Abstract: A method and an apparatus for calibrating an image sensor array in a microscopic imaging system are provided. The method includes: performing a vignetting effect calibration and correction on the image sensor array, such that pixels acquired by all sub-field-of-view image sensors for capturing a scene with a same radiant exitance have a same gray level; performing a light encoding on a temporal-spatial union structure using a spatial light modulator, to establish correspondences of a plurality of feature points between an image space and a physical space; performing a light decoding on the temporal-spatial union structure, to acquire pixel coordinates of the plurality of feature points in an image plane and physical coordinates of the plurality of feature points in a plane of the spatial light modulator; acquiring a homography relationship according to the pixel coordinates and the physical coordinates, acquiring a global coordinate mapping according to the homography relationship.

Abstract: The present disclosure relates to Fourier ptychographic microscopy systems and methods. The system includes: a three-color LED array; a microscope, configured to obtain image information with multi-wavelength, and magnify to generate magnified image information; an RGB camera configured to acquire a first color image with a first resolution based on the magnified image information; and a controller, configured to synchronously control the three-color LED array and the RGB camera, in which the three-color LED array is further configured to display a plurality of illumination patterns, the RGB camera is further configured to acquire synchronously a plurality of first color images, and the controller is further configured to restore a single second image with a second resolution according to the plurality of first color images, and the first resolution is less than the second resolution. The present disclosure improves sampling speed.

Abstract: A method and an apparatus for calibrating an image sensor array in a microscopic imaging system are provided. The method includes: performing a vignetting effect calibration and correction on the image sensor array, such that pixels acquired by all sub-field-of-view image sensors for capturing a scene with a same radiant exitance have a same gray level; performing a light encoding on a temporal-spatial union structure using a spatial light modulator, to establish correspondences of a plurality of feature points between an image space and a physical space; performing a light decoding on the temporal-spatial union structure, to acquire pixel coordinates of the plurality of feature points in an image plane and physical coordinates of the plurality of feature points in a plane of the spatial light modulator; acquiring a homography relationship according to the pixel coordinates and the physical coordinates, acquiring a global coordinate mapping according to the homography relationship.

Abstract: The present disclosure relates to Fourier ptychographic microscopy systems and methods. The system includes: a three-color LED array; a microscope, configured to obtain image information with multi-wavelength, and magnify to generate magnified image information; an RGB camera configured to acquire a first color image with a first resolution based on the magnified image information; and a controller, configured to synchronously control the three-color LED array and the RGB camera, in which the three-color LED array is further configured to display a plurality of illumination patterns, the RGB camera is further configured to acquire synchronously a plurality of first color images, and the controller is further configured to restore a single second image with a second resolution according to the plurality of first color images, and the first resolution is less than the second resolution. The present disclosure improves sampling speed.