Abstract: Proposed by the present disclosure are an FPGA-based resequencing analysis method and device, wherein the method comprises: receiving genomic resequencing data; using the resequencing data as an input of an FPGA, determining a comparison result in the resequencing process according to an output of the FPGA, and simultaneously performing sorting and deduplication processing on the comparison result; correcting a base quality value of the comparison result after sorting and deduplication processing; and detecting a mutation result according to the corrected comparison result. The described method may save program running time, save calculation costs, and improve resequencing efficiency.

Abstract: Proposed by the present disclosure are an FPGA-based resequencing analysis method and device, wherein the method comprises: receiving genomic resequencing data; using the resequencing data as an input of an FPGA, determining a comparison result in the resequencing process according to an output of the FPGA, and simultaneously performing sorting and deduplication processing on the comparison result: correcting a base quality value of the comparison result after sorting and deduplication processing; and detecting a mutation result according to the corrected comparison result. The described method may save program running time, save calculation costs, and improve resequencing efficiency.

Abstract: Provided is a transcriptome assembly method, comprising the following steps of: constructing a sequencing sample transcriptome read into a de Brujin graph; performing filtering and linearization processing on the de Brujin graph, so as to form continuous contigs; obtaining association among the contigs, and filtering association data; performing linearization processing on a continuous sequence without bifurcation; outputting a contig sequence; comparing the read and an end pairing read with the output contig sequence, so as to obtain information between the read and the contig; establishing connections among the contigs, so as to construct a graph with the contigs as points and the connections as edges; pre-processing and dividing the obtained graph, so as to obtain independent sub-graphs; and outputting a transcript according to the sub-graphs. Further provided is a transcriptome assembly system based on the method.