Abstract: The present invention relates to the field of inland vessel identification and ranging technology, and discloses a method, system, medium, equipment and terminal for identifying and ranging inland vessels. In the stage of vessel identification, based on the classical YOLO-V4 network model, the MobileNetV1 network is used to replace the feature extraction network CSPDarknet53 of the YOLO-V4 model; In the stage of vessel ranging, a binocular stereo vision ranging model is established, and the FSRCNN is used for super-resolution reconstruction of the original image pairs to enhance the vessel feature information; the ORB algorithm is used to achieve feature detection and matching at the sub-pixel level to obtain the parallax value between image pairs, and the depth information of the vessel target is obtained by triangulation principle and coordinate conversion.

Abstract: Described herein is a novel method for generation of recombinant poxviruses using an E3 and K3 double deletion mutant virus as the parental virus for generation of recombinant viruses. Following allowing for crossing over between the parental virus and an insertion cassette including an orthopox K3 peptide and the gene of interest, recombinant viruses are selected by infecting a host cell line permissive for the orthopox K3 peptide but not for the E3 and K3 double mutant parental virus. It is also demonstrated that a specific small molecule inhibitor of NEDD8 activating enzyme, MLN4924, can completely block poxvirus K3 family protein mediated PKR degradation and virus replication.

Abstract: The present invention relates to the field of inland vessel identification and ranging technology, and discloses a method, system, medium, equipment and terminal for identifying and ranging inland vessels. In the stage of vessel identification, based on the classical YOLO-V4 network model, the MobileNetV1 network is used to replace the feature extraction network CSPDarknet53 of the YOLO-V4 model; In the stage of vessel ranging, a binocular stereo vision ranging model is established, and the FSRCNN is used for super-resolution reconstruction of the original image pairs to enhance the vessel feature information; the ORB algorithm is used to achieve feature detection and matching at the sub-pixel level to obtain the parallax value between image pairs, and the depth information of the vessel target is obtained by triangulation principle and coordinate conversion.

Abstract: Described herein is a novel method for generation of recombinant poxviruses using an E3 and K3 double deletion mutant virus as the parental virus for generation of recombinant viruses. Following allowing for crossing over between the parental virus and an insertion cassette including an orthopox K3 peptide and the gene of interest, recombinant viruses are selected by infecting a host cell line permissive for the orthopox K3 peptide but not for the E3 and K3 double mutant parental virus. It is also demonstrated that a specific small molecule inhibitor of NEDD8 activating enzyme, MLN4924, can completely block poxvirus K3 family protein mediated PKR degradation and virus replication.

Abstract: The severe acute respiratory syndrome virus (SARS) is a coronavirus that instigated regional epidemics in Canada and several Asian countries in 2003. The newly identified SARS coronavirus (SARS-CoV) can be transmitted among humans and cause severe or even fatal illnesses. As preventive vaccine development takes years to complete and adverse reactions have been reported to some veterinary coronaviral vaccines, anti-viral compounds must be relentlessly pursued. In this study, we analyzed the effect of aurintricarboxylic acid (ATA) on SARS-CoV replication in cell culture, and found that ATA could drastically inhibit SARS-CoV replication, with viral production being more than 1000 fold than that in the untreated control. ATA is also shown to be an effective anti-viral for several other viruses, including West Nile Virus and variola virus.