Abstract: The invention provides a method and a product for detecting the health status of an embryo by using a blastocyst culture medium. Specifically, the present invention provides an in vitro method for detecting the health status of embryos using blastocyst culture medium, characterized in that it comprises the steps of: (a) providing a first blastocyst culture system containing a blastocyst of in vitro culture on Day 5 (D5) to Day 6 (D6); (b) transferring the blastocysts into a second blastocyst culture system containing a fresh blastocyst culture medium, and culturing in the exchanged medium for a time of T1, thereby obtaining a spent blastocyst culture medium; (c) collecting the spent blastocyst culture medium, thereby obtaining a cell-free spent blastocyst culture medium, i.e. a test sample; and (d) performing a genetic assay to identify the health status of the blastocyst.