Abstract: Provided herein are methods of differentiating neural cells as well as methods of predicting cell engraftment of populations of cells using gene expression, for instance populations of neuronal progenitor cells, following implantation in a subject. Also provided herein are related compositions, articles of manufacture, and kits, including for use in methods of treating a subject having neurodegenerative disease, for instance Parkinson's disease.

Abstract: Provided herein are methods for classifying the differentiation state of an in vitro population of cells, for instance an in vitro population of neuronal cells, as well as methods for selecting and/or implanting an in vitro population of cells having a desired differentiation state. Also provided herein are computing devices for performing the provided methods as well as related compositions, articles of manufacture, and kits, including for use in methods of treating a subject having a disease or condition, such as a neurodegenerative disease, for instance Parkinson's disease.

Abstract: The present disclosure provides methods of lineage specific differentiation of pluripotent stem cells, including induced pluripotent stem cells, into floor plate midbrain progenitor cells, determined dopamine (DA) neuron progenitor cells, and/or DA neurons. Also provided are compositions uses thereof, such as for treating neurodegenerative diseases and conditions, including Parkinson's disease.