Abstract: A non-invasive, in vivo method for measuring glucose-mediated changes in tissue is disclosed. The method comprises the act of providing a system for directly or indirectly measuring impedance values. The method further comprises the act of providing at least three electrodes and corresponding electrode pads. The electrodes are connected to the system. The method further comprises the act of contacting the electrode pads to a user's skin. The method further comprises the act of contacting each of the at least four electrodes to a corresponding electrode pad. The method further comprises the act of applying an alternating current. The method further comprises the act of determining the correlation between the glucose concentration in the tissue and the measured changes in impedance.