Abstract: A method of forming and preserving a bioremodelable, biopolymer scaffold material is carried out by subjecting animal tissue, particularly fetal or neo-natal tissue, to chemical and mechanical processing. The process may include, but is not limited to, harvesting the tissue, optionally extracting growth and differentiation factors from the tissue, inactivating infective agents of the tissue such as by treating with KOH or NaOH, mechanically expressing undesirable components from the tissue, delipidizing the tissue with organic solvents, mechanically expressing components released by the solvet, washing the tissue, optionally drying the tissue, and optionally cross-linking the tissue, not necessarily in the order described. The resulting product, named EB Matrix (EBM), is characterized by its microbial, fungal, viral and prion inactivated state. EBM is strong, bioremodelable, drapable and does not undergo calcification.

Abstract: The applications of this invention, the use of stem cells derived from the dermis, include but are not limited to cell differentiation, histiogenesis, organogenesis, cell therapy, tissue engineering, and tissue and organ regeneration.

Abstract: A method of forming and preserving a bioremodelable, biopolymer scaffold material by subjecting animal tissue to chemical and mechanical processing. In addition to skin tissue, another source of EBM is a blood vessel. EBM may be used for hernia repair, colon, rectal, vaginal and or urethral prolapse treatment; pelvic floor reconstruction; muscle flap reinforcement; lung tissue support; rotator cuff repair or replacement; periosteum replacement; dura repair; pericardial membrane repair; soft tissue augmentation; intervertebral disk repair; and periodontal repair. EBM may also be used as a urethral sling, laminectomy barrier or spinal fusion device.

Abstract: Methods of forming and using signaling complexes (“Signalplexes”) expressed in specific tissues and organs during particular stages of development to predicably guide cell division, cell migration, differentiation of cells and/or formation of tissue or organ primordia in vitro.

Abstract: Methods and compositions for repairing tissue. Certain embodiments of the invention involve transdifferentiation of cells in a manner not heretofore provided for. One embodiment of the invention features methods for producing stem cells. These methods can involve exposing cells (e.g., human fibroblasts) to a processed or activated egg extract (e.g., activated egg extract); and culturing the cells for a period of time to become stem cells. A cell culture can be performed in two or three dimensions, so that organ tissue or whole organs may be produced, e.g., for transplantation. Another embodiment of the invention features methods for promoting wound healing by using signaling complexes.

Abstract: This invention includes methods and compositions for generating signaling complexes (“Signal-plexes”) and the use of Signal-plexes for inducing cell divisions, differentiation and transdifferentiation of cells into specific cell, tissue or organ types which resemble the cells, tissues or organs from which the Signal-plexes were derived.

Abstract: The applications of this invention, the use of stem cells derived from the dermis, include but are not limited to cell differentiation, histiogenesis, organogenesis, cell therapy, tissue engineering, and tissue and organ regeneration.