Abstract: Disclosed are emulsion gel with both a freshness indication and a low migration rate, and a preparation method therefor. Lipase and organic acid are used and subjected to hydrophobic modification of anthocyanins to obtain acylated anthocyanins, the acylated anthocyanins and oil-phase gel are then dissolved in vegetable oil to form an oil phase, while a sodium alginate aqueous solution is used as a water phase, the oil phase and the water phase are mixed and subjected to high-speed shearing, and calcium carbonate and glucono-?-lactone are finally added for high-speed shearing to form emulsion gel. The emulsion gel prepared in the present disclosure has both a freshness indication and a low migration rate, and the preparation method of the present disclosure is simple, green, pollution-free and low energy consumption.

Abstract: The disclosure provides a modified cyclodextrin/mesoporous silica for adsorbing Pb and Cd and application thereof, belonging to the technical field of adsorbent materials. By using surface modification, chloroacetic acid is used as anions, and a modified cyclodextrin is grafted onto a surface of a cyclodextrin/mesoporous silica by nucleophilic substitution to prepare the modified cyclodextrin/mesoporous silica adsorbent material. The modified cyclodextrin/mesoporous silica adsorbent material prepared in the disclosure has the advantages of simple preparation method, strong adsorbability, easy separation, good biocompatibility and the like. When the material is used as an adsorbent to adsorb heavy metal ions Pb2+ and Cd2+, maximum removal rates can reach 97.8% and 81.29% respectively. Therefore, the material has wide application prospects in removal of heavy metals in sewage and the like, thereby improving the water environment that people depend on for survival.

Abstract: A nanozyme material and a preparation method thereof are provided. In the preparation method, Prussian blue (PB) is used to regulate the peroxidase activity of Fe,NA/CDs, and then gold nanoparticles are synthesized with Fe,NA/CDs as a reducing agent and a stabilizing agent. The nanozyme material has both a peroxidase activity and a Raman enhancement effect, which can be used for nanozyme-based surface-enhanced Raman spectroscopy (SERS). An embodiment of the present disclosure also provides a method for rapidly detection of a Pb2+ ion, which is based on a color change or an additional Raman SERS signal generation caused by nanozyme oxidation. Such sensing signals (colorimetric and SERS analysis) are derived from a catalytic oxidation reaction of ABTS or TMB, while the presence of Pb2+ specifically inhibits the oxidation, such that the rapid detection of Pb2+ can be achieved.

Abstract: Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86th site, the 373rd site, the 374th site, the 496th site, the 507th site, or the 747th site of agarase with an amino acid sequence as shown in SEQ ID NO. 1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.

Abstract: A visual loop-mediated isothermal amplification (LAMP) method for a rapid test of tobacco includes: extracting a genomic DNA of the tobacco, designing primers, establishing a LAMP reaction system, and optimizing the LAMP reaction system. The present disclosure designs the LAMP primers according to a conserved region of a screened tobacco-specific Ntsp151 genomic sequence, and establishes the LAMP method based on color determination. The present disclosure can rapidly identify flue-cured tobacco leaves, finished cigarettes, and tea cigarettes, and achieves a detection limit of 200 copies per reaction. The test results of the LAMP method for non-tobacco samples are negative, indicating that the LAMP method has high specificity. Compared with the quantitative polymerase chain reaction (qPCR) method, the LAMP method has a coincidence rate of 96.7%, but the LAMP method only needs about 1 h to obtain the results.

Abstract: A method for preparing V-type porous starch includes: mixing starch and a first ethanol solution in a reaction kettle, adding concentrated hydrochloric acid to the reaction kettle for acidity regulation, and controlling a temperature of a reaction system in the reaction kettle at 80-150° C.; cooling the reaction kettle, and adding a sodium hydroxide solution to the reaction system in the reaction kettle, until a pH of a mixture in the reaction kettle is neutral, to yield a starch solution; and centrifuging the starch solution, washing a collected precipitate with a second ethanol solution, leaching, drying, cooling, and pulverizing, to yield V-type porous starch.

Abstract: Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86th site, the 373rd site, the 374th site, the 496th site, the 507th site, or the 747th site of agarase with an amino acid sequence as shown in SEQ ID NO.1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.

Abstract: Disclosed is a method for purifying pea albumin PAla by using a negatively charged polysaccharide, which belongs to the field of protein extraction technology. According to the invention, with a natural edible negatively charged polysaccharide as a recycling material, based on the technical principle that the negatively charged polysaccharide preferentially binds to non-target proteins to precipitate and undergoes phase separation relative to the target protein, the separation and purification of pea albumin PAla are realized to prepare pure PAla. From the perspective of resource recycling, the invention can realize high value-added utilization of byproducts of pea protein isolate processing and prepare high-purity high-sulfur active protein components therefrom, thereby providing technical support for subsequent research.

Abstract: The present invention discloses an optimization method for a screen surface dynamic load of a vibrating screen. The method includes the following steps: step 1. selecting design variables, and establishing an experimental matrix; step 2. performing a response curved surface experiment; step 3. establishing two double-objective optimization models and solving the same to obtain two groups of Pareto solution sets, wherein the solution sets respectively represent screening efficiency optimization paths of the vibrating screen under the conditions of a high screen surface dynamic load and a low screen surface dynamic load; and step 4. calculating an optimization space for a screen surface dynamic load under a high screening efficiency. According to the method of the present invention, the screen surface dynamic load can be directly reduced, and the service life of the screen surface and the whole vibrating screen is prolonged.

Abstract: The present invention discloses an optimization method for a screen surface dynamic load of a vibrating screen. The method includes the following steps: step 1. selecting design variables, and establishing an experimental matrix; step 2. performing a response curved surface experiment; step 3. establishing two double-objective optimization models and solving the same to obtain two groups of Pareto solution sets, wherein the solution sets respectively represent screening efficiency optimization paths of the vibrating screen under the conditions of a high screen surface dynamic load and a low screen surface dynamic load; and step 4. calculating an optimization space for a screen surface dynamic load under a high screening efficiency. According to the method of the present invention, the screen surface dynamic load can be directly reduced, and the service life of the screen surface and the whole vibrating screen is prolonged.

Abstract: A method for preparing V-type granular porous starch includes the following steps: mixing starch and an ethanol aqueous solution in a temperature of 100-150° C. to yield V-type granular starch; and adding a mixed enzyme including alpha-amylase and amyloglucosidase to a mixture of the V-type granular starch and the ethanol aqueous solution, to enzymatically hydrolyze the V-type granular starch to yield V-type granular porous starch.

Abstract: The disclosure provides a modified cyclodextrin/mesoporous silica for adsorbing Pb and Cd and application thereof, belonging to the technical field of adsorbent materials. By using surface modification, chloroacetic acid is used as anions, and a modified cyclodextrin is grafted onto a surface of a cyclodextrin/mesoporous silica by nucleophilic substitution to prepare the modified cyclodextrin/mesoporous silica adsorbent material. The modified cyclodextrin/mesoporous silica adsorbent material prepared in the disclosure has the advantages of simple preparation method, strong adsorbability, easy separation, good biocompatibility and the like. When the material is used as an adsorbent to adsorb heavy metal ions Pb2+ and Cd2+, maximum removal rates can reach 97.8% and 81.29% respectively. Therefore, the material has wide application prospects in removal of heavy metals in sewage and the like, thereby improving the water environment that people depend on for survival.

Abstract: A method for screening adulteration of fibrate anti-hyperlipidemia chemicals in tea by combined method of high performance thin layer chromatography (HPTLC) and bioluminescence, which belongs to the field of food inspection. The method includes: firstly, formulating standard solutions of bezafibrate and ciprofibrate, and preparing a tea sample; pre-washing a thin-layer plate and then performing HPTLC spotting; performing HPTLC separation to move original mixed target objects mixed originally onto different positions of the thin-layer plate according to different molecular structures, so as to form a physical isolation; and subsequently, simultaneous detection of multiple targets in the tea sample could be realized conveniently through luminous bacteria coupled with the thin-layer plate by an immersed manner.

Abstract: A multi-process automatic machine system is configured to machine different surfaces of a workpiece. The multi-process automatic machine system includes at least two first robots, at least two first machining devices, and at least one transfer table positioned between the at least two first robots. Each first machining device is positioned adjacent to one of the at least two first robots. One of the at least two first robots is configured to translationally move the workpiece to the at least one transfer table after the first machining surface being machined. The at least one transfer table is configured to rotate the workpiece. Another one of the at least two first robots is configured to translationally move the workpiece from the at least one transfer table to the corresponding one of the at least two first machining device.

Abstract: A flexible torque-transferring apparatus includes a first end element, a second end element and a flexible connector for interconnecting the first and second end elements. The flexible connector includes a plurality of joints, each including a ball and a socket. The ball includes an end facet, a plurality of arched facets extending from the end facet, and a plurality of edges, each extending between two adjacent ones of the arched facets. The socket includes a cavity defined therein and a plurality of curved facets formed in the cavity. Each of the curved facets includes two ridges in contact with two portions of a corresponding one of the arched facets when the ball is placed in the socket.

Abstract: A computer implemented method and system for processing a client request for an application program. The application program may require one or more software applications as prerequisites. In response to a client request for the application program, the system automatically determines prerequisite application programs associated with the requested application program. The prerequisite applications include a first set of direct prerequisite application programs and a second set of indirect prerequisite application programs. The method includes checking whether the second set contains a duplicate prerequisite application program that corresponds to an original prerequisite application program existing in the first set, and removing the original prerequisite application program from the first set automatically if the second set contains the duplicate prerequisite application program. Further, the method includes saving non-duplicated prerequisite programs of the first set.