Abstract: There is provided a biomolecule-immobilized plate having a flat part, at least a surface of the flat part comprising a polymer material, wherein the flat part has an arithmetic average roughness of 0.1 nm˜5 nm, and a portion of the flat part has functional groups. Further, the biomolecule-immobilized plate is fabricated by irradiating the flat part of the plate surface which comprises the polymer material, with an ultraviolet ray under ozone atmosphere to produce functional groups having bonding ability to the biomolecules on a portion of the flat part. Thereby, it is possible to provide a biomolecule-immobilized plate having a surface of a high degree of smoothness on which functional groups are produced, a biomolecule-immobilized plate on which biomolecules are immobilized via functional groups, and methods for fabricating these biomolecule-immobilized plates easily and inexpensively.

Abstract: There is provided a biomolecule-immobilized plate having a flat part, at least a surface of the flat part comprising a polymer material, wherein the flat part has an arithmetic average roughness of 0.1 nm˜5 nm, and a portion of the flat part has functional groups. Further, the biomolecule-immobilized plate is fabricated by irradiating the flat part of the plate surface which comprises the polymer material, with an ultraviolet ray under ozone atmosphere to produce functional groups having bonding ability to the biomolecules on a portion of the flat part. Thereby, it is possible to provide a biomolecule-immobilized plate having a surface of a high degree of smoothness on which functional groups are produced, a biomolecule-immobilized plate on which biomolecules are immobilized via functional groups, and methods for fabricating these biomolecule-immobilized plates easily and inexpensively.

Abstract: On a substrate 41 holding a sample to be detected, a dielectric multilayer 42 is disposed which reflects excitation light e1 supplied from above the substrate 41 and transmits fluorescence f1 emitted from the sample, and the excitation light e1 is reflected at the dielectric multilayer 42 while the transmitted fluorescence f1 is detected by a light receiving unit 44, thereby providing a fluorescence measurement apparatus which can resolve a problem of reduction in detection sensitivity due to autofluorescence from the substrate or leakage of the excitation light from a light receiving filter, and which can detect the sample with high sensitivity.

Abstract: There is provided a biomolecule-immobilized plate having a flat part, at least a surface of the flat part comprising a polymer material, wherein the flat part has an arithmetic average roughness of 0.1 nm˜5 nm, and a portion of the flat part has functional groups. Further, the biomolecule-immobilized plate is fabricated by irradiating the flat part of the plate surface which comprises the polymer material, with an ultraviolet ray under ozone atmosphere to produce functional groups having bonding ability to the biomolecules on a portion of the flat part. Thereby, it is possible to provide a biomolecule-immobilized plate having a surface of a high degree of smoothness on which functional groups are produced, a biomolecule-immobilized plate on which biomolecules are immobilized via functional groups, and methods for fabricating these biomolecule-immobilized plates easily and inexpensively.

Abstract: There is disposed, on a substrate 41 holding a sample to be detected, a dielectric multilayer 42 which reflects excitation light e1 supplied from above the substrate 41 and transmits fluorescence f1 emitted from the sample, and the excitation light e1 is reflected at the dielectric multilayer 42 while the transmitted fluorescence f1 is detected by a light receiving unit 44, thereby providing a fluorescence measurement apparatus which can resolve a problem of reduction in detection sensitivity due to autofluorescence from the substrate or leakage of the excitation light from a light receiving filter, and which can detect the sample with high sensitivity.