Abstract: The present invention relates to a method for preparing triple-coated lactic acid bacteria, triple-coated lactic acid bacteria prepared thereby, and an article comprising the same. More particularly, the present invention relates to a method for preparing lactic acid bacteria triple-coated with protein, polysaccharide and nanoparticles, triple-coated lactic acid bacteria prepared thereby, and an article comprising the same. The triple-coated lactic acid bacteria prepared according to the present invention provides have better acid resistance, bile resistance and stability in accelerated test than conventional double-coated lactic acid bacteria. They are not killed by gastric acid or bile acid upon uptake, and maintain their inherent physiological activities, and, therefore, they can be effectively used in various products, including fermented milk, processed milk, fermented soybean products, fermented kimchi products, functional drinks, functional foods, conventional foods, cosmetics, and the like.