Abstract: The present invention provides a high temperature resistant reverse transcriptase mutant and its use. In particular, the present invention constructs a reverse transcriptase (M-MLV) mutant library, and the mutants with improved thermostability and higher amplification efficiency are finally screened out by stepwise screening. Under high temperature conditions, the reverse transcription efficiency of the reverse transcriptase mutant of the present invention is greatly improved compared to the wild type.

Abstract: A communication system, method, and device for a miniature intelligent sensor. The communication system includes a sensor, a power gateway, and a power Internet of things (IoT) server. The sensor is configured to obtain sensor data and send the sensor data to the power gateway according to a Bluetooth communication protocol. The power gateway is configured to receive the sensor data and perform protocol conversion on the sensor data to obtain protocol conversion data adapted to a message queuing telemetry transport (MQTT) protocol. The power gateway is configured to send the protocol conversion data to the power IoT server according to the MQTT protocol. The power IoT server is configured to receive the protocol conversion data and obtain server receiving data of the power IoT server according to the protocol conversion data.