Abstract: An improved method for myceliating coffee includes providing raw coffee beans, preparing the raw beans for fungal myceliation by removing chlorogenic acids from the green coffee beans inoculating the prepared raw coffee beans with a fungal component to enable fungal myceliation of the green coffee beans. The method includes buffering the aqueous solution with a buffer selected from the group consisting of: sodium chloride, citric acid and ascorbic acid. In one embodiment, the method further includes myceliating the raw coffee beans under optimal conditions for mycelial growth, and preparing the myceliated coffee beans for roasting by washing the myceliated coffee beans to remove undesired metabolites produced by the fungal component. The myceliated coffee beans are roasted. Roasted coffee beans are then ground and brewed into a coffee beverage.

Abstract: The present invention relates to a method of myceliation of agricultural substrates for producing functional foods with health benefits, wherein the method comprises of inoculating an agricultural substrate with one or more species of pure fungal culture comprising Basidiomycota and Ascomycota derived from liquid state fermentation, enabling mycelial growth on the agricultural substrate by controlling growth conditions and harvesting of a myceliated agricultural product after the mycelial growth reaches a desired stage. The present invention further provides a myceliated agricultural product enriched with nutrients, processed into a nutraceutical or functional food for human consumption.

Abstract: The present invention provides an aqueous extract of a myceliated agricultural substrate for use as a nutraceutical or food composition for human consumption comprising at least one small molecule compound, wherein mycelia is grown on an agricultural substrate by inoculating the agricultural substrate with a pure strain of a fungal culture selected from the group comprising Basidiomycota and Ascomycota derived from a liquid fermentation, under optimum growth conditions. The present invention further provides methods of formation of an aqueous extract and nutraceutical or food compositions obtained from the extract. The aqueous extract and the nutraceutical compositions obtained from the extract thereof have neuroprotective and neuroregenerative properties amongst many other therapeutic properties.