Abstract: The disclosure relates to a hydropower device with its operation driven by a combination of lift and drag forces. The device comprises a shaft, a lift-type runner, a first-stage drag-type runner, and a second-stage drag-type runner, wherein the lift-type runner, the first-stage drag-type runner, and the second-stage drag-type runner are coaxially installed on the shaft. The shaft is rotatably installed on a supporting frame. The first-stage drag-type runner and the second-stage drag-type runner are mounted in series on the shaft. A disc generator is installed on the shaft and meanwhile in the middle of the first- and the second-stage drag-type runners. A lift-type runner is connected to the shaft through struts, and the blades of the lift-type runner are oriented in the periphery of the first-type and the second-type runners.

Abstract: The disclosure relates to a hydropower equipment with graded utilization of kinetic energy of flowing water, including three steps on the inclined surface of the triangular base from top to bottom, wherein support rods are provided on the vertical surface of each step, runner one, runner two and runner three arranged in sequence from high to low are respectively and rotatably supported on the corresponding support rods through the shaft, all the three shafts are horizontally arranged and connected to the corresponding shaft of the generator; horizontal flow channels are set inside the lower part of the triangular base for guiding water to flow toward downstream direction, and the outlets of the horizontal flow channels directly face runner three.

Abstract: The present invention relates to the technical field of microorganisms, and specifically to engineered microorganisms expressing acetoacetyl-CoA reductase variants and methods for increasing the proportion of 3-hydroxyhexanoic acid in PHA. The acetoacetyl-CoA reductase variants and their coding genes provided by the present invention can significantly increase the proportion of 3-hydroxyhexanoic acid in PHA produced by strains; the proportion of 3-hydroxyhexanoic acid in PHA produced by the engineered Ralstonia eutropha constructed utilizing the acetoacetyl-CoA reductase variants and their coding genes provided by the present invention is significantly increased, which provides new genes and strain resources for strains producing poly(3-hydroxybutyrate-co-3-10 the development of engineered hydroxyhexanoate) with high proportion of 3-hydroxyhexanoic acid.

Abstract: Disclosed herein are methods that have been developed to control the formation of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess. Also disclosed are protein solution parameters that allow for controlling the formation of disulfide bonds. In one example, the methods disclosed herein can be used to control the proportion of half antibody molecules in an antibody solution.

Abstract: A recombinant bacterium with a high PHA yield and its construction method provide an engineered microorganism that can be used for the production of polyhydroxyalkanoate (PHA). A recombinant Ralstonia eutropha with a high PHA yield includes a promoter for upregulating a phaJ gene and further includes a mutant phaC gene. The recombinant Ralstonia eutropha can be used for producing 3-hydroxybutyrate-co-3-hydroxyhexanoate (PHBHHx) in PHA.

Abstract: The present invention relates to the technical field of microorganisms, and specifically to engineered microorganisms expressing acetoacetyl-CoA reductase variants and methods for increasing the proportion of 3-hydroxyhexanoic acid in PHA. The acetoacetyl-CoA reductase variants and their coding genes provided by the present invention can significantly increase the proportion of 3-hydroxyhexanoic acid in PHA produced by strains; the proportion of 3-hydroxyhexanoic acid in PHA produced by the engineered Ralstonia eutropha constructed utilizing the acetoacetyl-CoA reductase variants and their coding genes provided by the present invention is significantly increased, which provides new genes and strain resources for strains producing poly(3-hydroxybutyrate-co-3-10 the development of engineered hydroxyhexanoate) with high proportion of 3-hydroxyhexanoic acid.

Abstract: Provided is engineered microorganisms expressing acetoacetyl-CoA reductase variants and a method for improving the yield of PHA. Compared with the wild-type acetoacetyl-CoA reductase represented by SEQ ID NO. 31, the variant has one or more of the following mutations: (1) mutation of valine at position 141 to isoleucine or leucine; (2) mutation of methionine at position 12 to threonine, serine, alanine, leucine, lysine or isoleucine; (3) mutation of isoleucine at position 194 to valine, leucine or methionine; (4) mutation of glutamic acid at position 42 to lysine, glutamine, leucine, aspartic acid, proline, threonine, asparagine, or histidine; and (5) mutation of phenylalanine at position 55 to valine, alanine or isoleucine. The variants and their coding genes can promote the synthesis and accumulation of PHA by the strain and increase the yield of PHA.

Abstract: Provided is engineered microorganisms expressing acetoacetyl-CoA reductase variants and a method for improving the yield of PHA. Compared with the wild-type acetoacetyl-CoA reductase represented by SEQ ID NO. 31, the variant has one or more of the following mutations: (1) mutation of valine at position 141 to isoleucine or leucine; (2) mutation of methionine at position 12 to threonine, serine, alanine, leucine, lysine or isoleucine; (3) mutation of isoleucine at position 194 to valine, leucine or methionine; (4) mutation of glutamic acid at position 42 to lysine, glutamine, leucine, aspartic acid, proline, threonine, asparagine, or histidine; and (5) mutation of phenylalanine at position 55 to valine, alanine or isoleucine. The variants and their coding genes can promote the synthesis and accumulation of PHA by the strain and increase the yield of PHA.

Abstract: A recombinant bacterium with a high PHA yield and its construction method provide an engineered microorganism that can be used for the production of polyhydroxyalkanoate (PHA). A recombinant Ralstonia eutropha with a high PHA yield includes a promoter for upregulating a phaJ gene and further includes a mutant phaC gene. The recombinant Ralstonia eutropha can be used for producing 3-hydroxybutyrate-co-3-hydroxyhexanoate (PHBHHx) in PHA.

Abstract: A single piece-type bipolar film roll with a mesh cloth support and a manufacturing method therefor. The single piece-type bipolar film roll is supported by a high-strength ultra-high molecular weight polyethylene mesh cloth, one side of the single piece-type bipolar film roll is a cation exchange layer containing a benzenesulfonic acid group, the other side of the single piece-type bipolar roll is an anion exchange layer containing a benzyl dimethyl butyl ammonium quaternary amino group, and the middle is a water dissociation catalyst layer containing a benzyl methyl butyl amine tertiary amino group, and the three layers form the single piece-type bipolar film roll.

Abstract: A computer-implemented method, according to one embodiment, is for integrating magnetic tape storage with a distributed disk file system. The computer-implemented method includes: registering for a subset of data operations that are recorded at a central location, where the central location is coupled to a plurality of distributed accessor nodes. A subset of available resources in one or more magnetic tape libraries that are coupled to the plurality of distributed accessor nodes are registered for and further managed. Moreover, a performance of the subset of data operations using the registered subset of available resources is scheduled. Supplemental data operations which correspond to the subset of data operations are also automatically received from the central location.

Abstract: A method includes determining dimensions of a web browser presenting a webpage, the webpage including an inner container and an outer container. The method modifies dimensions of the outer container of the webpage based upon an associated maximum height value, an associated minimum height value, and an amount of data within the outer container. Responsive to calculating a vertical height of the inner container, assigning a maximum height value and a minimum height value to the inner container. The method modifies dimensions of the inner container to a minimum height for the web browser to present the data of the webpage, based on the assigned maximum height value and minimum height value of the inner container. The method augments the web browser to include a scrollbar based on the modified dimensions of the outer container and the modified dimensions of the inner container.

Abstract: Provided herein are methods of processing a cell culture and open circuit filtration systems.

Abstract: The present disclosure provides, among other things, continuous culture methods for producing a cell product, e.g., a recombinant protein, e.g., a glycoprotein, e.g., an antibody agent or a fusion protein. In some instances, methods herein allow large-scale production of a recombinant protein using continuous culture methods. The present disclosure identifies and addresses a problem with current continuous cell culture techniques in that at large-scale culture of certain cells have insufficient viable cell concentrations and impaired cell viability. The present disclosure provides, in part, methods and systems for large-scale continuous culture of shear-sensitive cells.

Abstract: A computer-implemented method, according to one embodiment, is for integrating magnetic tape storage with a distributed disk file system. The computer-implemented method includes: registering for a subset of data operations that are recorded at a central location, where the central location is coupled to a plurality of distributed accessor nodes. A subset of available resources in one or more magnetic tape libraries that are coupled to the plurality of distributed accessor nodes are registered for and further managed. Moreover, a performance of the subset of data operations using the registered subset of available resources is scheduled. Supplemental data operations which correspond to the subset of data operations are also automatically received from the central location.

Abstract: Disclosed herein are methods that have been developed to control the formation of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess. Also disclosed are protein solution parameters that allow for controlling the formation of disulfide bonds. In one example, the methods disclosed herein can be used to control the proportion of half antibody molecules in an antibody solution.

Abstract: Disclosed herein are methods that have been developed to control the formation of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess. Also disclosed are protein solution parameters that allow for controlling the formation of disulfide bonds. In one example, the methods disclosed herein can be used to control the proportion of half antibody molecules in an antibody solution.

Abstract: A method includes determining dimensions of a web browser presenting a webpage, the webpage including an inner container and an outer container. The method modifies dimensions of the outer container of the webpage based upon an associated maximum height value, an associated minimum height value, and an amount of data within the outer container. Responsive to calculating a vertical height of the inner container, assigning a maximum height value and a minimum height value to the inner container. The method modifies dimensions of the inner container to a minimum height for the web browser to present the data of the webpage, based on the assigned maximum height value and minimum height value of the inner container. The method augments the web browser to include a scrollbar based on the modified dimensions of the outer container and the modified dimensions of the inner container.

Abstract: A method includes determining dimensions of a web browser presenting a webpage, the webpage including an inner container and an outer container. The method modifies dimensions of the outer container of the webpage based upon an associated maximum height value, an associated minimum height value, and an amount of data within the outer container. Responsive to calculating a vertical height of the inner container, assigning a maximum height value and a minimum height value to the inner container. The method modifies dimensions of the inner container to a minimum height for the web browser to present the data of the webpage, based on the assigned maximum height value and minimum height value of the inner container. The method augments the web browser to include a scrollbar based on the modified dimensions of the outer container and the modified dimensions of the inner container.

Abstract: Disclosed herein are methods that have been developed to control the formation of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess. Also disclosed are protein solution parameters that allow for controlling the formation of disulfide bonds. In one example, the methods disclosed herein can be used to control the proportion of half antibody molecules in an antibody solution.