Abstract: A soft-switching power converter includes a main switch, an energy-releasing switch, and an inductive coupled unit. The main switch is a controllable switch. The energy-releasing switch is coupled to the main switch. The inductive coupled unit is coupled to the main switch and the energy-releasing switch. The inductive coupled unit includes a first inductance, a second inductance coupled to the first inductance, and an auxiliary switch unit. The auxiliary switch unit is coupled to the second inductance to form a closed loop. The main switch and the energy-releasing switch are alternately turned on and turned off. The auxiliary switch unit is controlled to start turning on before the main switch is turned on so as to provide at least one current path.

Abstract: A material conveying device includes a base frame, a first conveying unit, a second conveying unit and a transfer assembly. The first conveying unit is provided on the base frame, the second conveying unit is provided on the base frame, and the transfer assembly is provided on the base frame and can transfer a material between the first conveying unit and the second conveying unit. The present disclosure further provides a processing equipment facilitating material distribution and a material distribution method.

Abstract: Provided is engineered microorganisms expressing acetoacetyl-CoA reductase variants and a method for improving the yield of PHA. Compared with the wild-type acetoacetyl-CoA reductase represented by SEQ ID NO. 31, the variant has one or more of the following mutations: (1) mutation of valine at position 141 to isoleucine or leucine; (2) mutation of methionine at position 12 to threonine, serine, alanine, leucine, lysine or isoleucine; (3) mutation of isoleucine at position 194 to valine, leucine or methionine; (4) mutation of glutamic acid at position 42 to lysine, glutamine, leucine, aspartic acid, proline, threonine, asparagine, or histidine; and (5) mutation of phenylalanine at position 55 to valine, alanine or isoleucine. The variants and their coding genes can promote the synthesis and accumulation of PHA by the strain and increase the yield of PHA.

Abstract: Provided is engineered microorganisms expressing acetoacetyl-CoA reductase variants and a method for improving the yield of PHA. Compared with the wild-type acetoacetyl-CoA reductase represented by SEQ ID NO. 31, the variant has one or more of the following mutations: (1) mutation of valine at position 141 to isoleucine or leucine; (2) mutation of methionine at position 12 to threonine, serine, alanine, leucine, lysine or isoleucine; (3) mutation of isoleucine at position 194 to valine, leucine or methionine; (4) mutation of glutamic acid at position 42 to lysine, glutamine, leucine, aspartic acid, proline, threonine, asparagine, or histidine; and (5) mutation of phenylalanine at position 55 to valine, alanine or isoleucine. The variants and their coding genes can promote the synthesis and accumulation of PHA by the strain and increase the yield of PHA.

Abstract: A recombinant bacterium with a high PHA yield and its construction method provide an engineered microorganism that can be used for the production of polyhydroxyalkanoate (PHA). A recombinant Ralstonia eutropha with a high PHA yield includes a promoter for upregulating a phaJ gene and further includes a mutant phaC gene. The recombinant Ralstonia eutropha can be used for producing 3-hydroxybutyrate-co-3-hydroxyhexanoate (PHBHHx) in PHA.

Abstract: A single piece-type bipolar film roll with a mesh cloth support and a manufacturing method therefor. The single piece-type bipolar film roll is supported by a high-strength ultra-high molecular weight polyethylene mesh cloth, one side of the single piece-type bipolar film roll is a cation exchange layer containing a benzenesulfonic acid group, the other side of the single piece-type bipolar roll is an anion exchange layer containing a benzyl dimethyl butyl ammonium quaternary amino group, and the middle is a water dissociation catalyst layer containing a benzyl methyl butyl amine tertiary amino group, and the three layers form the single piece-type bipolar film roll.

Abstract: A computer-implemented method, according to one embodiment, is for integrating magnetic tape storage with a distributed disk file system. The computer-implemented method includes: registering for a subset of data operations that are recorded at a central location, where the central location is coupled to a plurality of distributed accessor nodes. A subset of available resources in one or more magnetic tape libraries that are coupled to the plurality of distributed accessor nodes are registered for and further managed. Moreover, a performance of the subset of data operations using the registered subset of available resources is scheduled. Supplemental data operations which correspond to the subset of data operations are also automatically received from the central location.

Abstract: A method includes determining dimensions of a web browser presenting a webpage, the webpage including an inner container and an outer container. The method modifies dimensions of the outer container of the webpage based upon an associated maximum height value, an associated minimum height value, and an amount of data within the outer container. Responsive to calculating a vertical height of the inner container, assigning a maximum height value and a minimum height value to the inner container. The method modifies dimensions of the inner container to a minimum height for the web browser to present the data of the webpage, based on the assigned maximum height value and minimum height value of the inner container. The method augments the web browser to include a scrollbar based on the modified dimensions of the outer container and the modified dimensions of the inner container.

Abstract: Provided herein are methods of processing a cell culture and open circuit filtration systems.

Abstract: The present disclosure provides, among other things, continuous culture methods for producing a cell product, e.g., a recombinant protein, e.g., a glycoprotein, e.g., an antibody agent or a fusion protein. In some instances, methods herein allow large-scale production of a recombinant protein using continuous culture methods. The present disclosure identifies and addresses a problem with current continuous cell culture techniques in that at large-scale culture of certain cells have insufficient viable cell concentrations and impaired cell viability. The present disclosure provides, in part, methods and systems for large-scale continuous culture of shear-sensitive cells.

Abstract: A computer-implemented method, according to one embodiment, is for integrating magnetic tape storage with a distributed disk file system. The computer-implemented method includes: registering for a subset of data operations that are recorded at a central location, where the central location is coupled to a plurality of distributed accessor nodes. A subset of available resources in one or more magnetic tape libraries that are coupled to the plurality of distributed accessor nodes are registered for and further managed. Moreover, a performance of the subset of data operations using the registered subset of available resources is scheduled. Supplemental data operations which correspond to the subset of data operations are also automatically received from the central location.

Abstract: Disclosed herein are methods that have been developed to control the formation of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess. Also disclosed are protein solution parameters that allow for controlling the formation of disulfide bonds. In one example, the methods disclosed herein can be used to control the proportion of half antibody molecules in an antibody solution.

Abstract: Disclosed herein are methods that have been developed to control the formation of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess. Also disclosed are protein solution parameters that allow for controlling the formation of disulfide bonds. In one example, the methods disclosed herein can be used to control the proportion of half antibody molecules in an antibody solution.

Abstract: A method includes determining dimensions of a web browser presenting a webpage, the webpage including an inner container and an outer container. The method modifies dimensions of the outer container of the webpage based upon an associated maximum height value, an associated minimum height value, and an amount of data within the outer container. Responsive to calculating a vertical height of the inner container, assigning a maximum height value and a minimum height value to the inner container. The method modifies dimensions of the inner container to a minimum height for the web browser to present the data of the webpage, based on the assigned maximum height value and minimum height value of the inner container. The method augments the web browser to include a scrollbar based on the modified dimensions of the outer container and the modified dimensions of the inner container.

Abstract: A method includes determining dimensions of a web browser presenting a webpage, the webpage including an inner container and an outer container. The method modifies dimensions of the outer container of the webpage based upon an associated maximum height value, an associated minimum height value, and an amount of data within the outer container. Responsive to calculating a vertical height of the inner container, assigning a maximum height value and a minimum height value to the inner container. The method modifies dimensions of the inner container to a minimum height for the web browser to present the data of the webpage, based on the assigned maximum height value and minimum height value of the inner container. The method augments the web browser to include a scrollbar based on the modified dimensions of the outer container and the modified dimensions of the inner container.

Abstract: Disclosed herein are methods that have been developed to control the formation of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess. Also disclosed are protein solution parameters that allow for controlling the formation of disulfide bonds. In one example, the methods disclosed herein can be used to control the proportion of half antibody molecules in an antibody solution.

Abstract: Provided herein are methods of processing a cell culture and open circuit filtration systems.

Abstract: One aspect of the present invention relates to magnetic nanoparticles colloidally stabilized in aqueous milieu by association with an organic phase. The organic phase may be either a fluorinated polymer or an organic hydrocarbon bilayer, wherein the two layers are chemically bonded to each other. The stabilized particles are further non-toxic and provide useful enhancements in bioprocesses. Another aspect of the present invention relates to compositions comprising an oxygen-dissolving fluid vehicle and surface modified, nanometer-sized magnetic particles. The inventive compositions have utility in a wide range of applications, but are particularly suitable for use as recyclable oxygen carriers, separation and purification vehicles, and bioprocessing media, including fermentation processes.

Abstract: One aspect of the present invention relates to magnetic nanoparticles colloidally stabilized in aqueous milieu by association with an organic phase. The organic phase may be either a fluorinated polymer or an organic hydrocarbon bilayer, wherein the two layers are chemically bonded to each other. The stabilized particles are further non-toxic and provide useful enhancements in bioprocesses. Another aspect of the present invention relates to compositions comprising an oxygen-dissolving fluid vehicle and surface modified, nanometer-sized magnetic particles. The inventive compositions have utility in a wide range of applications, but are particularly suitable for use as recyclable oxygen carriers, separation and purification vehicles, and bioprocessing media, including fermentation processes.