Abstract: The present disclosure provides a fluorescence image registration method, the method includes: acquiring a fluorescence image of a biochip; selecting a preset local region of the fluorescence image; acquiring a position of a minimum value of a sum of brightness values of pixels in a first direction and a second direction, and obtaining pixel-level registration points; dividing the pixel-level registration points into non-defective pixels and defective pixels; if the fluorescence image meets the preset standard, correcting positions of the defective pixels according to positions of the non-defective pixels; acquiring a position of a center of gravity of image points of fluorescent molecules according to a center of gravity method; fitting straight lines in the first direction and the second direction respectively according to the position of the center of gravity; and acquiring boundary points of the fluorescence image and calculating the positions of the boundary points.

Abstract: A thermal spraying method is provided for manufacturing anti-sliding plates. The method uses a mask located upon a plate with a specific height. The mask has plural openings of a specific shape. When a thermal spraying device thermal-sprays thread material from the upper side of the mask, melted thread material passes through the openings to form anti-sliding spots of a specific shape on the plate. By means of thermal spraying on the mask with the openings, an anti-sliding plate with high roughness is efficiently produced.