Abstract: The disclosure describes a device and a method for high pressure and steaming heat-induced antigen retrieval. The device comprises a lower support mechanism, an isolation mechanism, a loading mechanism, a slide loading mechanism and a positioning mechanism, wherein the loading mechanism comprises at least two slide loading trays; the isolation mechanism comprises at least one upright post; and the at least two slide loading trays are sequentially arranged from bottom to top. The slide loading mechanism comprises at least one slice fixation box, and a slide glass and a cover glass arranged in the slice fixation box. The cover glass is placed on the upper surface of the slide glass, and the tissue to be retrieved is placed between the slide glass and the cover glass. A limiting hole is formed in the middle of the slide loading tray, and a plurality of air holes are formed in the slide loading tray.