Abstract: The present invention discloses primers, probes, a kit and a method for detecting Enterocytozoon hepatopenaei (EHP) pathogens of Litopenaeus vannamei. A PCR reaction system adopted in the method includes: a forward primer having a nucleotide sequence as shown in SEQ ID NO: 1, a reverse primer having a nucleotide sequence as shown in SEQ ID NO: 2, a forward primer having a nucleotide sequence as shown in SEQ ID NO: 4, a reverse primer having a nucleotide sequence as shown in SEQ ID NO: 5, a probe having a nucleotide sequence as shown in SEQ ID NO: 3, and a probe having a nucleotide sequence as shown in SEQ ID NO: 6. The method can perform duplex quantitative fluorescence PCR, an internal reference and a sample to be detected are put in the same reaction, thereby eliminating unstable factors of two reactions and improving the reliability of the result.

Abstract: A pixel structure, a method for forming the pixel structure, and a display screen are provided. The method includes: providing a substrate for forming an OLED device, the substrate having a first pixel area, a second pixel area, and a third pixel area; and forming a compensation layer on the substrate, the compensation layer having different thicknesses in the first pixel area, the second pixel area, and the third pixel area. In the present disclosure, the compensation layer is formed on the substrate, and the compensation layer has different thicknesses respectively in the first pixel area, the second pixel area and the third pixel area, so that the cavity of the first pixel area, the cavity of the second pixel area, and the cavity of the third pixel area can be individually controlled.

Abstract: A shadow mask used for OLED evaporation and a manufacturing method therefor, and an OLED panel manufacturing method. The shadow mask used for OLED evaporation includes: a semiconductor substrate including a front surface and a back surface opposite thereto, a recess penetrating the front surface and the back surface being provided in the semiconductor substrate; and a grid film layer provided on the front surface of the semiconductor substrate. A number of openings arranged in an array are provided in the grid film layer. Each of the openings has an upper portion and a lower portion. A width of the upper portion is greater than a width of the lower portion. The recess exposes the number of openings in the grid film layer and the grid film layer between adjacent openings.

Abstract: The present invention discloses a power transmission control method and device for a crane and the crane. The method includes: setting the maximum working displacement of a secondary element corresponding to each gear of the crane; determining the current gear state and specific gear of the crane; determining the working mode of the secondary element from the gear state, and setting the maximum allowable displacement of the secondary element as the maximum working displacement corresponding to the specific gear.

Abstract: The present disclosure provides compositions and methods for enhanced expression of exogenous genes in eukaryotic cells. Also provided are chromosomal loci, sequences for enhanced and stable expression of exogenous genes.

Abstract: Provided is a genetically modified non-human mammal that comprises an anchor DNA sequence inserted at an endogenous locus of a secretory milk protein gene, wherein the anchor DNA sequence comprises a site-specific recombinase recognition site. Also provided is a genetically modified non-human mammal that comprises a transgene inserted at an endogenous locus of a secretory milk protein gene, wherein the transgene encodes a secretory protein and is operably linked to the endogenous promoter of said secretory milk protein gene, and wherein the transgene is flanked by a pair of site-specific recombinase resulting sites. The genetically modified non-human mammals provided can be used for producing the secreted recombinant protein encoded by the transgene from the milk produced by the genetically modified non-human mammals.

Abstract: Provided is a method for knocking in a gene of interest to a cell. The genome of the cell contains a negative selectable marker, e.g., a thymidine kinase gene flanked by a pair of recombinase recognition sites (RRS), e.g., attP. The method involves introducing into the cell a targeting construct that contains a gene of interest flanked by a second pair of RRS, e.g., attB. The targeting construct also contains in the vector backbone a negative selectable marker, e.g., thymidine kinase gene. When a recombinase recognizing the RRS is expressed, the recombination events between the two pairs of RRS result in the site-specific integration of the gene of interest in the genome of the cell. Upon selection based on the negative selectable marker, the parental cells, cells with undesired integration, e.g., random integration, or the integration of the vector backbone are removed.

Abstract: The present disclosure provides a composition comprising a site-specific nuclease domain capable of cleaving a target DNA sequence; and a sequence-specific DNA binding domain capable of specifically binding to a recognition DNA sequence, wherein the site-specific nuclease domain operably links to the sequence-specific DNA binding domain.

Abstract: A power transmission control method and device for a crane and the crane includes setting the maximum working displacement of a secondary element corresponding to each gear of the crane; determining the current gear state and specific gear of the crane; determining the working mode of the secondary element from the gear state, and setting the maximum allowable displacement of the secondary element as the maximum working displacement corresponding to the specific gear. In the power transmission control method and device for the crane and the crane of the present invention, the maximum displacement of the secondary element is controlled for various gears, which can reduce power impact. A buffer mode is added to avoid influence on a vehicle due to loss of instant power generated at the moment of energy release completion and avoid vehicle jitter due to instable power resulting from the vehicle at low speed or a system suddenly disconnected.

Abstract: A method for network equipment to generate authentication information in the mobile communication system, at least includes the following process: set security key corresponding to mobile terminal in network equipment; and network equipment generates authentication information corresponding to mobile terminal based on said security key and a random number. And, a method for mobile terminal to authenticate communication network in the mobile communication network, at least includes the following process: set security key corresponding to mobile terminal in the mobile terminal; and after the authentication information from network equipment is received, the mobile terminal determines whether the network authentication succeeds based on said security key set by the mobile terminal and the authentication information.

Abstract: An authentication method and a method for transmitting authentication information, and the method for transmitting authentication information includes: an MS and a network agree in advance one or more agreed operations to be performed when the network determines that the SQNMS is an agreed value; the MS generating an authentication resynchronization token (AUTS) by using the agreed value, sending a resynchronization request containing the AUTS to the network; upon receiving the resynchronization request and determining that the SQNMS of the AUTS is the agreed value, the network performing the one or more agreed operations. The method of transmitting authentication information from the MS to the network conveniently implement the information transmitting between the MS and the network and guarantee the network security without increasing and changing the existing signaling resources and authentication parameters.

Abstract: An authentication method and a method for transmitting authentication information, and the method for transmitting authentication information includes: an MS and a network agree in advance one or more agreed operations to be performed when the network determines that the SQNMS is an agreed value; the MS generating an authentication resynchronization token (AUTS) by using the agreed value, sending a resynchronization request containing the AUTS to the network; upon receiving the resynchronization request and determining that the SQNMS of the AUTS is the agreed value, the network performing the one or more agreed operations. The method of transmitting authentication information from the MS to the network conveniently implement the information transmitting between the MS and the network and guarantee the network security without increasing and changing the existing signaling resources and authentication parameters.

Abstract: A method for network equipment to generate authentication information in the mobile communication system, at least includes the following process: set security key corresponding to mobile terminal in network equipment; and network equipment generates authentication information corresponding to mobile terminal based on said security key and a random number. And, a method for mobile terminal to authenticate communication network in the mobile communication network, at least includes the following process: set security key corresponding to mobile terminal in the mobile terminal; and after the authentication information from network equipment is received, the mobile terminal determines whether the network authentication succeeds based on said security key set by the mobile terminal and the authentication information.

Abstract: Transgenic plants with increased resistance to geminivirus infection, and nucleic acid constructs useful in producing such plants, are described. The transgenic plants express a mutant AL1/C1 geminivirus protein, which increases resistance to infection by at least one geminivirus, compared to a non-transformed control plant.

Abstract: Transgenic plants with increased resistance to geminivirus infection, and nucleic acid constructs useful in producing such plants, are described. The transgenic plants express a mutant AL1/C1 geminivirus protein, which increases resistance to infection by at least one geminivirus, compared to a non-transformed control plant.