Abstract: Provided is a test kit for conducting a membrane sandwich immunoassay of a multivalent antigen, optionally including reagents, the test kit includes a hollow container for reception of a fluid specimen, containing in series fluid absorbent body, a fluid pervious layer, and a fluid pervious solid membrane layer on which antibody is immobilized such that fluid added (e.g., labeled antibody and fluid specimen) to the container migrates through the membrane and in the presence of unlabeled scavenger antibody on the reactive surface of the membrane forms a multiple-site immunospecific antibody-antigen-antibody sandwich immobilized and labeled thereon, preferably in the form of a visible positive or negative indicator of the presence and/or amount of the analyte antigen in the specimen sample. The scavenger antibody serves to prevent false positive or other spurious reactions due to antigen analogs, antigen interference substances, or circulating human immnoglobulins.

Abstract: A method and reagent kit means are provided for assay of a selected antigen such as hCG or CEA in an aliquot of body fluid. The method comprises the steps of constituting the aliquot in a mixture comprising tracer (which may be an enzyme tracer or a radioactive tracer) conjugated with monoclonal antibody, and separate immobilized monoclonal antibody, incubating the mixture to enable separation of a solid phase antigen antibody conjugate in sandwich relation, and measuring the tracer content and corresponding antigen content of the aqueous phase or the solid phase. The antibody (conjugated and/or immobilized) comprises multiple monoclonal antibodies from different cell lines so that the specificity of the assay is enhanced, and the possibility of unrecognized antigen fragments is reduced.

Abstract: A method is provided for determining the percentage of glycohemoglobin in patient whole blood. The method comprises subjecting total hemoglobin comprising non-carbohydrate fixed hemoglobin and glycohemoglobin of samples of (1) patient whole blood lysate P and (2) whole blood lysates K.sub.1 and K.sub.2 of known glycohemoglobin percentages %.sub.1 and %.sub.2, to batchwise selective adsorption on an adsorbent which is specific either for the hemoglobin or for the glycohemoglobin, thereby adsorbing each sample under the same conditions and leaving in solution the corresponding non-adsorbed total hemoglobin fractions P.sub.f, K.sub.1f and K.sub.2f. The ratios R.sub.p, R.sub.k1 and R.sub.k2 of the optical density of the total hemoglobin fractions P.sub.f, K.sub.1f and K.sub.2f to the respective optical density of total hemoglobin aliquot samples of lysates P, K.sub.1 and K.sub.2 are measured, and the percentage of glycohemoglobin relative to total hemoglobin for each patient blood sample is calculated.

Abstract: Novel activated polyamide polymer container or test tube means and a method for the determination of ligand or analyte content in aqueous samples, are provided. The surfaces of the container tubes are specially activated thermally during their manufacture by injection molding thereby avoiding the need for subsequent coating of the tubes, e.g., with antibody, for binding assay procedures.

Abstract: A method and reagent kit means are provided for assay of a selected hapten in an aliquot of body fluid containing lipid. The method comprises the steps of constituting the aliquot in a mixture with surfactant and incubating the mixture to solubilize the same. The content of hapten in the incubated mixture is taken up selectively by hapten-specific antibody and read by hapten non-lipid conjugate tracer assay.