applicant: The Scripps Research Institute
    Inventors: Alexander Deiters, Ning Wu, Peter G. Schultz, David King, T. Ashton Cropp, Mohua Bose, Dan Groff, Jianming Xie, Eric Brustad
  • Publication number: 20080233611
    Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.
    Type: Application
    Filed: October 27, 2005
    Publication date: September 25, 2008
    Applicant: THE SCRIPPS RESEARCH INSTITUTE
    Inventors: Peter G. Schultz, Lital Alfonta, Jonathan R. Chittuluru, Alexander Deiters, Dan Groff, Daniel Summerer, Meng-Lin Tsao, Jiangyun Wang, Ning Wu, Jianming Xie, Huaqiang Zeng
  • Patent number: 7399619
    Abstract: Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.
    Type: Grant
    Filed: May 24, 2005
    Date of Patent: July 15, 2008
    Assignee: The Scripps Research Institute
    Inventors: Jianming Xie, Lei Wang, Ning Wu, Peter G. Schultz
  • Publication number: 20080044886
    Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl) ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.
    Type: Application
    Filed: May 21, 2007
    Publication date: February 21, 2008
    Applicant: The Scripps Research Institute
    Inventors: Jiangyun Wang, Jianming Xie, Peter Schultz
  • Publication number: 20060134746
    Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal leucyl-tRNAs, orthogonal leucyl-aminoacyl-tRNA synthetases, and orthogonal pairs of leucyl-tRNAs/synthetases, which incorporate photoregulated amino acids, OMe-L-tyrosine, ?-aminocaprylic acid, or o-nitrobenzyl cysteine into proteins are provided in response to an amber selector codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with a photoregulated amino acid, OMe-L-tyrosine, ?-aminocaprylic acid, or o-nitrobenzyl cysteine using these orthogonal pairs.
    Type: Application
    Filed: September 21, 2005
    Publication date: June 22, 2006
    Applicant: The Scripps Research Institute
    Inventors: Alexander Deiters, Ning Wu, Peter Schultz, David King, T. Cropp, Mohua Bose, Dan Groff, Jianming Xie, Eric Brustad
  • Publication number: 20050272121
    Abstract: Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.
    Type: Application
    Filed: May 24, 2005
    Publication date: December 8, 2005
    Applicants: The Scripps Research Institute, IRM, LLC
    Inventors: Jianming Xie, Lei Wang, Ning Wu, Peter Schultz, Glen Spraggon
  • Patent number: 5767103
    Abstract: The present invention describes a method of sequestering nitric oxide from the bloodstream, endothelium or tissues of mammals by administering a cobalamin to such mammals. A method of treating diseases characterized by elevated nitric oxide levels in the bloodstream, endothelium or tissues is further provided. In particular, a method of treating sepsis by administering a therapeutic dose of hydroxocobalamin is described by the present invention. A method of alleviating systemic hypotension in a septic patient is further described by this invention. A pharmaceutical composition comprising a cobalamin in a concentration ranging from 0.5 to 50 mg composition/Kg body weight for mammals and is also provided.
    Type: Grant
    Filed: April 19, 1995
    Date of Patent: June 16, 1998
    Assignee: Research Corporation Tech., Inc.
    Inventors: Stanley S. Greenberg, Jianming Xie, Mark J. S. Miller, Judith Zatarian-Kuebel