Abstract: The invention relates to a method for the production of adenosine 3?,5?-monophosphate (cAMP) by a permeable microbial strain which uses polyols for protecting activities of enzymes and a cAMP precursor and phosphate as substrates. Glucose is used as an energy provider and metal ions and an organic solvent such as acetone are used in the medium.

Abstract: The present invention provides Clostridium acetobutylicum and an application thereof. A preservation number of the Clostridium acetobutylicum provided in the invention is CGMCC No. 5234. The Clostridium acetobutylicum provided in the present invention can be used for cogeneration of acetone, butanol, ethanol, and 3-hydroxy butanone through fermentation, so as to improve the economic benefit of butanol fermentation. NAD+ coupling and regeneration can be implemented by adding metabolism or growth regulating substances, so as to improve the product yield, and at the same time, the yield of cogeneration products can be flexibly adjusted, so as to cater for the market demand.

Abstract: The present invention provides a method for desorbing and regenerating a butanol-adsorbing hydrophobic macroporous polymer adsorbent, comprising: successively eluting the hydrophobic macroporous polymer adsorbent with butanol adsorbed therein using a water soluble low-boiling-point polar solvent and water. The method provided in the present invention has a simple process, a short separation time, easy, fast and complete desorption and regeneration, low equipment investment and pollution, and reduced energy consumption, and therefore production is easy on a large scale.

Abstract: The present invention provides Clostridium acetobutylicum and an application thereof. A preservation number of the Clostridium acetobutylicum provided in the invention is CGMCC No. 5234. The Clostridium acetobutylicum provided in the present invention can be used for cogeneration of acetone, butanol, ethanol, and 3-hydroxy butanone through fermentation, so as to improve the economic benefit of butanol fermentation. NAD+ coupling and regeneration can be implemented by adding metabolism or growth regulating substances, so as to improve the product yield, and at the same time, the yield of cogeneration products can be flexibly adjusted, so as to cater for the market demand.

Abstract: Disclosed is a method for separating butanol. The method uses hydrophobic macroporous polymer adsorbent to separate butanol in a mixed solution, and the process comprises the following steps: 1) using macroporous polymer adsorbent to adsorb butanol in a mixed solution; 2) desorbing butanol from macroporous polymer adsorbent. The method is simple; the separation time is short; the efficiency of butanol recovery is high; and the separating cost is low.

Abstract: The present invention provides a method for desorbing and regenerating a butanol-adsorbing hydrophobic macroporous polymer adsorbent, comprising: successively eluting the hydrophobic macroporous polymer adsorbent with butanol adsorbed therein using a water soluble low-boiling-point polar solvent and water. After a butanol-containing solution, such as a butanol fermentation liquor, has been adsorbed by a hydrophobic macroporous polymer adsorbent, the butanol adsorbed in the adsorbent can be thoroughly dissolved and removed by the desorption and regeneration method of the present invention; furthermore, the adsorbent can be directly regenerated to recover its adsorption capability on a fixed bed adsorbing the butanol fermentation liquor, wherein the next stage of adsorption process can be directly entered without taking the adsorbent out from the fixed bed; therefore it saves a great deal of time and improves the regeneration efficiency.

Abstract: Disclosed is a method for separating butanol. The method uses hydrophobic macroporous polymer adsorbent to separate butanol in a mixed solution, and the process comprises the following steps: 1) using macroporous polymer adsorbent to adsorb butanol in a mixed solution; 2) desorbing butanol from macroporous polymer adsorbent. The method is simple; the separation time is short; the efficiency of butanol recovery is high; and the separating cost is low.

Abstract: Provided is a crystallization process of cyclic adenosine 3?,5?-monophosphate, which comprises the following steps: 1) reacting an aqueous solution of cyclic adenosine 3?,5?-monophosphate with a base to obtain a salt of cyclic adenosine 3?,5?-monophosphate; 2) reacting the cyclic adenosine 3?,5?-monophosphate salt solution obtained in step 1) with an acid to obtain cyclic adenosine 3?,5?-monophosphate; 3) keeping cyclic adenosine 3?,5?-monophosphate obtained in step 2) at 0-15° C.

Abstract: Disclosed is a method for the production of cyclic adenosine 3?,5?-monophosphate (cAMP) by permeable microbial strain, which uses polyols for protecting activities of enzymes, uses cAMP precursor and phosphate radical ions as substrates, uses glucose as energy provider, and uses metal ions in the mendium. The method is simple, economical and has high yield.