Abstract: The invention discloses an exopolysaccharide from Rhodopseudomonas palustris and a method for preparing and use thereof, and the method for preparing comprises the steps of: 1) keeping a seed solution from Rhodopseudomonas palustris GJ-22 in a fermentation medium for fermentation culture to obtain a fermentation broth; 2) centrifuging the fermentation broth to take the supernatant, which is treated by alcohol precipitation after filtration, and then collecting the pellet from alcohol precipitation by centrifugation to obtain crude polysaccharide; 3) removing proteins from the rude polysaccharide using protease enzymolysis method and Sevag method, followed by dialysis treatment with distilled water to remove small molecules and organic solvent to obtain a polysaccharide sample; 4) purifying the polysaccharide sample through an anion exchange column and a molecular exclusion chromatography column obtain the exopolysaccharide from Rhodopseudomonas palustris.

Abstract: The invention discloses an exopolysaccharide from Rhodopseudomonas palustris and a method for preparing and use thereof, and the method for preparing comprises the steps of: 1) keeping a seed solution from Rhodopseudomonas palustris GJ-22 in a fermentation medium for fermentation culture to obtain a fermentation broth; 2) centrifuging the fermentation broth to take the supernatant, which is treated by alcohol precipitation after filtration, and then collecting the pellet from alcohol precipitation by centrifugation to obtain crude polysaccharide; 3) removing proteins from the rude polysaccharide using protease enzymolysis method and Sevag method, followed by dialysis treatment with distilled water to remove small molecules and organic solvent to obtain a polysaccharide sample; 4) purifying the polysaccharide sample through an anion exchange column and a molecular exclusion chromatography column obtain the exopolysaccharide from Rhodopseudomonas palustris.

Abstract: A payment method based on a member feature code includes: acquiring a member feature code of a store user collected by a store aiming at an order to be paid (S101); identifying a member ID corresponding to the member feature code (S102); generating a discounted order corresponding to the order to be paid according to membership benefits of the member (S103); and paying for the discounted order based on a payment account corresponding to the member ID (S104). During the payment between the store user and the store, the present payment method based on the member feature code helps the store to cultivate the familiarity of the store user with the membership benefits, and meanwhile, enables the payment between the store user and the store to be quicker and more convenient, optimizing the payment experience between the store user and the store.