Abstract: Methods of attaching a ligand to a surface are described that include contacting a surface with a substrate containing an amphiphilic comb polymer. The substrate is configured to provide a pattern of the amphiphilic comb polymer on a selected region of the surface. The substrate can be separated from the surface leaving the amphiphilic comb polymer on the selected region of the surface, thus providing a selected region of the surface having amphiphilic comb polymer on it. A ligand can then be deposited on the surface such that the selected region of the surface having the amphiphilic comb polymer is substantially free of the ligand.

Abstract: Methods of attaching a ligand to a surface are described that include contacting a surface having an amphiphilic comb polymer present thereon and having a first reactive moiety attached thereto with a substrate having at least one ligand thereon. The ligand can include a second reactive moiety, wherein the second reactive moiety of the biological ligand and the first reactive moiety of the amphiphilic comb polymer form a covalent bond. The substrate can be separated from the surface, thereby leaving the biological ligand covalently bound to the amphiphilic comb polymer.