Abstract: A key-value store and a file system are integrated together to provide improved operations. The key-value store can include a log engine, a hash engine, a sorting engine, and a garbage collection manager. The features of the key-value store can be configured to reduce the number of I/O operations involving the file system, thereby improving read efficiency, reducing write latency, and reducing write amplification issues inherent in the combined key-value store and file system.

Abstract: Decoupled computing systems include layers of same-type computing resources, and include a dispatch layer to assign tasks from one layer to another, such as input and output (I/O) flows. The I/O flows can be assigned to particular computing resources of a layer based on a weighted moving average of performance data for the layer. When traffic is high, the assignment can include random assignment to some or all of the computing resources in the layer. The I/O flows can be split between read-intensive and write-intensive flows, with more read-intensive flows being assigned based on a pick ratio.

Abstract: A file system particular for use with key-value stores is provided. The file system can operate in a user space instead of a kernel space. The file system can be an append-only file system. The file system can support use of solid state drives (SSDs) for storage, including zoned SSDs. The file system can include a file manager, a metadata manager, a task scheduler, a space allocator, and a collaborator for collaborating with a key-value store.

Abstract: A gRNA molecule includes a first domain complementary to at least a portion of TCOF1 gene, and a second domain for binding with Cas9 protein, and a kit includes the gRNA molecule. A method of treating a subject suffering from a cancer associated with an elevated expression of TCOF1 gene includes the step of administering an effective amount of a first recombinant vector comprising the gRNA molecule of the invention to the subject. A method of inhibiting the expression of TCOF1 gene in cancer cells associated with an elevated expression of TCOF1 gene includes contacting the cancer cells with a first recombinant vector, wherein the first recombinant vector comprises a gRNA molecule comprising a first domain complementary to at least a portion of TCOF1 gene and a second domain binding with Cas9 protein.

Abstract: A gRNA molecule includes a first domain complementary to at least a portion of TCOF1 gene, and a second domain for binding with Cas9 protein, and a kit includes the gRNA molecule. A method of treating a subject suffering from a cancer associated with an elevated expression of TCOF1 gene includes the step of administering an effective amount of a first recombinant vector comprising the gRNA molecule of the invention to the subject. A method of inhibiting the expression of TCOF1 gene in cancer cells associated with an elevated expression of TCOF1 gene includes contacting the cancer cells with a first recombinant vector, wherein the first recombinant vector comprises a gRNA molecule comprising a first domain complementary to at least a portion of TCOF1 gene and a second domain binding with Cas9 protein.