Abstract: The present invention discloses an analysis method and system for detecting vascular structures at arterial, capillary, and venous phases from time-series digital subtraction angiographic images. The method comprises the steps of: acquiring a time-series dataset of a subject using at least one rotating x-ray source and detector pair; administrating a contrast media to a blood vessel of the subject during the data acquisition; applying a motion artifact correction to the time-series dataset; and applying a segmentation method to the time-series dataset for identifying vascular structures with different flow patterns of the contrast media. The invention offers the potential to efficiently provide quantitative flow changes inside the clinical operation room without manual selection and motion artifact error.