Abstract: The present disclosure relates to an HCR lateral flow device for amplified detection of a target in a sample and methods of using the same. In some embodiments, the device and methods of use combine the sensitivity of HCR signal amplification with the simplicity of the lateral flow assay format to facilitate user-friendly amplified detection of a target in a sample.

Abstract: Described herein are conditional guide RNAs that change their activity status depending on the presence or absence of an input target, forming a complex with an RNA-guided effector and conditionally performing a downstream function on a target nucleic acid. Methods for conditionally performing a downstream function on the target nucleic acid using conditional guide RNAs are also described.

Abstract: The invention discloses a pharmaceutical formula used in photodynamic therapy (PDT) for cancer or precancerous lesions. The formula includes four components of disulfonic acid diphthaloyl iminomethyl dualistic substituted phthalocyanine zinc alkali salt. Furthermore, this invention also provides an industrial chromatography separation process. These components is obtained by separating ten cis isomers of ZnPcS2P2 from synthetic products and then separating among them 4 isomers with significant amphiphilic property. It is evident that 4 isomers of the invention shows improved drug affect compared to formulas containing those 10 cis-isomers.