Abstract: Fluorescence-based techniques are the cornerstone of modern biomedical optics with applications ranging from bioimaging at various scales (organelle to organism) to detection and quantification of a wide variety of biological species of interest. However, feeble fluorescence signal remains a persistent challenge in meeting the ever-increasing demand to image, detect and quantify biological species of low abundance. Disclosed herein are simple and universal methods based on a flexible and conformal elastomeric film adsorbed with plasmonic nanostructures, referred to as “plasmonic skin” or “plasmonic patch”, that provide large and uniform enhancement of fluorescence on a variety of surfaces, through an “add-on-top” process.

Abstract: The present disclosure is directed to microneedle patches for direct sampling and ultrasensitive detection of protein biomarkers in dermal interstitial fluids. The microneedle patches are comprised of polymers with high protein absorption capability (e.g. polystyrene) and are modified with capture biorecognition elements that are specific to target analytes in the interstitial fluid (ISF). Systems and methods are further provided for detection of a target ISF analyte obtained by in vivo sampling of the ISF using a microneedle patch.

Abstract: Fluorescence-based techniques are the cornerstone of modern biomedical optics with applications ranging from bioimaging at various scales (organelle to organism) to detection and quantification of a wide variety of biological species of interest. However, feeble fluorescence signal remains a persistent challenge in meeting the ever-increasing demand to image, detect and quantify biological species of low abundance. Disclosed herein are simple and universal methods based on a flexible and conformal elastomeric film adsorbed with plasmonic nanostructures, referred to as “plasmonic skin” or “plasmonic patch”, that provide large and uniform enhancement of fluorescence on a variety of surfaces, through an “add-on-top” process.

Abstract: Described herein are methods and kits for performing plasmon-enhanced fluoro-dot assays. These assays enable observing a correlation between a chemical stimulus and a biological response of cultured cells in vitro.

Abstract: Fluorescence-based techniques are the cornerstone of modern biomedical optics with applications ranging from bioimaging at various scales (organelle to organism) to detection and quantification of a wide variety of biological species of interest. However, feeble fluorescence signal remains a persistent challenge in meeting the ever-increasing demand to image, detect and quantify biological species of low abundance. Disclosed herein are simple and universal methods based on a flexible and conformal elastomeric film adsorbed with plasmonic nanostructures, referred to as “plasmonic skin” or “plasmonic patch”, that provide large and uniform enhancement of fluorescence on a variety of surfaces, through an “add-on-top” process.

Abstract: Fluorescence-based techniques are the cornerstone of modern biomedical optics with applications ranging from bioimaging at various scales (organelle to organism) to detection and quantification of a wide variety of biological species of interest. However, feeble fluorescence signal remains a persistent challenge in meeting the ever-increasing demand to image, detect and quantify biological species of low abundance. Disclosed herein are simple and universal methods based on a flexible and conformal elastomeric film adsorbed with plasmonic nanostructures, referred to as “plasmonic skin” or “plasmonic patch”, that provide large and uniform enhancement of fluorescence on a variety of surfaces, through an “add-on-top” process.

Abstract: Disclosed herein is a fluorescent nanoconstruct that includes a plasmonic nanostructure having at least one localized surface plasmon resonance wavelength (?LSPR), at least one spacer coating, at least one fluorescent agent having a maximum excitation wavelength (?EX), and wherein the fluorescent nanoconstruct has a fluorescent intensity that is at least 500 times greater than a fluorescent intensity of the at least one fluorescent agent alone.

Abstract: The present disclosure is directed to refreshable biosensors and methods for synthesizing and refreshing same. In some embodiments, the refreshable biosensor comprises a plasmonic nanoparticle and a biorecognition element, wherein the biorecognition element is encapsulated with at least one of an organosilica polymer layer or a metal organic framework (MOF).

Abstract: The present disclosure is directed to microneedle patches for direct sampling and ultrasensitive detection of protein biomarkers in dermal interstitial fluids. The microneedle patches are comprised of polymers with high protein absorption capability (e.g. polystyrene) and are modified with capture biorecognition elements that are specific to target analytes in the interstitial fluid (ISF). Systems and methods are further provided for detection of a target ISF analyte obtained by in vivo sampling of the ISF using a microneedle patch.

Abstract: Fluorescence-based techniques are the cornerstone of modern biomedical optics with applications ranging from bioimaging at various scales (organelle to organism) to detection and quantification of a wide variety of biological species of interest. However, feeble fluorescence signal remains a persistent challenge in meeting the ever-increasing demand to image, detect and quantify biological species of low abundance. Disclosed herein are simple and universal methods based on a flexible and conformal elastomeric film adsorbed with plasmonic nanostructures, referred to as “plasmonic skin” or “plasmonic patch”, that provide large and uniform enhancement of fluorescence on a variety of surfaces, through an “add-on-top” process.

Abstract: Disclosed are diagnostic reagents having metal-organic frameworks and protection and enhancement of diagnostic reagents and materials using metal-organic frameworks.