Abstract: The present disclosure provides a fluorescence image registration method, the method includes: acquiring a fluorescence image of a biochip; selecting a preset local region of the fluorescence image; acquiring a position of a minimum value of a sum of brightness values of pixels in a first direction and a second direction, and obtaining pixel-level registration points; dividing the pixel-level registration points into non-defective pixels and defective pixels; if the fluorescence image meets the preset standard, correcting positions of the defective pixels according to positions of the non-defective pixels; acquiring a position of a center of gravity of image points of fluorescent molecules according to a center of gravity method; fitting straight lines in the first direction and the second direction respectively according to the position of the center of gravity; and acquiring boundary points of the fluorescence image and calculating the positions of the boundary points.

Abstract: A multimedia broadcasting system triggered by a charging operation includes a multimedia device and a power transmission device. The power transmission device includes a discharging connector and a control unit. When the multimedia device is connected to the discharging connector, the control unit transmits readable identification information to the multimedia device via the discharging connector to trigger the multimedia device to play digital content data corresponding to the identification information. Thus, by triggering the multimedia to play advertisements or music via a charging operation, the present invention achieves a novel multimedia broadcasting method.

Abstract: A porous carbonized substrate and its preparation method and uses are provided. The porous carbonized substrate has an oxygen content ranging from about 1 wt % to about 13 wt % and a nitrogen content ranging from about 2 wt % to about 16 wt %, based on the total weight of the substrate. The porous carbonized substrate can be prepared by a method comprising the following steps: providing a fiber substrate containing one or more oxidized fibers, one or more polyamide fibers or a mixture thereof; and thermally treating the fiber substrate under an inert gas atmosphere, wherein the thermally treating step comprises putting the fiber substrate in the inert gas atmosphere and increasing the temperature of the inert gas atmosphere to an elevated temperature ranging from about 700° C. to about 2000° C. with a rate of from about 50° C./minute to about 300° C./minute. The porous carbonized substrate is used as a gas diffusion layer of a fuel cell.