Abstract: The present invention relates to a medium for porcine pluripotent stem cells and use thereof, specifically provides a medium comprising: a first component that is IWR-1-endo; a second component that is selected from WH-4-023 or A419259; and a third component that is selected from fibroblast growth factors. The medium is easy to support the establishment of stable porcine pluripotent stem cell lines, especially the porcine E8-10 pre-gastrulation epiblast stem cell lines (known as pgEpiSCs).

Abstract: Pig embryo-derived pluripotent stem cells, having the characteristics and pluripotency of pig pre-gastrulation metaplasia epiblast cells. The solution is capable of stable passage, and is able to withstand multiple instances of consecutive gene editing and produce cloned pigs. The pig embryonic pluripotent stem cells create a new path for biological research, animal husbandry, and regenerative biomedicine.

Abstract: Determining an active jacking force of a tunneling closely undercrossing existing station, includes: acquiring design parameters of stations, and geological parameters of a construction site; obtaining a stress solution expression of the active jacking force on the existing station to obtain a stress solution of any point in a semi-infinite space under the action of a vertical load, replacing the vertical load with a vertical load acting on the existing station, and obtain a vertical stress solution of the existing station; obtaining a relational expression between the deflection and the stress solution of the existing station; and substituting the stress solution expression of the active jacking force into the relational expression between the deflection and the stress solution of the existing station according to boundary conditions at both ends of a beam on elastic foundation to obtain the active jacking force that controls the deflection of the existing station.

Abstract: Determining an active jacking force of a tunneling closely undercrossing existing station, includes: acquiring design parameters of stations, and geological parameters of a construction site; obtaining a stress solution expression of the active jacking force on the existing station to obtain a stress solution of any point in a semi-infinite space under the action of a vertical load, replacing the vertical load with a vertical load acting on the existing station, and obtain a vertical stress solution of the existing station; obtaining a relational expression between the deflection and the stress solution of the existing station; and substituting the stress solution expression of the active jacking force into the relational expression between the deflection and the stress solution of the existing station according to boundary conditions at both ends of a beam on elastic foundation to obtain the active jacking force that controls the deflection of the existing station.

Abstract: A composite support structure, a construction system, and a method, the composite support structure includes a plurality of arc plate rings that are longitudinally arranged along a roadway. A concrete fill steel tube support is arranged on an inner side or an outer side of each arc plate ring. The arc plate ring is formed by splicing a plurality of arc plates. Each concrete fill steel tube support is formed by splicing a plurality of steel pipe sections. The arc plate rings and the concrete fill steel tube supports are capable of jointly supporting walls of the roadway. The support structure has high bearing capability, high construction efficiency of a construction system, and low labor intensity.

Abstract: A composite support structure, a construction system, and a method, the composite support structure includes a plurality of arc plate rings that are longitudinally arranged along a roadway. A concrete fill steel tube support is arranged on an inner side or an outer side of each arc plate ring. The arc plate ring is formed by splicing a plurality of arc plates. Each concrete fill steel tube support is formed by splicing a plurality of steel pipe sections. The arc plate rings and the concrete fill steel tube supports are capable of jointly supporting walls of the roadway. The support structure has high bearing capability, high construction efficiency of a construction system, and low labor intensity.

Abstract: We provide for the use of Tbx3 (GenBank Accession Number: NM_005996.3 (SEQ ID NO. 1), NP_005987.3 (SEQ ID NO. 2), NM_016569.3 (SEQ ID NO. 3), NP_057653.3 (SEQ ID NO. 4)) in a method of enhancing or inducing pluripotency in a cell such as a somatic cell. We describe a method of reprogramming a cell, the method comprising modulating the expression and/or activity of Tbx3 in the cell. The cell may become a pluripotent cell such as a stem cell. We further describe a method of causing a cell such as a somatic cell to display one or more characteristics of a pluripotent cell, the method comprising modulating the expression and/or activity of Tbx3 in the cell. The method may further comprise modulating the expression and/or activity of one or more, a combination of or all of Oct4, Sox2 and Klf4 in the cell.

Abstract: We provide for the use of Tbx3 (GenBank Accession Number: NM_005996.3 (SEQ ID NO. 1), NP_005987.3 (SEQ ID NO. 2), NM_016569.3 (SEQ ID NO. 3), NP_057653.3 (SEQ ID NO. 4)) in a method of enhancing or inducing pluripotency in a cell such as a somatic cell. We describe a method of reprogramming a cell, the method comprising modulating the expression and/or activity of Tbx3 in the cell. The cell may become a pluripotent cell such as a stem cell. We further describe a method of causing a cell such as a somatic cell to display one or more characteristics of a pluripotent cell, the method comprising modulating the expression and/or activity of Tbx3 in the cell. The method may further comprise modulating the expression and/or activity of one or more, a combination of or all of Oct4, Sox2 and Klf4 in the cell.