Abstract: The disclosure provides next generation sequencing-based methods and materials for detecting a gene copy number variant in a biological sample having one or more genes. The disclosure also provides an electronic computer system for detecting a gene copy number variant. Detection of gene copy number variants may be used to enable patients with increased risks associated with certain diseases to take preventative measures to reduce their risk or receive targeted treatment to improve their chances of survival.

Abstract: The disclosure provides methods for the personalized detection of the recurrence of a cancer or the metastasis of cancer in a subject. Such methods may comprise detecting one or more genetic mutations in a subject's cancer prior to treatment with an anti-cancer therapy and analyzing whether those one or more mutations are present in a liquid biopsy obtained from the subject after treatment with the anti-cancer therapy. Detection of the one or more mutations in the liquid biopsy may be used to assess that the cancer has recurred or metastasized in the subject, or evaluate the efficacy of a treatment.

Abstract: A disc style cross groove universal joint including an outer joint member open on front and back sides having a plurality of inward facing outer ball grooves and an inner joint member inside of the outer joint member having a plurality of outward facing inner ball grooves. The inner and outer ball grooves define crossed pairs having a bearing ball therebetween. In a center position of the inner joint member in the outer joint member, the bearing balls are evenly angularly spaced. A cage has a plurality of windows for the bearing balls. An outboard side edge of each cage window is separated from the center of the bearing ball therein by a long span dimension in the center position of the inner joint member. An inboard side edge of each cage window is separated from the center of the bearing ball therein by a short span dimension in the center position of the inner joint member.