Abstract: The invention provides compositions of matter, methods and treatment means for improving cosmetic appearance of skin and restoring aged or damaged skin to a healthy appearance. One embodiment, the invention teaches administration of autologous adipose derived mesenchymal stem cells that have been cultured under “activating conditions”. In one specific embodiment activation is performed prior to administration of cells into patient's skin. In another embodiment adipose derived cells are administered systemically, with localization of stem cells to skin by administration of a localizing agent, said localizing agent comprising either a peptide; a protein; or a photoceutical.

Abstract: This patent discloses new data supporting superior collagen induction by cosmetically useful preparations derived from adipose stem cells that have been manipulated for superior growth factor and anti-aging properties. In one embodiment cellular mixtures derived from adipose tissue are composed, induced to produce regenerative factors, with said regenerative factors harvested and compounded into cosmetic preparations. In one embodiment, the invention provides for manufacture of adipose derived regenerative factor (ARDF), which may be utilized as a cosmetic and skin rejuvenating agent.