Abstract: The present disclosure relates to systems and methods for quantifying mechanical properties of a cell containing a nucleus and cytoplasm. The system comprises a microfluidic comprises a varying width configured to deform the cell to multiple deformation levels, an imaging device configured to obtain image data of the cell received by the microfluidic channel and a processor in communication with the imaging device. The processor is configured to receive, from the imaging device, image data of the cell deformed within the microfluidic channel at a first deformation level and a second deformation level different from the first deformation level and to determine, based on the image data, one or more parameters associated with the deformed cell at the first deformation level and the second deformation level.