Abstract: A genetically engineered microorganism is provided. The genetically engineered microorganism has a higher expression level of acid-tolerant gene than a source microorganism. The acid-tolerant gene includes at least one of dsdA, dcuC and glaA. A method of preparing the genetically engineered microorganism and a method of producing a target chemical using the genetically engineered microorganism are also provided.

Abstract: A p-aminobenzoic acid-producing microorganism is provided. The p-aminobenzoic acid-producing microorganism is obtained by a method for preparing a p-aminobenzoic acid-producing microorganism. The method for preparing a p-aminobenzoic acid-producing microorganism includes (a) performing an acclimation process on a source microorganism with at least one sulfonamide antibiotic to obtain at least one acclimatized microorganism and (b) screening out at least one p-aminobenzoic acid-producing microorganism from the at least one acclimatized microorganism, wherein the at least one p-aminobenzoic acid-producing microorganism has a higher p-aminobenzoic acid titer than the source microorganism.

Abstract: A dye for a material with amide functional groups and a dyeing method of using the same are provided. The dye includes a compound represented by Formula (I) and a solvent. Based on the total weight of the dye of 100 wt %, a content of the compound represented by Formula (I) is 40 wt % to 95 wt %. In Chemical Formula (I), R1, R2 and R3 are each independently H, —OH or —COOH, wherein at least one of R1, R2 and R3 is —OH or —COOH.

Abstract: A dyeing method includes immersing a fiber into a dye for dyeing the fiber, in which the dye includes indigo and indirubin, and the indigo and the indirubin have a weight ratio of 20:1 to 80:1. The indigo in the dye has a concentration of 0.1% o.w.f. to 5% o.w.f. The dyed fiber may simultaneously have high luminance, high color saturation, high strength of colorization, and sufficient colorfastness.

Abstract: The disclosure provides a dyeing method using bio-dye. A first compound containing an X group is reacted with a second compound containing a Y group to form a third compound, in which the X group is selected from the group consisting of an isocyanate group, a carbodiimide group, an aziridinyl group and an epoxy group, the Y group is selected from the group consisting of a hydroxyl group and an amine group, and the second compound is an amine compound and contains two or more Y groups. Cationization of the third compound is carried out with a fourth compound containing a carboxyl group to form a cationic modification agent. A cationic modification treatment is performed on a fiber material by using the cationic modification agent to form a cationized fiber material. Thereafter, a dyeing treatment is performed on the cationized fiber material through a dye solution containing bio-dye to color the cationized fiber material.

Abstract: A genetically modified microorganism includes: an exogenous nucleic acid sequence encoding naphthalene dioxygenase (NDO), wherein the endogenous icd gene of the genetically modified microorganism is knocked out, in which the endogenous icd gene encodes isocitrate dehydrogenase (IDH), and wherein the genetically modified microorganism is capable of using glutamic acid and/or a salt thereof as a nitrogen source to grow and producing indigo dye.

Abstract: A genetically modified microorganism includes: an exogenous nucleic acid sequence encoding naphthalene dioxygenase (NDO), wherein the endogenous icd gene of the genetically modified microorganism is knocked out, in which the endogenous icd gene encodes isocitrate dehydrogenase (IDH), and wherein the genetically modified microorganism is capable of using glutamic acid and/or a salt thereof as a nitrogen source to grow and producing indigo dye.

Abstract: The present invention provides a method for determining a survival rate and prognosis state and providing care for a patient having esophageal carcinoma, comprising: (a) providing a tissue sample from the patient; (b) measuring an expression level of a plurality of target genes comprising peptidase inhibitor 3 (PI3) and CD14 in the tissue sample from the patient using reagents specific for a gene product of the plurality of target genes that are selected from the group consisting of probes, primers, antibodies, and antibody fragments, wherein the expression level is measured by RNA expression or protein expression; (c) comparing the expression level of the plurality of target genes to a predetermined threshold indicative of a survival rate and prognosis state for the patient; and (d) providing care for the patient when the survival rate and prognosis state is worse, wherein the care is advising the patent to reduce the time between return visit or to add adjuvant chemotherapy after surgery.

Abstract: A convective polymerase chain reaction apparatus includes a tube, a temperature control unit, at least one light source and a sensor. The tube includes a cavity used to contain a reaction solution. The reaction solution has a liquid level measured from a bottom of the cavity to a top surface of the reaction solution. The temperature control unit is disposed adjacent to the tube for controlling the temperature of the reaction solution. The at least one light source provides a light beam passing through an incident portion of the tube to excite the reaction solution emitting a fluorescent light. The incident portion is located at a height greater than ½ of a liquid level. The sensor is adjacent to the tube for detecting the excited fluorescence. The light beam has an incident direction forming a non-straight angle with a long axis of the tube.

Abstract: A convective polymerase chain reaction apparatus includes a tube, a temperature control unit, at least one light source and a sensor. The tube includes a cavity used to contain a reaction solution. The reaction solution has a liquid level measured from a bottom of the cavity to a top surface of the reaction solution. The temperature control unit is disposed adjacent to the tube for controlling the temperature of the reaction solution. The at least one light source provides a light beam passing through an incident portion of the tube to excite the reaction solution emitting a fluorescent light. The incident portion is located at a height greater than ½ of a liquid level. The sensor is adjacent to the tube for detecting the excited fluorescence. The light beam has an incident direction forming a non-straight angle with a long axis of the tube.

Abstract: The present invention provides a method for determining a survival rate and prognosis state for a patient having esophageal carcinoma, comprising: (a) providing a tissue sample from the patient; (b) measuring a expression level of a target gene in the tissue sample from the patient using reagents specific for the target gene product that are selected from the group consisting of probes, primers, antibodies, and antibody fragments, wherein the expression level is RNA expression, a protein expression at a cellular level or a tissue level; (c) comparing the expression level to a predetermined threshold indicative of a survival rate and prognosis state for the patient; and (d) providing a survival rate and prognosis state for the patient so that care for the patient is able to be determined in accordance with the survival rate and prognosis state, wherein the target gene is peptidase inhibitor 3 (PI3).

Abstract: The present invention provides a method for predicting the survival rate and prognosis of esophageal carcinoma patients, which is characterized in examining the expression level of a specific gene, peptidase inhibitor 3 (PI3) or CD14 antigen (CD14) in a sample, and comparing to the average expression level of said specific gene from patients to determine the survival and prognosis status for esophageal cancer. The present invention further provides a kit for predicting the survival rate and prognosis of esophageal carcinoma patients.

Abstract: An automated microarray processing system includes a microarray housing assembly module, an incubation module, a washing module and at least one automated transport module. With the automated transport module being mechanically moved among the microarray housing assembly module, the incubation module and the washing module, biochemical reaction of a reaction region of a microarray and a biological sample solution disposed therebetween is automatically performed, and the reacted microarray is automatically cleaned when the biochemical reaction of the microarray is completed.

Abstract: An automated microarray processing system includes a microarray housing assembly module, an incubation module, a washing module and at least one automated transport module. With the automated transport module being mechanically moved among the microarray housing assembly module, the incubation module and the washing module, biochemical reaction of a reaction region of a microarray and a biological sample solution disposed therebetween is automatically performed, and the reacted microarray is automatically cleaned when the biochemical reaction of the microarray is completed.

Abstract: The present invention relates to a nucleic acid detection device. The present invention especially relates to a nucleic acid detection device utilizing the novel concept of multiple probes to one target. The device comprises a solid support, on which at least one probe set is attached. The probe set comprises multiple types of oligonucleotide probes, wherein each probe has a unique sequence complementary to the different target nucleic acid. The present invention also relates to a method for detecting nucleic acid. The method involves carrying out a hybridization reaction of the previously described device and a sample in order to determine the nucleic acids present in the sample.