Patents by Inventor Jim Przybytek

Jim Przybytek has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Synthesis of oligonucleotides or phosphorothioate oligonucleotide with a capping agent of N-methylimidazole free of 1,3,5-trimethylhexahydro-1,3,5-triazine
    Patent number: 8039612
    Abstract: According to the present invention, there is provided a process for making an oligonucleotide or a phosphorothioate oligonucleotide. The process has the following steps: (a) providing an amount of a blocked nucleotide; (b) deblocking the blocked nucleotide to form an unblocked nucleotide; (c) activating the deblocked nucleotide; (d) coupling the deblocked nucleotide with a phosphoramidite to form a phosphite oligomer; (e) capping any uncoupled deblocked nucleotide via reaction with an amount of acetic anhydride and an amount of N-methylimidazole that is substantially free of 1,3,5-trimethylhexahydro-1,3,5-triazine; (f) oxidizing the phosphite oligomer to form the oligonucleotide or sulfurizing the phosphite oligomer to form a phosphorothioate oligonucleotide; and (g) optionally repeating steps (b) through (f). There is also a process for capping a nucleotide.
    Type: Grant
    Filed: October 10, 2008
    Date of Patent: October 18, 2011
    Assignee: Honeywell International, Inc.
    Inventors: Sandra Lorenz, Jim Przybytek, Karel Snoble
  • SYNTHESIS OF OLIGONUCLEOTIDES OR PHOSPHOROTHIOATE OLIGONUCLEOTIDE WITH A CAPPING AGENT OF N-METHYLIMIDAZOLE FREE OF 1,3,5-TRIMETHYLHEXAHYDRO-1,3,5-TRIAZINE
    Publication number: 20090099352
    Abstract: According to the present invention, there is provided a process for making an oligonucleotide or a phosphorothioate oligonucleotide. The process has the following steps: (a) providing an amount of a blocked nucleotide; (b) deblocking the blocked nucleotide to form an unblocked nucleotide; (c) activating the deblocked nucleotide; (d) coupling the deblocked nucleotide with a phosphoramidite to form a phosphite oligomer; (e) capping any uncoupled deblocked nucleotide via reaction with an amount of acetic anhydride and an amount of N-methylimidazole that is substantially free of 1,3,5-trimethylhexahydro-1,3,5-triazine; (f) oxidizing the phosphite oligomer to form the oligonucleotide or sulfurizing the phosphite oligomer to form a phosphorothioate oligonucleotide; and (g) optionally repeating steps (b) through (f). There is also a process for capping a nucleotide.
    Type: Application
    Filed: October 10, 2008
    Publication date: April 16, 2009
    Inventors: SANDRA LORENZ, Jim Przybytek, Karel Snoble